Pre Mrna Processing
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Pre Mrna Processing
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Author : Angus I. Lamond
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Pre Mrna Processing written by Angus I. Lamond and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
he past fifteen years have seen tremendous growth in our understanding of T the many post-transcriptional processing steps involved in producing func tional eukaryotic mRNA from primary gene transcripts (pre-mRNA). New processing reactions, such as splicing and RNA editing, have been discovered and detailed biochemical and genetic studies continue to yield important new insights into the reaction mechanisms and molecular interactions involved. It is now apparent that regulation of RNA processing plays a significant role in the control of gene expression and development. An increased understanding of RNA processing mechanisms has also proved to be of considerable clinical importance in the pathology of inherited disease and viral infection. This volume seeks to review the rapid progress being made in the study of how mRNA precursors are processed into mRNA and to convey the broad scope of the RNA field and its relevance to other areas of cell biology and medicine. Since one of the major themes of RNA processing is the recognition of specific RNA sequences and structures by protein factors, we begin with reviews of RNA-protein interactions. In chapter 1 David Lilley presents an overview of RNA structure and illustrates how the structural features of RNA molecules are exploited for specific recognition by protein, while in chapter 2 Maurice Swanson discusses the structure and function of the large family of hnRNP proteins that bind to pre-mRNA. The next four chapters focus on pre-mRNA splicing.
Nuclear Pre Mrna Processing In Plants
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Author : A. S. N. Reddy
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-04-16
Nuclear Pre Mrna Processing In Plants written by A. S. N. Reddy and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-04-16 with Science categories.
During the last few years, tremendous progress has been made in understanding various aspects of pre-mRNA processing. This book, with contributions from leading scientists in this area, summarizes recent advances in nuclear pre-mRNA processing in plants. It provides researchers in the field, as well as those in related areas, with an up-to-date and comprehensive, yet concise, overview of the current status and future potential of this research in understanding plant biology.
Pre Mrna Processing
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Author : Angus I. Lamond
language : en
Publisher: Springer
Release Date : 2014-03-12
Pre Mrna Processing written by Angus I. Lamond and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-03-12 with Science categories.
he past fifteen years have seen tremendous growth in our understanding of T the many post-transcriptional processing steps involved in producing func tional eukaryotic mRNA from primary gene transcripts (pre-mRNA). New processing reactions, such as splicing and RNA editing, have been discovered and detailed biochemical and genetic studies continue to yield important new insights into the reaction mechanisms and molecular interactions involved. It is now apparent that regulation of RNA processing plays a significant role in the control of gene expression and development. An increased understanding of RNA processing mechanisms has also proved to be of considerable clinical importance in the pathology of inherited disease and viral infection. This volume seeks to review the rapid progress being made in the study of how mRNA precursors are processed into mRNA and to convey the broad scope of the RNA field and its relevance to other areas of cell biology and medicine. Since one of the major themes of RNA processing is the recognition of specific RNA sequences and structures by protein factors, we begin with reviews of RNA-protein interactions. In chapter 1 David Lilley presents an overview of RNA structure and illustrates how the structural features of RNA molecules are exploited for specific recognition by protein, while in chapter 2 Maurice Swanson discusses the structure and function of the large family of hnRNP proteins that bind to pre-mRNA. The next four chapters focus on pre-mRNA splicing.
Eukaryotic Mrna Processing
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Author : Adrian Krainer
language : en
Publisher: Oxford University Press
Release Date : 1997
Eukaryotic Mrna Processing written by Adrian Krainer and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Science categories.
This volume focuses on the major aspects of post-transcriptional mRNA processing in the nucleus of eukaryotic cells. Each of the described mRNA reactions is required for proper gene expression and can also serve as a control point for regulating the expression of many genes, for example duringembryonic development or in different cell types. The different chapters review the assembly of newly synthesized nuclear mRNA transcripts into hnRNP particles and catalytically active spliceosomes; the structure and mechanism of action of small nuclear ribonucleoprotein particles and proteinfactors that catalyse pre-mRNA splicing in mammalian cells and in yeast; the regulation of gene expression and generation of protein isoform diversity by alternative splicing; the mechanisms of 3' end cleavage and polyadenylation; the architecture of the cell nucleus in relation to these processesand to the localization of the relevant substrates and factors; the diverse mechanisms of RNA processing by ribozymes and their potential relevance for nuclear mRNA processing; the mechanism of spliced-leader addition by trans-splicing in nematodes and trypanosomes; and the process ofinsertion/deletion mRNA editing in kinetoplasmid protozoa. In each chapter, leading researchers have provided detailed, critical reviews of the history, experimental approaches, major advances, current ideas and models, as well as future directions, for each of these active areas of research.
Alternative Pre Mrna Splicing
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Author : Stefan Stamm
language : en
Publisher: John Wiley & Sons
Release Date : 2012-02-13
Alternative Pre Mrna Splicing written by Stefan Stamm and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-02-13 with Science categories.
