R Primer

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Nucleic Acid Amplification Technologies

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Author : Helen H. Lee
language : en
Publisher: Springer Science & Business Media
Release Date : 1997-07-15

Nucleic Acid Amplification Technologies written by Helen H. Lee and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-07-15 with Medical categories.

Providing current information and guidance on the uses of various nucleic acid amplification technologies for clinical laboratory diagnosis, this book goes beyond the Polymerase Chain Reaction to explore a broader range of important alternative DNA/RNA amplification methods including the Ligase Chain Reaction, Q[beta] Replicase Assays and TMA. There are many examples of specific applications of these technologies, discussions of yet unresolved issues and demonstrations of the relevance of these technologies to medical research and disease diagnostics. Individual chapters cover uses of these methods in clinical situations such as detection of food pathogens, viral infections, STDs, Mycobacteria drug resistance mutations, and heritable diseases. Automation, diagnostic test evaluation, and the synthesis of artificial DNA are also discussed. This book is designed for all biomedical scientists interested in the application of molecular biology to clinical diagnosis.

Transposable Elements

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Author : Miguel R. Branco
language : en
Publisher: Springer Nature
Release Date : 2022-11-30

Transposable Elements written by Miguel R. Branco and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-11-30 with Science categories.

The volume presents a small selection of state-of-the-art approaches for studying transposable elements(TE). Chapters guide readers through HTS-based approaches, bioinformatic tools, methods to studyTE protein complexes, and the functional impact on the host. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Transposable Elements: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Manual Of Molecular And Clinical Lab Immunology

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Author : Barbara Detrick
language : en
Publisher: American Society for Microbiology Press
Release Date : 2006-03-10

Manual Of Molecular And Clinical Lab Immunology written by Barbara Detrick and has been published by American Society for Microbiology Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-03-10 with Medical categories.

Introduces new material that reflects the significant advances and developments in the field of clinical laboratory immunology. • Provides a comprehensive and practical approach to the procedures underlying clinical immunology testing. • Emphasizes molecular techniques used in the field of laboratory immunology. • Updates existing chapters and adds significant new material detailing molecular techniques used in the field. • Presents guidelines for selecting the best procedures for specific situations and discusses alternative procedures. • Covers aspects of immunology related disciplines such as allergy, autoimmune diseases, cancers, and transplantation immunology.

Molecular Aspects Of Hematologic Malignancies

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Author : Michal Witt
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-06-30

Molecular Aspects Of Hematologic Malignancies written by Michal Witt and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-06-30 with Science categories.

This book provides a state-of-the-art approach to the molecular basis of hematologic diseases and its translation into improved diagnostics and novel therapeutic strategies. Several representative hemato-oncologic malignancies are analyzed in detail: acute lymphoblastic leukemia, acute myeloid leukemia, B-cell Non-Hodgkin lymphomas, multiple myeloma, chronic lymphocytic leukemia, chronic myeloid leukemia, myelodysplastic syndromes, and myeloproliferative neoplasms. Experts in the field describe the molecular methods applied for modern diagnostics and therapies, such as hematopoietic stem cell transplantation, donor recipient matching, banking of biological material, analyses of post-transplant chimerism, and minimal residual disease monitoring. The volume concludes with an extensive section comprising thorough step-by-step protocols of molecular techniques in hematology, all of them validated in the authors’ own laboratories.

Introduction To Molecular Biology

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Author : Oksana Ableitner
language : en
Publisher: Springer Nature
Release Date : 2022-01-07

Introduction To Molecular Biology written by Oksana Ableitner and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-07 with Science categories.

Oksana Ableitner offers a practical, clearly structured and easy to understand introduction to complicated definitions and structures in chemistry and molecular biology for work in the molecular biology laboratory. The author is guided by her experience in working with students and uses many illustrations to visualize abstract knowledge. An understanding of this matter is an essential basis for successful work with DNA and RNA in order to ensure high quality results. For responsible activities in application - such as genetic research or the determination of various pathogens - it is essential to be confident in dealing with the basics of these sensitive, fast and specific analytical methods. This Springer essential is a translation of the original German 2nd edition essentials, Einführung in die Molekularbiologie by Oksana Ableitner, published by Springer Fachmedien Wiesbaden GmbH, part of Springer Nature in 2018. The translation was done with the help of artificial intelligence (machine translation by the serviceDeepL.com). A subsequent human revision was done primarily in terms of content, so that the book will read stylistically differently from a conventional translation. Springer Nature works continuously to further the development of tools for the production of books and on the related technologies to support the authors.

Genetic Transformation Systems In Fungi Volume 1

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Author : Marco A. van den Berg
language : en
Publisher: Springer
Release Date : 2014-10-28

Genetic Transformation Systems In Fungi Volume 1 written by Marco A. van den Berg and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-10-28 with Science categories.

