Protein Purification
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Protein Purification
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Author : Robert K. Scopes
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-04-17
Protein Purification written by Robert K. Scopes and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-17 with Science categories.
New textbooks at alllevels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a reallack ofup-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. lt is not often easy to persuade such individuals to settime aside to help spread the knowledge they have accumu lated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases the availability of texts in active research areas should help stimulate the creation of new courses. NewYork CHARLES R. CANTOR Preface to the Second Edition The original plan for the first edition of this book was to title it Enzyme Purification: Princip/es and Practice.
Guide To Protein Purification
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Author : Richard R Burgess
language : en
Publisher: Academic Press
Release Date : 2009-11-03
Guide To Protein Purification written by Richard R Burgess and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-11-03 with Science categories.
Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. - Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology - Assembles chapters on both common and less common relevant techniques - Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
Protein Purification Protocols
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Author : Paul Cutler
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Protein Purification Protocols written by Paul Cutler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.
Guide To Protein Purification
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Author : Murray P. Deutscher
language : en
Publisher: Gulf Professional Publishing
Release Date : 1990
Guide To Protein Purification written by Murray P. Deutscher and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Proteins categories.
Guide to Protein Purification , designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features* Detailed procedures newly written for this volume* Extensive practical information* Rationale and strategies for protein and enzyme purification* Personal perspectives on enzyme purification by eminent researchersAmong the Topics Covered* General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structure.
Protein Analysis And Purification
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Author : I.M. Rosenberg
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-14
Protein Analysis And Purification written by I.M. Rosenberg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-14 with Mathematics categories.
This book is designed to be a practical progression of experimental techniques an investigator may follow when embarking on a biochemical project. The protocols may be performed in the order laid out or may be used inde pendently. The aim of the book is to assist a wide range of researchers. from the novice to the frustrated veteran, in the choice and design of experiments that are to be performed to provide answers to specific questions. The manual describes standard techniques that have been shown to work, as well as some newer ones that are beginning to prove important. By following the promi nently numbered steps. you can work your way through any protocol. whether it's a new technique or a task you've done before for which you need a quick review or updated methodology. This manual will assist the experimentalist in designing properly controlled experiments. There will be no advice for dealing with specific pieces of equip ment other than encouragement to read the manual, if you can find it. Through out all manipulations try to be objective. Be on the lookout for unexpected findings. You will learn the most from unexpected results. and they are often the beginning of the next project. It is never possible to record too much in your lab notebook. Do not get discouraged. Remember, things will not always run smoothly.
Protein Purification
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Author : Philip L.R. Bonner
language : en
Publisher: Garland Science
Release Date : 2007-06-30
Protein Purification written by Philip L.R. Bonner and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-06-30 with Science categories.
Protein Purification provides a guide to the major techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and its applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification.
Protein Purification
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Author : Jan-Christer Janson
language : en
Publisher: John Wiley & Sons
Release Date : 1998
Protein Purification written by Jan-Christer Janson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Medical categories.
This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
Protein Purification
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Author : K. S. Dayananda
language : en
Publisher: MV Learning
Release Date : 2015-08-30
Protein Purification written by K. S. Dayananda and has been published by MV Learning this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-08-30 with Science categories.
Provides a reference for the techniques and strategies involved in the purification of antibodies and recombinant proteins. The detailed practical guidelines given in the book are focused on laboratory scale purification. The book is designed to give the laboratory worker the information needed to undertake the challenge of designing a strategy to produce a purified protein from a crude mixture or cell debris.
Protein Purification
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Author : Philip Bonner
language : en
Publisher: Garland Science
Release Date : 2007-06-11
Protein Purification written by Philip Bonner and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-06-11 with Science categories.
Protein Purification provides a guide to the major techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and its applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification.
Membrane Protein Purification And Crystallization
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Author : Carola Hunte
language : en
Publisher: Elsevier
Release Date : 2003-01-05
Membrane Protein Purification And Crystallization written by Carola Hunte and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-01-05 with Science categories.
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. - Provides general guidelines and strategies for isolation and crystallization of membrane proteins - Gives detailed protocols that have wide application, and low specialized equipment needs - Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins - Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes - Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization - Presents a comprehensive guide to 2D-crystallization of membrane proteins