Protocols For Nucleic Acid Analysis By Nonradioactive Probes
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Protocols For Nucleic Acid Analysis By Nonradioactive Probes
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Author : Elena Hilario
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Protocols For Nucleic Acid Analysis By Nonradioactive Probes written by Elena Hilario and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Protocols for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative protocols required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive protocol implemented in the laboratory with minimum adaptation required and fastest time to results. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Nonisotopic Probing Blotting And Sequencing
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Author : Larry J. Kricka
language : en
Publisher: Elsevier
Release Date : 1995-06-01
Nonisotopic Probing Blotting And Sequencing written by Larry J. Kricka and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-06-01 with Science categories.
Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing. Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques. It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols. - Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein molecules - Gives protocols for nonisotopic DNA sequencing - new in this edition - Gives extensive, practical information - Presents background information for each method - Provides expert accounts from the inventor or developer of each method - Contains seven entirely new chapters - Covers all major types of nonisotopic procedures for labeling and detection
Molecular Biomethods Handbook
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Author : John M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-11-04
Molecular Biomethods Handbook written by John M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-04 with Medical categories.
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR. In Part B, protein and cell-based methods are outlined, in subjects ranging from protein engineering to high throughput screening. Written by a well-established panel of research scientists, Molecular Biomethods Handbook, 2nd Edition provides an up to date collection of methods used regularly in the authors’ own research programs. This book will prove to be an invaluable reference for those engaged in or entering the field of molecular biology, and will provide the necessary background for those interested in setting up and using the latest molecular techniques.
Handbook Of Nucleic Acid Purification
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Author : Dongyou Liu
language : en
Publisher: CRC Press
Release Date : 2009-01-14
Handbook Of Nucleic Acid Purification written by Dongyou Liu and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-14 with Science categories.
An Indispensable Roadmap for Nucleic Acid Preparation Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic a
Genetics Manual
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Author : G. P. Rdei
language : en
Publisher: World Scientific
Release Date : 1998
Genetics Manual written by G. P. Rdei and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Science categories.
"Redei has created an outstanding compendium of genetics. Arranged as a dictionary, the book is almost an encyclopedic collection of terms & concepts ... The author has managed to define terms with appropriate mixtures of depth & detail for the researcher, along with clarity useful for the nonexpert." Choice, 1998
Capillary Electrophoresis Guidebook
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Author : Kevin D. Altria
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Capillary Electrophoresis Guidebook written by Kevin D. Altria and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
This book is intended to be a working guide to the operation of capillary electrophoresis (CE) instrumentation. Since CE is still a rap idly maturing technique, detailed validated protocols are not widely established. Therefore, extensive experimental procedures are not pro vided for individual analyses. The intention is to provide general guide lines on the principles and practice of CE and to give an overview of the specific technologies and important application areas. Part I provides operating instructions for standard commercially available instruments. Guidelines are included for activities such as changing capillaries, method development, quantitative procedures, optimization of precision and sensitivity, and the validation of meth ods, fraction collection, and troubleshooting, as well as a quick guide to running a separation. The application range of CE is possibly the most diverse of all analytical techniques and ranges from large, complex macromolecules, such as proteins and nucleic acids, to small solutes, such as organic drugs and inorganic anions and cations.
Basic Dna And Rna Protocols
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Author : Adrian J. Harwood
language : en
Publisher:
Release Date : 1996-02-19
Basic Dna And Rna Protocols written by Adrian J. Harwood and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-02-19 with Medical categories.
In Basic DNA and RNA Protocols experienced researchers and innovators present an essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more. Each protocol represents a time-tested, step-by-step recipe that creates an understanding of the procedure, easily reproducible results, and confidence that the procedure will work. Its outstanding, thoroughly up-to-date, and eminently practical protocols make it an essential tool for today's molecular biologist.
Computer Analysis Of Sequence Data Part Ii
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Author : Annette M. Griffin
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Computer Analysis Of Sequence Data Part Ii written by Annette M. Griffin and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
DNA sequencing has become increasingly efficient over the years, resulting in an enormous increase in the amount of data gener ated. In recent years, the focus of sequencing has shifted, from being the endpoint of a project, to being a starting point. This is especially true for such major initiatives as the human genome project, where vast tracts of DNA of unknown function are sequenced. This sheer volume of available data makes advanced computer methods essen tial to analysis, and a familiarity with computers and sequence analy sis software a vital requirement for the researcher involved with DNA sequencing. Even for nonsequencers, a familiarity with sequence analysis software can be important. For instance, gene sequences already present in the databases can be extremely useful in the design of cloning and genetic manipulation experiments. This two-part work on Computer Analysis of Sequence Data is designed to be a practical aid to the researcher who uses computers for the acquisition, storage, or analysis of nucleic acid (and/or pro tein) sequences. Each chapter is written such that a competent scien tist with basic computer literacy can carry out the procedure successfully at the first attempt by simply following the detailed prac tical instructions that have been described by the author. A Notes section, which is included at the end of each chapter, provides advice on overcoming the common problems and pitfalls sometimes encoun tered by users of the sequence analysis software.
Human Molecular Biology Laboratory Manual
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Author : Stefan Surzycki
language : en
Publisher: John Wiley & Sons
Release Date : 2008-04-15
Human Molecular Biology Laboratory Manual written by Stefan Surzycki and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-04-15 with Science categories.
Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use
Molecular Dissection Of Complex Traits
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Author : Andrew H. Paterson
language : en
Publisher: CRC Press
Release Date : 2019-09-17
Molecular Dissection Of Complex Traits written by Andrew H. Paterson and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-09-17 with Science categories.
In the past 10 years, contemporary geneticists using new molecular tools have been able to resolve complex traits into individual genetic components and describe each such component in detail. Molecular Dissection of Complex Traits summarizes the state of the art in molecular analysis of complex traits (QTL mapping), placing new developments in thi