Several different transformation techniques have been developed over the years and readily shown to be decisive methods in fungal biotechnology. This book will cover the basics behind the most commonly used transformation methods, as well as associated tools and techniques. Each chapter will provide protocols along with examples used in laboratories worldwide. ​ Not only will this text provide a detailed background on applications in industrial and pharmaceutical relevant microbes, but also the importance of fungal pathogens in agricultural production (Phytophthora and Botrytis) and mammalian infection (Penicillium marneffei and Candida). Genetic Transformation Systems in Fungi, Volume 1 provides in-depth coverage of how the transformation of DNA is used to understand the genetic basis behind these fungal traits.

Molecular Diagnostics

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Author : E. van Pelt-Verkuil
language : en
Publisher: Springer
Release Date : 2019-06-03

Molecular Diagnostics written by E. van Pelt-Verkuil and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-03 with Medical categories.

The books Molecular Diagnostics Part 1 and 2 provide a comprehensive and practical overview of the state-of-the-art molecular biological diagnostic strategies that are being used in a wide variety of disciplines. The editors and experts in their respective fields have combined their knowledge to write these two books. Many years of experience in the development, application and quality control of molecular diagnostic methods is reflected herewith. Molecular Diagnostics Part 1 is dedicated to the theoretical backgrounds of the technologies often applied in molecular diagnostics, in which nucleic acid amplification methods (such as real-time PCR), sequencing and bioinformatics are the basic tools. The assay design and -development, combined with items of trouble-shooting are described in detail. As a foundation of reliable molecular diagnostic assays, the quality control required for validation, implementation and performance of molecular diagnostic assays is thoroughly discussed. This book also provides extensive information for those working with molecular techniques in a wide variety of research applications using conventional and real-time PCR technology, Sanger and high throughput sequencing techniques, and bioinformatics. Molecular Diagnostics Part 2 highlights the applications of the molecular diagnostic methods in the various diagnostic laboratories, comprising: - Clinical microbiology - Clinical chemistry - Clinical genetics - Clinical pathology - Molecular hematopathology - Veterinary health - Plant health - Food safety Both full-colour and well-illustrated books are particularly valuable for students, clinicians, scientists and other professionals who are interested in (designing) molecular diagnostic methods and for those who wish to broaden their knowledge on the current molecular biological revolution. The information in the books highlights the trend ofthe integration of multiple (clinical) disciplines into one universal molecular laboratory.

Research Advances On Forest Tree Functional Genomics And Breeding

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Author :
language : en
Publisher: Frontiers Media SA
Release Date : 2025-01-17

Research Advances On Forest Tree Functional Genomics And Breeding written by and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-01-17 with Science categories.

Forest trees play a key role in terrestrial biodiversity and contribute to carbon sequestration. Forest trees also exhibit a rich array of agronomic and economic traits, making them ideal as important species for studying functional genes for the unique characteristics of forest tree species. Fast-growing forest plantations are sustainable feedstocks of plant biomass that can serve as substitutes for fossil carbon resources for materials, chemicals, and energy. The first annotated whole genome sequence of the model woody plant, Populus trichocarpa, was published in 2006. Since then, many forest tree species have been sequenced and annotated, which has facilitated the development of functional genomics, GWAS, genetic tools, and new biotechnologies in tree research. Targeted functional genomics allows us to identify genes that affect traits of interest, such as agronomic and economic traits, wood properties, and biotic stress resistance. Molecular biology-based biotechnologies allow us to manipulate these genes and traits. The breakthroughs in biotechnology approaches, together with accelerated genomic breeding, will be crucial for the functional revealing of important genes for key traits and the development of a new generation of woody crops, helping us to keep up with the growing demand for wood fiber and other bioproducts from forest trees. This Research Topic aims to explore the molecular mechanisms of key biological processes in model and non-model forest tree species with a combination of approaches of genetics, omics, and chemistry. Also, new technologies and resources used for forest tree species research are of equal importance to basic science. We encourage fundamental research in the field of forest tree genomics, genetic studies in revealing the genes and pathways associated with key agronomic and economic traits in forest trees, molecular mechanisms of secondary growth regulation in tree species, and the potential utilization of biotechnologies in genetic improvement of woody crops. We welcome all types of submissions, including original research, reviews, methodologies, mini-reviews, perspectives, and opinion articles in this field, including (but not limited to) research covering: • Genetic and functional characterization of genes regulating perennial tree secondary growth. • Genetic improvement in woody tree crops using gene editing and other modern biotechnologies. • Discovery, characterization, and application of germplasm resources with agronomic and economic traits. • New technologies and resources for forest tree research.

Laboratory Protocols In Fungal Biology

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Author : Vijai Kumar Gupta
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-09

Laboratory Protocols In Fungal Biology written by Vijai Kumar Gupta and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-09 with Science categories.

Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals. Coming Soon in the Fungal Biology series: Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2 van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi Schmoll, Dattenbock / Gene Expression Systems in Fungi Dahms / Advanced Microscopy in Mycology

Synthetic Biology

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Author : Christopher A. Voigt
language : en
Publisher: Academic Press
Release Date : 2011

Synthetic Biology written by Christopher A. Voigt and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Bioengineering categories.

Synthetic biology encompasses a variety of different approaches, methodologies and disciplines and many different definitions exist. This volume covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation.