Protein Scaffolds For Cell Culture
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Protein Scaffolds For Cell Culture
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Author : Ana Isabel Machado Roque
language : en
Publisher:
Release Date : 2013
Protein Scaffolds For Cell Culture written by Ana Isabel Machado Roque and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.
Effects Of Three Dimensional Structure Of Tissue Scaffolds On Animal Cell Culture
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Author : Shubhayu Basu
language : en
Publisher:
Release Date : 2004
Effects Of Three Dimensional Structure Of Tissue Scaffolds On Animal Cell Culture written by Shubhayu Basu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Cell culture categories.
Abstract: Surface chemistry of the scaffold material, the fiber diameter, pore size, structure and configuration and porosity can regulate tissue development. The overall objective was to study the effect of the three dimensional structure of tissue scaffolds on animal cell culture. To achieve this, the growth of human astrocyte cells, secreting Glial-cell-line Derived Neurotrophic Factor (GDNF), in non-woven polyethylene terephthalate (PET) matrices was specifically targeted. The ability of GDNF to selectively nourish and regenerate dopaminergic neurons makes it an ideal candidate for the treatment of Parkinson's disease. On the basis of cell morphology, density and GDNF secretion, compressed PET matrices (porosity 88.8%, mean pore diameter 64 microns), treated with boiling sodium hydroxide, were found to be very effective in supporting high density growth of astrocytes. Most scaffolds used thus far, are anisotropic in their architecture and have a wide pore size distribution, making the task of identifying optimum values of the parameters important for tissue development even more difficult. However, microfabricated poly(lactic co-glycolic acid) (PLGA) scaffolds with a uniform structure supported a higher cell density, longer cell proliferation times than in non-uniform fibrous matrices, and induced less apoptosis, implying that uniformity in scaffold microstructure was a more important parameter than the mean pore size of the scaffolds. Cytocompatibility studies on PLGA scaffolds also revealed that cells could span in three-dimensional space over distances greater than four times their average length. To develop a fast convenient cell based model to study cell culture in three dimensional (3D) scaffolds, cells were transfected with an Enhanced Green Fluorescent Protein (EGFP) based reporter system. This allowed fast, sensitive and noninvasive monitoring of cell growth in suspension and could be used to detect the spatial distribution of these cells in 3D scaffolds. The fluorescence of these cells was linked to the S-phase of the cell cycle and decreased in a dosage dependent manner when the cells were treated with chemotherapeutic drugs 5-fluorouracil and doxorubicin, making these cells an ideal and reliable model for reporting cellular proliferation and drug effects on cell growth.
3d Cell Culture
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Author : Ranjna C. Dutta
language : en
Publisher: CRC Press
Release Date : 2018-05-11
3d Cell Culture written by Ranjna C. Dutta and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-11 with Medical categories.
3D cell culture is yet to be adopted and exploited to its full potential. It promises to upgrade and bring our understanding about human physiology to the highest level with the scope of applying the knowledge for better diagnosis as well as therapeutics. The focus of this book is on the direct impact of novel technologies and their evolution into viable products for the benefit of human race. It also describes the fundamentals of cell microenvironment to bring forth the relevance of 3D cell culture in tissue engineering and regenerative medicine. It discusses the extracellular matrix/microenvironment (ECM) and emphasizes its significance for growing cells in 3D to accomplish physiologically viable cell mass/tissue ex vivo. The book bridges the knowledge gaps between medical need and the technological applications through illustrations. It discusses the available models for 3D cell culture as well as the techniques to create substrates and scaffolds for achieving desired 3D microenvironment.