Der definitive Leitfaden zum RNA-Splicing - ideal für alle Kliniker, die sich mit genetischen Erkrankungen befassen, insbesondere natürlich für RNA-Forscher. Website: www.wiley-vch.de/home/splicing
Spliceosomal Pre Mrna Splicing
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Author : Klemens J. Hertel
language : en
Publisher: Humana
Release Date : 2014-02-21
Spliceosomal Pre Mrna Splicing written by Klemens J. Hertel and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-21 with Medical categories.
Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing in vitro and in vivo manipulation of splicing events and high-throughput and bioinformatic analyses of alternative splicing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many new discoveries to come.
Mrna Processing And Metabolism
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Author : Daniel R. Schoenberg
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Mrna Processing And Metabolism written by Daniel R. Schoenberg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
Cells possess a wealth of posttranscriptional control mechanisms that impact on every conceivable aspect of the life of an mRNA. These processes are intimately intertwined in an almost baroque manner, where promoter context influences the recruitment of splicing factors, where the majority of pre-mRNAs undergo alternative splicing, and where proteins deposited during nuclear processing impact distal cytoplasmic processing, translation, and decay. If there is a unifying theme to mRNA Processing and Metabolism: Methods and Protocols, it is that mRNA processing and metabolism are integrated processes. Many of the techniques used to study mRNA have been described in a previous volume of this series (RNA–Protein Interaction Protocols, Susan Haynes, ed.) and specialized methods journals. In selecting topics for mRNA Processing and Metabolism: Methods and Protocols, I sought input on new and novel techniques and approaches that build on this foundation using technological advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference. I have tried not to bias this book toward any single model organism, and approaches described in the various chapters use yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells.
Investigating The Cotranscriptional Regulation Of Pre Mrna Splicing And 3 End Processing Microform
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Author : Emanuel Rosonina
language : en
Publisher: National Library of Canada = Bibliothèque nationale du Canada
Release Date : 2004
Investigating The Cotranscriptional Regulation Of Pre Mrna Splicing And 3 End Processing Microform written by Emanuel Rosonina and has been published by National Library of Canada = Bibliothèque nationale du Canada this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with categories.
Transcriptional activators play an important role in the assembly of the transcriptional apparatus at the promoter regions of genes. We examined whether activators participate in the coupling of transcription with pre-mRNA processing, as well. Strong activation domains resulted in higher levels of splicing and cleavage compared to weak activation domains when targeted to the promoter of reporter genes. Truncation of the CTD abrogated this effect indicating that the CTD is involved in mediating the effect of strong activators on efficient processing. Further exploration of this mechanism revealed that splicing factor PSF binds preferentially to strong activation domains, and stimulates splicing and cleavage in vivo, in a CTD dependent manner. Therefore, PSF likely mediates the effect of a strong activator on efficient processing, whereby strong activators facilitate the association of PSF with the elongation apparatus. Our findings therefore implicate both transcriptional activators and PSF in cotranscriptional splicing and 3 '-end formation. The production of a messenger RNA (mRNA) is a complex process that involves many concerted steps, including the processing of the primary transcript, or precursor mRNA (pre-mRNA). Processing involves capping, 3' -end cleavage and polyadenylation, and splicing of introns from within the pre-mRNA. Pre-mRNAs are transcribed by RNA polymerase II (pol II), and it has been found that pre-mRNA processing is coupled to transcription by pol II, facilitating efficient and coordinated production of mature mRNA. Here I report the results of investigations of cotranscriptional splicing and 3'-end formation of pre-mRNAs. The carboxyl-terminal domain (CTD) of pol II is a highly-repetitive sequence unique to pol II that plays key roles in coupling gene expression events leading to the production of mRNA. We examined the CTD requirement for processing of different pre-mRNAs by exploring the effect of CTD mutations on splicing and cleavage of reporter genes in mammalian cells. We found that the length, rather than the type of CTD repeats, can be the major determinant in the efficient processing of pre-mRNA substrates. Furthermore, our results suggest that the requirement for the CTD in pre-mRNA processing is dependent on sequences within the gene itself. The degree of CTD-dependence therefore appears to be pre-mRNA specific.
Transcription And Splicing
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Author : B. D. Hames
language : en
Publisher: Oxford University Press, USA
Release Date : 1988
Transcription And Splicing written by B. D. Hames and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Music categories.
This book gives a co-ordinated review of our present knowledge of eukaryotic RNA synthesis.
Spliceosomal Pre Mrna Splicing
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Author : Klemens J. Hertel
language : en
Publisher:
Release Date : 2014
Spliceosomal Pre Mrna Splicing written by Klemens J. Hertel and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Genetic engineering categories.
Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing in vitro and in vivo, manipulation of splicing events, and high-throughput and bioinformatic analyses of alternative splicing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many new discoveries to come.