Protocol Handbook For Cancer Biology

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Protocol Handbook For Cancer Biology

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Author : Gauri Misra
language : en
Publisher: Academic Press
Release Date : 2021-02-12

Protocol Handbook For Cancer Biology written by Gauri Misra and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-02-12 with Science categories.

Protocol Handbook for Cancer Biology brings together a comprehensive collection of the methods used for cancer assessment, diagnostics, and therapeutics. Various protocols are discussed along with alternative strategies, including the advantages and limitations of techniques that have been used in labs globally. These protocols are presented by cancer biology experts based on their real-world experience. The protocols in this book will be a valuable resource for cancer researchers and graduate students, who can utilize the techniques described to conduct research more efficiently and successfully. - Presents comprehensive protocols used for cancer assessment, diagnostics, and therapeutics all in one place - Encompasses alternative strategies considering the requirements of the end user and taking into consideration diverse research settings - Discusses limitations and advantages of each method in experimental design and execution, thus saving time during the research process

The Proteomics Protocols Handbook

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Author : John M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-09

The Proteomics Protocols Handbook written by John M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-09 with Science categories.

Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Lab Ref

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Author : Jane Roskams
language : en
Publisher: CSHL Press
Release Date : 2002

Lab Ref written by Jane Roskams and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Medical categories.

Table of contents: Section 1 Most Commonly Used Solutions A. Stock Solutions, 1 B. Biological Buffers, 13 C. Proteins, Enzymes, and Antibiotics, 27 D. Reagents for the Analysis, Labeling, and Synthesis of Nucleic Acids, 35 Section 2 Macromolecular Preparation and Purification Reagents A. DNA, 43 B. RNA, 47 C. Protein, 53 Section 3 Electrophoretic Separation of Proteins and Nucleic Acids A. Electrophoresis of DNA, RNA, and Protein, 63 B. Transfer, Hybridization, and Screening of DNA, RNA, and Protein, 81 Section 4 Visualizing Genes and Gene Products A. Use of Antibodies for Immunochemical Approaches: A Guide, 95 B. Fixatives, 101 C. Cytological Stains, Chromogen Substrates, and Fluorophores, 105 D. Mounting Media, 119 E. Microscopy Information, 123 Section 5 Specialized Media, Buffers, and Reagents A. Most Commonly Used Bacterial Media and Solutions, 133 B. Yeast, 139 C. Xenopus, 155 D. Mammalian Cell Culture, 161 Section 6 Storage and Shipment of Biological Samples, 169.

Cell Biology Protocols

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Author : J. Robin Harris
language : en
Publisher:
Release Date : 2006-03-06

Cell Biology Protocols written by J. Robin Harris and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-03-06 with Science categories.

Cell biology involves a range of techniques for examining how cells function, regulate their own behavior, and interact with their neighbors. This book, the first in a series of five comprehensive methods handbooks, covers key protocols in this dynamic field including cellular organelles, cell growth and division, cell movement, cell signaling, and cell death. Because molecular biology approaches are widely used in cell biology, a few essential techniques from that field are also included.

Cancer Stem Cells And Signaling Pathways

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Author : Surajit Pathak
language : en
Publisher: Elsevier
Release Date : 2023-11-01

Cancer Stem Cells And Signaling Pathways written by Surajit Pathak and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-11-01 with Science categories.

Cancer Stem Cells and Signaling Pathways explores the mechanism of cancer stem cell signaling pathways in cancer advancement, serving as an ideal reference guide for investigators with valuable answers to problems associated with anti-cancer treatments and an understanding of the crosstalk among the signaling pathways in CSC regulation. Despite recent advances in tumor therapy and a greater understanding of their biology, tumors continue to be a prevalent and fatal worldwide. According to a novel paradigm in tumor biology, a small percentage of the cells within a tumor known as cancer stem cells (CSCs) are predicted as a crucial population for tumor progression. - Presents cancer stem characteristics and their signaling pathways - Highlights the use of animal models for cancer research, the use of genetically modified stem cells to deliver antitumor agents, and the significance of stem cell signaling in drug discovery - Covers basic and translational research appropriate for scientists and clinicians exploring alternative treatments for various cancers

Cancer Stem Cells Basic Concept And Therapeutic Implications

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Author : Farhadul Islam
language : en
Publisher: Springer Nature
Release Date : 2023-07-26

Cancer Stem Cells Basic Concept And Therapeutic Implications written by Farhadul Islam and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-07-26 with Medical categories.

