Receptor Localization
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Receptor Localization
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Author : Marjorie Ariano
language : en
Publisher: John Wiley & Sons
Release Date : 1998-07-16
Receptor Localization written by Marjorie Ariano and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-07-16 with Medical categories.
The detection of neurotransmitter receptor locations and distribution densities within the central nervous system and peripheral tissues is receiving intense attention within the neuroscience research community. Neurotransmitter receptors, which receive the chemical signals sent from one neuron to another, are critical links in a highly complex information-processing chain. Pinpointing receptor sites and systems is crucial for understanding neurological function as well as dysfunction. It is also essential for understanding how receptors process information when impacted by such substances as heroin or nicotine, or when affected by neurodegenerative disease. Receptor Localization: Laboratory Methods and Procedures is the first user-friendly guide to the latest techniques and approaches being employed to examine the localization of neurotransmitter receptors in the central nervous system and peripheral tissues. It covers detection methods that are applicable to a wide variety of receptor systems, ranging from genes and ligands to in vito receptors in individuals; and to numerous receptor subtypes, such as nicotine, muscarine, tackykinins, dopamine, adenosine, and GABA. The standard laboratory "recipes" or "tricks" employed in these detection methods are fully discussed, as are the advantages and limitations of each procedure. With contributions from leading experts and extensively illustrated, this book: * Discusses receptor ligand binding methods using irreversible and reversible compounds * Presents antireceptor antisera technology using synthetic peptides and fusion proteins at both the cellular and subcellular resolution levels * Examines molecular assessments of receptors * Describes in situ hybridization, reverse transcriptase-PCR, and fluorescent in situ transcription * Covers new visualization paradigms * Includes physiological analysis of receptor function, cellular detection in the brain slice, and cultured neurons * Discusses the use of PET and SPECT to assess in vivo receptor distributions in animals and humans Receptor Localization: Laboratory Methods and Procedures is an invaluable guide for researchers in the related fields of neurology, biochemistry, and pharmacology. Its lucid descriptions of new detection methods, inclusion of experimental examples, and emphasis on how these experimental approaches are applicable to particular research areas will appeal to both the experienced researcher and novice investigator.
Receptor Localization
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Author : Frances M. Leslie
language : en
Publisher:
Release Date : 1988
Receptor Localization written by Frances M. Leslie and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Medical categories.
Receptors
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Author : P. Michael Conn
language : en
Publisher:
Release Date : 1993
Receptors written by P. Michael Conn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Science categories.
Receptors initiate the means by which cellular regulators exert their actions on targets. Because of the central role of cell-cell communication and signal transduction, receptors are of intrinsic interest to neuroscientists. Receptor studies utilize both traditional methods of analysis and modern molecular techniques. Key Features * Methods presented for easy adaptation to new systems * Comprehensive protocols included for molecular techniques (PCR, cloning, transfection, coupling); techniques for the determination of receptor subclasses; techniques for localization (in situ hybridization, immunocytochemistry); ligand design (radioactive techniques, biotinylated techniques); receptor-associated kinase * Methodology described for the following receptors: acetylcholine, angiotensin II, bombesin/GRP, dopamine, GABA, G protein-coupled receptors, neurotensin, NGF, NPY, serotonin, somatostatin, tachykinin
Receptor Localization In The Cardiovascular System
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Author : Jennifer Anne Stephenson
language : en
Publisher:
Release Date : 1988
Receptor Localization In The Cardiovascular System written by Jennifer Anne Stephenson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Muscarinic receptors categories.
Odorant Receptor Localization To Olfactory Cilia Is Mediated By Odr 4 And Unc 101
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Author : Noelle Diane Dwyer
language : en
Publisher:
Release Date : 1998
Odorant Receptor Localization To Olfactory Cilia Is Mediated By Odr 4 And Unc 101 written by Noelle Diane Dwyer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Cell receptors categories.
Mechanism Of Acetylcholine Receptor Localization And Metabolic Stability At The Developing Nmj
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Author : John Burnley Rudell
language : en
Publisher:
Release Date : 2013
Mechanism Of Acetylcholine Receptor Localization And Metabolic Stability At The Developing Nmj written by John Burnley Rudell and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.