Microscale Additive Manufacturing Of Collagen Cell Culture Scaffolds
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Author : Alex E. Bell
language : en
Publisher:
Release Date : 2015
Microscale Additive Manufacturing Of Collagen Cell Culture Scaffolds written by Alex E. Bell and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
Communication between cells and their environment is a key influencing factor on cell fate in tissues. Through integrin receptors, cells can sense and respond to structural features of the extracellular matrix. Signaling-factor receptors can detect gradient changes in concentration of a multitude of soluble ligands. Both mechanical and biochemical signals are presented to cells in defined spatial patterns. One of the most important sets of signals for developing or regenerating tissue are those guiding the formation of a vascular supply. Vascular development is crucial to recapitulation of further tissue function. Replicating these 3D patterns in vitro is a considerable challenge for the long-term goal of engineering functional tissue replacements. Multiphoton crosslinking (MPC) is an additive manufacturing process using light-activated chemistry to produce crosslinking by the absorption of multiple photons. MPC is quadratically dependent on light intensity, allowing isolation of the reaction to the focal plane. With diffraction-limited optics, this provides 1 [micrometre] resolution. MPC has the potential to give unlimited 3D control of the structural and biochemical cues imparted by functional proteins and peptides at a sub-cellular scale. Potential benefits of MPC include both the ability to spatially isolate presentation of a given factor in a concentration-controlled manner and sequestering the factor to provide a sustained dose over an extended period of cell culture and tissue regeneration. The MPC technique is essentially applicable to any protein, as several amino acids can react with an excited radical species. MPC of protein substrates has not yet been utilized toward functional tissue engineering applications. This is due to multiple factors, including the need for the substrate protein to be soluble, form a stable 3D structure, and have the unexposed portion readily removed. Thus, MPC has been limited in use primarily to highly soluble proteins such as albumin, which is not effective as a tissue scaffold material. Patterning of functional proteins onto scaffolds using MPC has also been limited by the need for the unexposed materials to be removed from the scaffold following the patterning process. This has meant that the technique, thus far, is largely employed on synthetic hydrogels with peptide fragments. This dissertation encompasses studies that established new additive manufacturing methods for cellular scaffolds in dermal wound healing. Completion of this work has expanded the capability of MPC to allow 3D fabrication of type I collagen scaffolds with spatial control of local structural and biochemical signals at a sub-cellular scale. Initial studies were performed to assess the effectiveness of these scaffolds patterned with VEGF for the induction of vasculogenesis by spatially defining the presentation of vasculogenic signals. These materials were used in a form as near as possible to their native state in an attempt to present a signaling environment more similar to a native ECM. These studies were directed toward implementation in vasculogenic dermal wound scaffolds, but the methods used are generally applicable to creation of structural and chemical patterning in any TE/RM application.
Cells And Culture
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Author : Thomas Noll
language : en
Publisher: Springer Science & Business Media
Release Date : 2010-07-17
Cells And Culture written by Thomas Noll and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-07-17 with Medical categories.
Regeneration of tissue to replace damaged or injured tissue is the goal of t- sue engineering. Biomaterials like polyglycolic acid, collagen and small-intestinal submuscosa provide a temporary scaffold to guide new tissue growth and or- nization. Typically, they need to be biodegradable, showing good cell atta- ment and proliferation and they should possess appropriate mechanical properties (Kim et al. , 2000). Synthetic polymers ful ll most of these requirements but lack cell-adhesion peptides on their surface to enhance cell attachment. Ce- adhesion peptides are present in ECM proteins like collagen and elastin. Thus a synthetic polymer coated with ECM proteins would result in a scaffold that mimics the natural cellular environment with enhanced cell attachment and p- liferation. The new bioactive scaffold will be made by combining a synthetic polymer coated with a layer of recombinant ECM proteins produced by CHO cells. The rst step consists of identifying polymers that give best results in terms of CHO cell attachment and growth. Classical techniques to determine biomass are inappropriate to evaluate 3-D structures. Thus a screening system based on stable GFP expressing CHO cells was used to compare the different scaffolds. Simple uorescent measurement after cell lysis allows determining cell attachment and p- liferation on synthetic polymers. Finally CHO cells producing human recombinant collagen I and elastin were generated. We showed that both proteins are expressed and secreted by CHO DG44 cells. 2 Materials and Methods 2.
Development Of A Cell Culture Model To Investigate The Interaction Between Scaffold Protein Pdzk1 And Oxalate Transporter Slc26a6
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Author : Lea Marie Pot d'Or
language : en
Publisher:
Release Date : 2023
Development Of A Cell Culture Model To Investigate The Interaction Between Scaffold Protein Pdzk1 And Oxalate Transporter Slc26a6 written by Lea Marie Pot d'Or and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023 with categories.
Culture Of Cells For Tissue Engineering
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Author : Gordana Vunjak-Novakovic
language : en
Publisher: John Wiley & Sons
Release Date : 2006-03-31
Culture Of Cells For Tissue Engineering written by Gordana Vunjak-Novakovic and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-03-31 with Science categories.
