Protocols For Gene Transfer In Neuroscience
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Protocols For Gene Transfer In Neuroscience
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Author : P. R. Lowenstein
language : en
Publisher:
Release Date : 1996-07-25
Protocols For Gene Transfer In Neuroscience written by P. R. Lowenstein and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-07-25 with Medical categories.
Protocols for Gene Transfer in Neuroscience Towards Gene Therapy of Neurological Disorders Edited by P. R. Lowenstein University of Manchester, UK and L. W. Enquist Princeton University, USA The brain and the cells that compose it have always presented unique barriers to any kind of outside interventions, including drug delivery, surgical procedures and the genetic manipulation of cells. However, these barriers are now being bypassed by the new technology of gene delivery. Protocols for Gene Transfer in Neuroscience is the first practical guide to gene delivery, providing over 150 easy to follow, step-by-step protocols, together with troubleshooting hints and the theoretical background needed for neuroscientists to understand, adapt and expand the technology as needed. Written by a team of international expert neuroscientists, geneticists, molecular biologists and biochemists, Protocols for Gene Transfer in Neuroscience describes the latest techniques from the top gene-delivery labs, ranging from physical transfection techniques, through the use of a wide range of viral vectors, to retrovirally transduced cell and bone marrow transplantation. Several variations of a single protocol are given, thus demonstrating how different labs have solved a common problem in different ways. The protocols are also indexed and cross-referenced so that an investigator can find all the details required for a particular problem.
Gene Transfer And Expression Protocols
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Author : Edward J. Murray
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Gene Transfer And Expression Protocols written by Edward J. Murray and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
Biology is the study of living things. The classical approach might be described as holistic and descriptive, whereas the modern molecular - proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. The new science of molecular biology has introduced techniques that permit the design of such experimental models. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line . Subsequent analysis of the proteins expressed from the genes under study results in the identification of the regulatory DNA sequences .
Gene Therapy Protocols
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Author : Joseph LeDoux
language : en
Publisher: Humana Press
Release Date : 2014-11-02
Gene Therapy Protocols written by Joseph LeDoux and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-11-02 with Medical categories.
In this book internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors and Design and Characterization of Gene Transfer Vectors. Readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for applications in stem cell biology, cancer research and infectious disease.
Gene Therapy For Neurological Disorders
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Author : Fredric P. Manfredsson
language : en
Publisher:
Release Date : 2016
Gene Therapy For Neurological Disorders written by Fredric P. Manfredsson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with Biomedicine categories.
This volume provides a clear and detailed roadmap of how to design and execute a gene therapy experiment in order to obtain consistent results. Chapters in this book disseminate bits of unknown information that are important to consider during the course of experimentation and will answer questions such as: What delivery vehicle do you use?; How will you ensure that your vector retains stability?; What expression system best fits your needs?; What route will you choose to deliver your gene therapy agent?; How will you model the neurodegenerative disorder that you aim to investigate, and what are the proven methods to treat these disorders in preclinical models? Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Gene Therapy for Neurological Disorders: Methods and Protocols, is a compilation of protocols and instructive chapters intended to give researchers, clinicians, and students of all levels, a foundation upon which future gene therapy experiments can be designed.
Brain Development
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Author : Simon G. Sprecher
language : en
Publisher: Humana Press
Release Date : 2013-09-19
Brain Development written by Simon G. Sprecher and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-09-19 with Medical categories.
Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes. Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models. Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.
Oversight And Review Of Clinical Gene Transfer Protocols
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Author : Committee on the Independent Review and Assessment of the Activities of the NIH Recombinant DNA Advisory Committee
language : en
Publisher: National Academies Press
Release Date : 2014-12-31
Oversight And Review Of Clinical Gene Transfer Protocols written by Committee on the Independent Review and Assessment of the Activities of the NIH Recombinant DNA Advisory Committee and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-12-31 with Medical categories.
Gene transfer research is a rapidly advancing field that involves the introduction of a genetic sequence into a human subject for research or diagnostic purposes. Clinical gene transfer trials are subject to regulation by the U.S. Food and Drug Administration (FDA) at the federal level and to oversight by institutional review boards (IRBs) and institutional biosafety committees (IBCs) at the local level before human subjects can be enrolled. In addition, at present all researchers and institutions funded by the National Institutes of Health (NIH) are required by NIH guidelines to submit human gene transfer protocols for advisory review by the NIH Recombinant DNA Advisory Committee (RAC). Some protocols are then selected for individual review and public discussion. Oversight and Review of Clinical Gene Transfer Protocols provides an assessment of the state of existing gene transfer science and the current regulatory and policy context under which research is investigated. This report assesses whether the current oversight of individual gene transfer protocols by the RAC continues to be necessary and offers recommendations concerning the criteria the NIH should employ to determine whether individual protocols should receive public review. The focus of this report is on the standards the RAC and NIH should use in exercising its oversight function. Oversight and Review of Clinical Gene Transfer Protocols will assist not only the RAC, but also research institutions and the general public with respect to utilizing and improving existing oversight processes.
Gene Transfer And Therapy In The Nervous System
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Author : Fred H. Gage
language : en
Publisher: Springer
Release Date : 2011-12-28
Gene Transfer And Therapy In The Nervous System written by Fred H. Gage and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-12-28 with Medical categories.
Neural Development
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Author : Renping Zhou
language : en
Publisher: Humana Press
Release Date : 2013-05-17
Neural Development written by Renping Zhou and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-05-17 with Medical categories.
Understanding the molecular and cellular mechanisms underlying the development of specific neural circuits is not just an intellectual curiosity but also central to our ability to develop therapeutic approaches to repair damaged pathways in the future. In Neural Development: Methods and Protocols, experts in the field contribute commonly used protocols to facilitate future research in developmental neuroscience. Split into four convenient sections, this detailed volume covers techniques of culturing neurons and glia as well as their growth and differentiation, methods of gene delivery and down regulation, protocols for analyzing axon growth and guidance plus synapse formation, and, finally, basic methods to analyze brain morphology and axon pathways in developing animals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and accessible, Neural Development: Methods and Protocols provides key guidance for students and postdoctoral fellows new to developmental neurobiology.
Gene Therapy Protocols
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Author : Joseph LeDoux
language : en
Publisher: Humana Press
Release Date : 2008-04-24
Gene Therapy Protocols written by Joseph LeDoux and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-04-24 with Medical categories.
In this book internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors and Design and Characterization of Gene Transfer Vectors. Readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for applications in stem cell biology, cancer research and infectious disease.
Gene Transfer And Therapy In The Nervous System
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Author : Fred Gage
language : en
Publisher: Springer Verlag
Release Date : 1992-01-01
Gene Transfer And Therapy In The Nervous System written by Fred Gage and has been published by Springer Verlag this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992-01-01 with Medical categories.