A key determinant of synaptic efficacy is the localization and density of neurotransmitter receptors in post-synaptic membrane. During the development of the neuromuscular junction (NMJ) the nicotinic acetylcholine receptor (AChR) becomes localized in high-density clusters via the motor neuron-derived protein agrin, its signaling partner MuSK, and the intracellular scaffolding protein rapsyn; however, many of the mechanisms and interactions involved in AChR localization have remained elusive. In this thesis I investigate how rapsyn interacts with the AChR, how this is regulated by agrin-induced phosphorylation of the AChR, and how this contributes to receptor localization and stability at the NMJ. First, using chimeric proteins containing an exogenous transmembrane domain and the cytoplasmic loop of the AChR subunits we demonstrate that rapsyn binds constitutively to the MA helix in the C-terminal region of the [alpha], [beta] and [varepsilon] cytoplasmic loops. Second, we determine how rapsyn also mediates agrin/MuSK-induced phosphorylation of the AChR. We show that MuSK/rapsyn expression induces parallel but independent phosphorylation of AChR [beta]Y390 and [delta]Y393, and that this is mediated by rapsyn's c-terminal domains, which localize and/or activate cytoplasmic tyrosine kinases that phosphorylate the receptor. Third, we used CD4 subunit loop chimeric proteins to screen for additional interactions that localize the AChR. Intriguingly, we find that a 20 amino acid region of the AChR-[beta] loop centered on Y390 is sufficient for postsynaptic localization, and that phosphorylation of βY390 is required for clustering. Interestingly, phosphorylation of [beta]Y390 regulates binding of the 20 amino acid motif to rapsyn and we show that this increases the stoichiometry of rapsyn to AChR. Thus, rapsyn appears to interact with the AChR via two distinct sites: constitutive binding to the MA-helix of one subunit cytoplasmic loop and regulated binding to the Y390 region of [beta] subunit loop. Fourth, we investigated whether agrin signaling - and enhanced rapsyn binding - not only clusters the AChR but also increases its metabolic stability. Indeed, we find that phosphorylation of AChR-[beta]Y390 increases receptor half-life by ~1.8 fold, which likely helps maintain functional levels of receptor at both developing and mature NMJs. Together, these findings help define the molecular interactions and regulatory mechanisms that localize nicotinic acetylcholine receptors at the NMJ, ensuring reliable neuromuscular transmission.
Drug Localization In Tissues And Cells
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Author : Walter E. Stumpf
language : en
Publisher: Iddc Press
Release Date : 2003-01-01
Drug Localization In Tissues And Cells written by Walter E. Stumpf and has been published by Iddc Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-01-01 with Medical categories.
Localization Of Putative Steroid Receptors
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Author : Louis P. Pertschuk
language : en
Publisher: CRC Press
Release Date : 2019-07-23
Localization Of Putative Steroid Receptors written by Louis P. Pertschuk and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07-23 with Medical categories.
These volumes represent the "state-of-the-art" in morphological methods for detection of steroid receptors. In Volume I we have attempted to collect current available methods and experimental approahes which might be useful in solving present enigmas. The background and recent advances presented are intended to stimulate further experimentation and new innovations, and spur clinical trials by all investigators with an interest in steroid hormones, cell biology, and clinical oncology.
Gabab Receptor
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Author : Nicole Guetg
language : en
Publisher:
Release Date : 2009
Gabab Receptor written by Nicole Guetg and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with categories.
Localization Of Putative Steroid Receptors
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Author : Pertschuk
language : en
Publisher: CRC Press
Release Date : 2018-02-01
Localization Of Putative Steroid Receptors written by Pertschuk and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-02-01 with Medical categories.
These volumes represent the "state-of-the-art" in morphological methods for detection of steroid receptors. Some physician authors have applied many of the new methodologies in clinical research projects deisgned to improve medical practice, and these attempts have been placed in Volume II. The Background and recent advances presented here are intended to stimulate further experimentation and new innovations, and spur clinical trials by all investigators with an interest in steroid hormones, cell biology, and clinical oncology.