Red Blood Cell Aggregation Characterization
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Red Blood Cell Aggregation Characterization
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Author : Rym Mehri
language : en
Publisher:
Release Date : 2016
Red Blood Cell Aggregation Characterization written by Rym Mehri and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with categories.
Red blood cells (RBCs) are the most abundant cells in human blood, representing 40 to 45% of the blood volume (hematocrit). These cells have the particular ability to deform and bridge together to form aggregates under very low shear rates. The theory and mechanics behind aggregation are, however, not yet completely understood. The purpose of this work is to provide a novel method to analyze, understand and mimic blood behaviour in microcirculation. The main objective is to develop a methodology to quantify and characterize RBC aggregates and hence enhance the current understanding of the non-Newtonian behaviour of blood at the microscale. For this purpose, suspensions of porcine blood and human blood are tested in vitro in a Poly-di-methylsiloxane (PDMS) microchannel to characterize RBC aggregates within these two types of blood. These microchannels are fabricated using standard photolithography methods. Experiments are performed using a micro Particle Image Velocimetry ( PIV) system for shear rate measurements coupled with a high speed camera for the flow visualization. Corresponding numerical simulations are conducted using a research Computational Fluid Dynamic (CFD) solver, Nek5000, based on the spectral element method solution to the incompressible non-Newtonian Navier-Stokes equations. RBC aggregate sizes are quantified in controlled and measurable shear rate environments for 5, 10 and 15% hematocrit. Aggregate sizes are determined using image processing techniques. Velocity fields of the blood flow are measured experimentally and compared to numerical simulations using simple non-Newtonian models (Power law and Carreau models). This work establishes for the first time a relationship between RBC aggregate sizes and corresponding shear rates in a microfluidic environment as well as one between RBC aggregate sizes and apparent blood viscosity at body temperature in a microfluidic controlled environment. The results of the investigation can be used to help develop new numerical models for non-Newtonian blood flow, provide a better understanding of the mechanics of RBC aggregation and help determine aggregate behaviour in clinical settings such as for degenerative diseases like diabetes and heart disease.
Physical Characterization Of Red Blood Cell Aggregation
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Author : Daniel Flormann
language : en
Publisher:
Release Date : 2017
Physical Characterization Of Red Blood Cell Aggregation written by Daniel Flormann and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
Red Blood Cell Aggregation Characterization
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Author : Curtis James Karns Armstrong
language : en
Publisher:
Release Date : 2020
Red Blood Cell Aggregation Characterization written by Curtis James Karns Armstrong and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with categories.
The presence of red blood cell (RBC) aggregation is confirmed to be a rheological phenomenon implicating abnormal physiological conditions in vivo. However, there is presently no existing technology able to analyze, characterize and detect aggregation in vivo. The Laboratoire de Mécanique et d'Acoustique (LMA, UMR 7031), Centre National de la Recherche Scientifique (CNRS) at Aix-Marseille Universite (AMU) is developing a technology to measure blood aggregation in vivo using ultrasound backscattering techniques. In doing so it aims to allow disease prevention and disease recognition. The methodology developed at LMA is currently being compared to previous methodologies used to quantify RBC aggregation. Further study is needed to compare the methodologies used in LMA to microscopic imaging techniques, which are considered the gold standard in aggregation characterization. This thesis focuses on the development of a microfluidic device dedicated to the visualization of RBC aggregation. The device is capable of low compliance to ensure repeatability of the flow rate, with good optical clarity. The device's co-flow properties and capabilities were tested and analyzed to ensure a proper comparison of methodologies could be conducted. Once completed, the incorporation of an ultrasound transducer to the setup, will be done in France to directly compare the methodologies developed by LMA and confirm the models derived by Franceschini et al. In order to develop the microfluidic chip, this thesis considers an overview of the methodology for characterizing RBC aggregation, the fabrication and compliant verification of a novel Norland Optical Adhesive microfluidic chip, and the shear rate calibration of the microfluidic device using Dextran 70 and Dextran 500. The NOA63 microfluidic device was calibrated to flow factors based on a shearing flow ratio of 25:1. The Norland Optical Adhesive microfluidic device had a much lower level of compliance in comparison to the gold standard of PDMS. The NOA63 device was found to be 51% less compliant than its counter part of PDMS. The device was calibrated to control shear rates from around 60s-1 to 0.01s-1. Multiple concentrations of Dextran 70 and Dextran 500 in human blood samples at 10% hematocrit were tested to characterize the shear rate in the blood layer. Aggregates were found to align themselves parallel to the flow and were observed to haven anisotropic shapes. All results from this thesis are being used to support the development of an ultrasound device capable of measuring blood aggregation in vivo.
Red Blood Cell Aggregation
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Author : Oguz Baskurt
language : en
Publisher: CRC Press
Release Date : 2011-09-28
Red Blood Cell Aggregation written by Oguz Baskurt and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-28 with Medical categories.
Red blood cells in humans-and most other mammals-have a tendency to form aggregates with a characteristic face-to-face morphology, similar to a stack of coins. Known as rouleaux, these aggregates are a normally occurring phenomenon and have a major impact on blood rheology. What is the underlying mechanism that produces this pattern? Does this real
Characterization Of Red Blood Cell Aggregation Dynamics With Doppler Ultrasound
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Author : Zhao Qin
language : en
Publisher:
Release Date : 1997
Characterization Of Red Blood Cell Aggregation Dynamics With Doppler Ultrasound written by Zhao Qin and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with categories.
Red Blood Cell Aggregation Rheological And Microstructural Characteristics
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Author : Efstathios C. Kaliviotis
language : en
Publisher:
Release Date : 2009
Red Blood Cell Aggregation Rheological And Microstructural Characteristics written by Efstathios C. Kaliviotis and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with Erythrocytes categories.
Preparation Characterization And Rheological Properties Of Star Shaped Poly Ethylene Glycol With A Cholane Core And Study Of Its Effect On Red Blood Cell Aggregation
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Author : Florence Janvier
language : en
Publisher:
Release Date : 2009
Preparation Characterization And Rheological Properties Of Star Shaped Poly Ethylene Glycol With A Cholane Core And Study Of Its Effect On Red Blood Cell Aggregation written by Florence Janvier and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with categories.
Red Blood Cells At The Mount Of Truth Highlights Of The 22nd Meeting Of The European Red Cell Research Society
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Author : Anna Bogdanova
language : en
Publisher: Frontiers Media SA
Release Date : 2021-01-06
Red Blood Cells At The Mount Of Truth Highlights Of The 22nd Meeting Of The European Red Cell Research Society written by Anna Bogdanova and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-01-06 with Science categories.
Bioengineering In Cell And Tissue Research
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Author : Gerhard M. Artmann
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-04-09
Bioengineering In Cell And Tissue Research written by Gerhard M. Artmann and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-04-09 with Technology & Engineering categories.
Cutting edge research in cell and tissue research abounds in this review of the latest technological developments in the area. The chapters are written by excellent scientists on advanced, frontier technology and address scientific questions that require considerable engineering brainpower. The aim is to provide students and scientists working in academia and industry new information on bioengineering in cell and tissue research to enhance their understanding and innovation.
Red Blood Cell Aggregation
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Author : Oguz Baskurt
language : en
Publisher: CRC Press
Release Date : 2011-09-28
Red Blood Cell Aggregation written by Oguz Baskurt and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-28 with Medical categories.
Red blood cells in humans—and most other mammals—have a tendency to form aggregates with a characteristic face-to-face morphology, similar to a stack of coins. Known as rouleaux, these aggregates are a normally occurring phenomenon and have a major impact on blood rheology. What is the underlying mechanism that produces this pattern? Does this really happen in blood circulation? And do these rouleaux formations have a useful function? The first book to offer a comprehensive review of the subject, Red Blood Cell Aggregation tackles these and other questions related to red blood cell (RBC) aggregates. The book covers basic, clinical, and physiological aspects of this important biophysical phenomenon and integrates these areas with concepts in bioengineering. It brings together state-of-the-art research on the determinants, mechanisms, and measurement and effects of RBC aggregation as well as on variations and comparative aspects. After an introductory overview, the book outlines factors and conditions that affect RBC aggregation. It presents the two hypotheses—the bridging model and the depletion model—that provide potential mechanisms for the adhesive forces that lead to the regular packing of the cells in rouleaux formations. The book also reviews the methods used to quantify RBC aggregation in vitro, focusing on their importance in clinical practice. Chapters discuss the effect of RBC aggregation on the in vitro rheology of blood as well as on tube flow. The book also looks at what happens in the circulation when red blood cells aggregate and examines variations due to physiological and pathophysiological challenges. The concluding chapter explores the formation of red blood cell aggregates in other mammals. Written by leading researchers in the field, this is an invaluable resource for basic science, medical, and clinical researchers; graduate students; and clinicians interested in mammalian red blood cells.