Step-by-step, practical guidance for the acquisition, manipulation,and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporatingthe principles of biology, chemistry, engineering, and medicine tocreate biological substitutes of native tissues for scientificresearch or clinical use. Specific applications of this technologyinclude studies of tissue development and function, investigatingdrug response, and tissue repair and replacement. This area israpidly becoming one of the most promising treatment options forpatients suffering from tissue failure. Written by leading experts in the field, Culture of Cellsfor Tissue Engineering offers step-by-step, practicalguidance for the acquisition, manipulation, and use of cell sourcesfor tissue engineering. It offers a unique focus on tissueengineering methods for cell sourcing and utilization, combiningtheoretical overviews and detailed procedures. Features of the text include: Easy-to-use format with a two-part organization Logically organized—part one discusses cell sourcing,preparation, and characterization and the second part examinesspecific engineered tissues Each chapter covers: structural and functional properties oftissues, methodological principles, culture, cellselection/expansion, cell modifications, cell seeding, tissueculture, analytical assays, and a detailed description ofrepresentative studies End-of-chapter features include useful listings of sources forreagents, materials, and supplies, with the contact details of thesuppliers listed at the end of the book A section of elegant color plates to back up the figures in thechapters Culture of Cells for Tissue Engineering givesnovice and seasoned researchers in tissue engineering an invaluableresource. In addition, the text is suitable for professionals inrelated research, particularly in those areas where cell and tissueculture is a new or emerging tool.
Silk Protein And Machine Learning In Cellular Tissue Engineering
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Author : Cong Sun
language : en
Publisher:
Release Date : 2022
Silk Protein And Machine Learning In Cellular Tissue Engineering written by Cong Sun and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with categories.
With new developments in the life sciences, the demand for 3D cell culture technology is increasing dramatically. The advantages of encapsulating cells in hydrogels to create 3D cell culture systems are clear, and in the future, 3D cell culture will gradually replace 2D cell culture to better mimic the states of cell growth in vivo. Our focus in tissue engineering is on the combination of 3D cell culture with silk-based biomaterials for further applications in disease treatment. We are also evaluating the biomedical localization of silk-based biomaterials for applications in skeletal muscle and cardiovascular diseases, as well as machine learning for investigation in the quantitative evaluation of vascular growth and overall blood vessel numbers within histological sections. The goal is to develop an engineered tissue using silk-based biomaterials as a base scaffold and to combine the biomaterial scaffold with in vitro 3D cell culture to develop an in vitro 3D silk culture system that can be used as a clinical application. Machine learning is also used to accelerate the quantitative analysis of angiogenesis in the implanted scaffolds and ultimately determine whether these silk scaffolds are suitable for skeletal or cardiac muscle treatment with repair.
Cells And Culture
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Author : Thomas Noll
language : en
Publisher: Springer
Release Date : 2016-05-01
Cells And Culture written by Thomas Noll and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-01 with categories.
Regeneration of tissue to replace damaged or injured tissue is the goal of t- sue engineering. Biomaterials like polyglycolic acid, collagen and small-intestinal submuscosa provide a temporary scaffold to guide new tissue growth and or- nization. Typically, they need to be biodegradable, showing good cell atta- ment and proliferation and they should possess appropriate mechanical properties (Kim et al., 2000). Synthetic polymers ful ll most of these requirements but lack cell-adhesion peptides on their surface to enhance cell attachment. Ce- adhesion peptides are present in ECM proteins like collagen and elastin. Thus a synthetic polymer coated with ECM proteins would result in a scaffold that mimics the natural cellular environment with enhanced cell attachment and p- liferation. The new bioactive scaffold will be made by combining a synthetic polymer coated with a layer of recombinant ECM proteins produced by CHO cells. The rst step consists of identifying polymers that give best results in terms of CHO cell attachment and growth. Classical techniques to determine biomass are inappropriate to evaluate 3-D structures. Thus a screening system based on stable GFP expressing CHO cells was used to compare the different scaffolds. Simple uorescent measurement after cell lysis allows determining cell attachment and p- liferation on synthetic polymers. Finally CHO cells producing human recombinant collagen I and elastin were generated. We showed that both proteins are expressed and secreted by CHO DG44 cells. 2 Materials and Methods 2.
Scaffolds For Tissue Engineering
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Author : Claudio Migliaresi
language : en
Publisher: CRC Press
Release Date : 2014-06-10
Scaffolds For Tissue Engineering written by Claudio Migliaresi and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-10 with Medical categories.
Scaffolds for tissue engineering are devices that exploit specific and complex physical and biological functions, in vitro or in vivo, and communicate through biochemical and physical signals with cells and, when implanted, with the body environment. Scaffolds are produced mainly with synthetic materials, and their fabrication technologies are deri