Rapid Cycle Real Time Pcr

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Rapid Cycle Real Time Pcr Methods And Applications

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Author : W. Dietmaier
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29

Rapid Cycle Real Time Pcr Methods And Applications written by W. Dietmaier and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Medical categories.

Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier!

Rapid Cycle Real Time Pcr Methods And Applications

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Author : Carl Wittwer
language : en
Publisher: Springer
Release Date : 2012-12-06

Rapid Cycle Real Time Pcr Methods And Applications written by Carl Wittwer and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

Rapid Cycle Real-Time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes(HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!

Rapid Cycle Real Time Pcr Methods And Applications

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Author : U. Reischl
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Rapid Cycle Real Time Pcr Methods And Applications written by U. Reischl and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.

Rapid Cycle Real Time Pcr Methods And Applications

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Author : W. Dietmaier
language : en
Publisher:
Release Date : 2014-01-15

Rapid Cycle Real Time Pcr Methods And Applications written by W. Dietmaier and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-15 with categories.

Rapid Cycle Real Time Pcr

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Author : Meuer
language : en
Publisher: Springer Science & Business Media
Release Date : 2001

Rapid Cycle Real Time Pcr written by Meuer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with categories.

Rapid Cycle Real Time Pcr Methods And Applications

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Author : Udo Reischl
language : en
Publisher: Springer
Release Date : 2012-12-12

Rapid Cycle Real Time Pcr Methods And Applications written by Udo Reischl and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-12 with Science categories.

Rapid Cycle Real-Time PCR is a powerful analytical tool with broad application for the basic and applied life sciences. Compared with conventional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical microbiology laboratory as well as specific diagnostic protocols for the detection of viral, bacterial and fungal pathogens and genetically modified organisms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.

Real Time Pcr

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Author : M Tevfik Dorak
language : en
Publisher: Garland Science
Release Date : 2007-02-08

Real Time Pcr written by M Tevfik Dorak and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-02-08 with Science categories.

With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.

The Pcr Revolution

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Author : Stephen A. Bustin
language : en
Publisher: Cambridge University Press
Release Date : 2010

The Pcr Revolution written by Stephen A. Bustin and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Science categories.

Examines the latest innovations and the overall impact of PCR on areas of molecular research.

Gene Quantification

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Author : Francois Ferre
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Gene Quantification written by Francois Ferre and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.

Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.

Real Time Pcr

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Author : Kirstin J. Edwards
language : en
Publisher: Taylor & Francis
Release Date : 2004

Real Time Pcr written by Kirstin J. Edwards and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Polymerase chain reaction categories.