This book comprehensively reviews the role of cancer stem cells (CSCs) in cancer initiation, progression, and resistance to anticancer therapies. The initial chapters examine the methods and procedure of the detection, isolation, and characterization of CSCs. It also introduces various epigenetic pathways that contribute to cancer initiation and tumorigenesis, particularly regarding the maintenance and survival of CSCs. It also explores the role of CSCs metabolism and the mechanisms of metabolic plasticity of CSCs in cancer biology. Further, it also presents the implications of CSCs on the origin of tumor heterogeneity and on heterogeneity of the therapeutic response. Towards the end, this book highlights the different immunotherapeutic approaches targeting CSCs with the potential of strongly improving cancer outcomes. This book offers a broad framework to scientists and clinicians into the state-of-the-art knowledge on cancer stem cell biology and highlights their therapeutic implications. ​

Flow Cytometry Applications In Cellular And Molecular Toxicology

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Author : AB Pant
language : en
Publisher: Springer Nature
Release Date : 2025-01-22

Flow Cytometry Applications In Cellular And Molecular Toxicology written by AB Pant and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-01-22 with Medical categories.

The book explores the role of flow cytometry in varied fields, from clinical diagnosis to toxicology. This comprehensive book offers insights into biomarkers, cellular analysis, and safety evaluations. Organized into fifteen chapters, this book explores flowcytometry's historical journey, scientific validation, and implementation in toxicity studies with case studies, technical and applied approaches, pictorial representations, informative tables, and simple language, It will be an invaluable resource for researchers, academia, biopharma industries, graduate and postgraduate students, Ph.D., and post-doctoral fellows working in the fields of toxicology/biosafety, and biomedical research.

The Cancer Handbook

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Author : Malcolm Alison
language : en
Publisher: Nature Publishing Group
Release Date : 2002

The Cancer Handbook written by Malcolm Alison and has been published by Nature Publishing Group this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Medical categories.

Provides a comprehensive overview of all major areas of cancer research and oncology. Bridging the gap between the molecular biology of cancer and clinical diagnosis and treatment, this online reference work provides a resource for medical and life sciences students, as well as all scientists, clinicians and researchers working in the cancer field and related biomedical areas.

Drosophila Protocols

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Author : William Sullivan
language : en
Publisher: CSHL Press
Release Date : 2000

Drosophila Protocols written by William Sullivan and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Medical categories.

This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators. Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable. The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.

A Guide To Sample Size For Animal Based Studies

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Author : Penny S. Reynolds
language : en
Publisher: John Wiley & Sons
Release Date : 2023-09-12

A Guide To Sample Size For Animal Based Studies written by Penny S. Reynolds and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-09-12 with Medical categories.

A Guide to Sample Size for Animal-based Studies Understand a foundational area of experimental design with this innovative reference Animal-based research is an essential part of basic and preclinical research, but poses a unique set of experimental design challenges. The most important of these are the 3Rs − Replacement, Reduction and Refinement − the principles comprising the ethical framework for humane animal-based studies. However, many researchers have difficulty navigating the design trade-offs necessary to simultaneously minimize animal use, and produce scientific information that is both rigorous and reliable. A Guide to Sample Size for Animal-based Studies meets this need with a thorough, accessible reference work to the subject. This book provides a straightforward systematic approach to “rightsizing” animal-based experiments, with sample size estimates based on the fundamentals of statistical thinking: structured research questions, variation control and appropriate design of experiments. The result is a much-needed guide to planning animal-based experiments to ensure scientifically valid and reliable results. This book offers: Step-by-step guidance in diverse methods for approximating and refining sample size Detailed treatment of research topics specific to animal-based research, including pilot, feasibility and proof-of-concept studies Sample size approximation methods for different types of data − binary, continuous, ordinal, time to event − and different study types − description, comparison, nested designs, reference interval construction and dose-response studies Numerous worked examples, using real data from published papers, together with SAS and R code A Guide to Sample Size for Animal-based Studies is a must-have reference for preclinical and veterinary researchers, as well as ethical oversight committees and policymakers.