Pseudomonas Methods And Protocols
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Pseudomonas Methods And Protocols
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Author : Alain Filloux
language : en
Publisher: Humana
Release Date : 2016-09-03
Pseudomonas Methods And Protocols written by Alain Filloux and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-09-03 with Science categories.
In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings.
Pseudomonas Aeruginosa
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Author : Giovanni Bertoni
language : en
Publisher: Springer Nature
Release Date : 2023-10-11
Pseudomonas Aeruginosa written by Giovanni Bertoni and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-10-11 with Science categories.
This book aims to provide methods, protocols, and discussion topics for those who wish to examine in depth the molecular mechanisms of adaptation and versality of bacteria and would like to envisage their evolution responses in the fast changing Antropocene.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Pseudomonas aeruginosa: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Glycoprotein Methods And Protocols
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Author : Anthony P. Corfield
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-26
Glycoprotein Methods And Protocols written by Anthony P. Corfield and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-26 with Science categories.
The mucins (mucus glycoproteins) have long been a complex corner of glycoprotein biology. While dramatic advances in the separation, structural an- ysis, biosynthesis, and degradation have marked the progress in general glycop- tein understanding, the mucins have lagged behind. The reasons for this lack of progress have always been clear and are only now being resolved. The mucins are very large molecules; they are difficult to separate from other molecules present in mucosal secretions or membranes; they are often degraded owing to natural protective functions or to isolation methodology and their peptide and oligos- charide structures are varied and complex. Understanding these molecules has demanded progress in several major areas. Isolation techniques that protect the intact mucins and allow dissociation from other adsorbed but discrete molecules needed to be developed and accepted by all researchers in the field. Improved methods for the study of very large molecules with regard to their aggregation and polymerization were also needed. Structural analysis of the peptide domains and the multitude of oligosaccharide chains was required for smaller sample sizes, for multiple samples, and in shorter time. In view of these problems it is perhaps not surprising that the mucins have remained a dilemma, of obvious biological importance and interest, but very difficult to analyze.
Cystic Fibrosis Methods And Protocols
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Author : William R. Skach
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Cystic Fibrosis Methods And Protocols written by William R. Skach and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
Since the cloning of the cystic fibrosis transmembrane conductance re- lator (CFTR) nearly a decade ago, cystic fibrosis (CF) research has witnessed a dramatic expansion into new scientific areas. Basic researchers, clinicians, and patients increasingly rely on fundamental techniques of genetics, molecular biology, electrophysiology, biochemistry, cell biology, microbiology, and immunology to understand the molecular basis of this complex disease. Research into the pathophysiology of CF has established numerous paradigms of ion channel dysfunction that extend from inflammation and infection in the airways of patients to basic mechanisms of protein processing and regulation in intracellular components. With these rapid advances has come an increasing need for research scientists to understand and utilize a growing array of basic laboratory tools. This volume of Methods in Molecular Medicine, Cystic Fibrosis Methods and Protocols satisfies that need by providing detailed protocols for the laboratory techniques used throughout CF research. From electrophysiology and cell biology, to animal models and gene therapy, the comprehensive set of methods covered here provide step-by-step instructions needed for investigators to incorporate new approaches into their research programs. Contributions have been chosen to reflect the rich diversity of techniques and to provide a cohesive framework for understanding challenges that are currently at the forefront of CF research. It is hoped that this volume will serve as a valuable reference that will not only foster interdisciplinary investigations into current problems encountered in CF, but also facilitate the translation of new scientific discoveries into clinical solutions.
Plant Metabolites Methods Applications And Prospects
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Author : Swapna Thacheril Sukumaran
language : en
Publisher: Springer Nature
Release Date : 2020-11-28
Plant Metabolites Methods Applications And Prospects written by Swapna Thacheril Sukumaran and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-11-28 with Science categories.
Food security and the medicinal needs of billions of people around the world are pressing global issues, and the biodiversity and sustainable utilization of plants is of great significance in this context. Further, ethnobotanical studies are vital in the discovery of new drugs from indigenous medicinal plants, and plants with industrially important metabolites need to be cultivated to meet the growing market demand. In addition, the production of plant metabolites under in vitro conditions also has tremendous possibilities. The totipotency of plant cells plays a valuable role in the sustainable utilization of plant resources through cell, tissue and organ culture. At the same time, production can be enhanced using productive cell lines, treatment with elicitors, changing nutritional parameters and metabolic engineering. This book provides state-of-the-art information on biodiversity, conservation, ethnobotany, various aspects of In vitro secondary metabolite production, bioprospecting from various plant groups and drug discovery. It also discusses methods of extracting and characterizing drug leads from plant sources.
A Complete Guidebook On Biofilm Study
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Author : Dijendra N. Roy
language : en
Publisher: Academic Press
Release Date : 2022-06-22
A Complete Guidebook On Biofilm Study written by Dijendra N. Roy and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-06-22 with Science categories.
A Complete Guidebook on Biofilm Study has emphasized the biofilm-related issues in the present context related to research and development. For this purpose, experimental design and relevant experimental protocols for the biofilm studies have been highlighted here. In addition to that, inhibitors from natural or synthetic sources against microbial biofilm development have been addressed. This approach has been further substantiated by bioinformatics as well as nanotechnology-based reports. Both, the image processing related to biofilm study and the characters of substratum associated with biofilm development have also been included for a better understanding of the beginners in this field. Further, how biofilm helps and/or hampers in food processing and waste management system, that discussion has been considered in this book. Similarly, human benefits from biofilm and reverse of it have also been included considering host-pathogen interaction, immunity aspects, and others. - Carrying huge resources/information/ideas in a compiled manner for biofilm study/work - Has highlighted how biofilm-related experiment has to be designed based on protocols - This book has focused majorly about biofilm-related gene regulation along with the development of different inhibitors for therapeutic aspects. This paradigm has been further discussed based on the nanotechnology and bioinformatics approach - Biofilm studies related to waste management, food processing, and image processing, which are newly upcoming have been emphasized in this book
9th International Conference On The Development Of Biomedical Engineering In Vietnam
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Author : Van Toi Vo
language : en
Publisher: Springer Nature
Release Date : 2023-10-18
9th International Conference On The Development Of Biomedical Engineering In Vietnam written by Van Toi Vo and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-10-18 with Technology & Engineering categories.
This book presents cutting-edge research and developments in the field of biomedical engineering, with a special emphasis on results achieved in Vietnam and neighboring low- and middle-income countries. Covering both fundamental and applied research, and focusing on the theme of “Translational Healthcare Technology from Advanced to Low and Middle Income Countries in the Era of Covid and Digital Transformation”, it reports on the design, fabrication, and application of low-cost and portable medical devices, biosensors, and microfluidic devices, on improved methods for biological data acquisition and analysis, on nanoparticles for biological applications, and on new achievements in biomechanics, tissue engineering, and regeneration. It describes the developments of molecular and cellular biology techniques, neuroengineering techniques, and statistical and computational methods, including artificial intelligence, for biomedical applications. It also discusses strategies to address some relevant issues in biomedical education and entrepreneurship. Gathering the proceedings of the 9th International Conference on The Development of Biomedical Engineering in Vietnam, BME 9, held on December 27-29, 2022, in Ho Chi Minh, Vietnam, the book offers important answers to current challenges in the field and a source of inspiration for scientists, engineers, and researchers with various backgrounds working in different research institutes, companies, and countries.
Ecometabolomics
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Author : Sumira Jan
language : en
Publisher: Academic Press
Release Date : 2019-03-10
Ecometabolomics written by Sumira Jan and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-10 with Science categories.
Ecometabolomics: Metabolic Fluxes versus Environmental Stoichiometry focuses on the interaction between plants—particularly plants that have vigorous secondary metabolites—and the environment. The book offers a comprehensive overview of the responses of the metabolome of organisms to biotic and abiotic environmental changes. It includes an introduction to metabolomics, summaries of metabolomic techniques and applications, studies of stress in plants, and insights into challenges. This is a must-have reference for plant biologists, plant biochemists, plant ecologists and phytochemists researching the interface between plants and the environment using metabolomics. - Provides an in-depth overview of the basics of the discipline, including non-targeted analysis and quantification of plant metabolites - Outlines the applications of various analytical techniques in comprehending the total metabolome of the organism - Covers both NMR and MS-based approaches
Microbial Based Biopesticides
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Author : Travis R. Glare
language : en
Publisher:
Release Date : 2016
Microbial Based Biopesticides written by Travis R. Glare and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with Life sciences categories.
"This volume focuses on the developmental areas of biopesticides: production, formulation, application and field efficacy. Chapters guide readers through methods and techniques on environmental, mammalian, safety, and registration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Microbial-Based Biopesticides aims to ensure successful results in the further study of this vital field."--
Electroporation Protocols For Microorganisms
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Author : Jac A. Nickoloff
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Electroporation Protocols For Microorganisms written by Jac A. Nickoloff and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Electroporation is one of the most widespread techniques used in modem molecular genetics. It is most commonly used to introduce DNA into cells for investigations of gene structure and function, and in this regard, electroporation is both highly versatile, being effective with nearly all species and cell types, and highly efficient. For many cell types, electroporation is either the most efficient or the only means known to effect gene transfer. However, exposure of cells to brief, hi- intensity electric fields has found broad application in other aspects of biological research, and is now routinely used to introduce other types of biological and analytic molecules into cells, to induce cell-cell fusion, and to transfer DNA directly between different species. The first seven chapters of Electroporation Protocols for Micro organisms describe the underlying theory of electroporation, the com mercially available instrumentation, and a number of specialized electroporation applications, such as cDNA library construction and interspecies DNA electrotransfer. Each of the remaining chapters pre sents a well developed method for electrotransformation of a particular bacterial, fungal, or protist species. These chapters also serve to intro duce those new to the field the important research questions that are currently being addressed with particular organisms, highlighting both the major advantages and limitations of each species as a model organ ism, and explaining the roles that electroporation has played in the development of the molecular genetic systems currently in use.