Redox Control Of The Plant Specific Cap Binding Protein Eifiso4e In Arabidopsis
DOWNLOAD
Download Redox Control Of The Plant Specific Cap Binding Protein Eifiso4e In Arabidopsis PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Redox Control Of The Plant Specific Cap Binding Protein Eifiso4e In Arabidopsis book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Redox Control Of The Plant Specific Cap Binding Protein Eifiso4e In Arabidopsis
DOWNLOAD
Author : Ching-Ying Tseng (Ph. D.)
language : en
Publisher:
Release Date : 2015
Redox Control Of The Plant Specific Cap Binding Protein Eifiso4e In Arabidopsis written by Ching-Ying Tseng (Ph. D.) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
Eukaryotic initiation factor (eIF) 4E and the plant-specific homolog, eIFiso4E, participate in translation initiation by recognizing the 7‐methylguanosine (m7G) cap of mRNAs. All plant cap-binding proteins (eIF4E and eIFiso4E) have two conserved cysteine residues and wheat (Triticum aestivum) eIF4E was observed in crystals to form a disulfide bond between these two residues under oxidizing conditions. To study the effect of these conserved cysteines on Arabidopsis thaliana eIFiso4E activity in vivo or in vitro, one of the conserved residues (Cys-97) was mutated to serine (AteIFiso4E C97S), which abolishes the ability to form a disulfide bond or undergo modification. Under abiotic stress, AteIFiso4E C97S plants showed stress hypersensitivity and slowed ROS (reactive oxygen species) propagation, which suggests an in vivo role of Cys-97 in response to stress induced redox. The in vitro cap binding activity of AteIFiso4E C97S and in vitro translation activity of AteIFiso4F C97S (the complex of eIFiso4E C97S and eIFiso4G) was measured. AteIFiso4E C97S is not able to bind m7GTP-Sepharose affinity resin, but its binding can be restored by interaction with its binding partner, AteIFiso4G. However, a capped mRNA (barley [alpha]-amylase) was translated in vitro at a 3-fold lower rate by AteIFiso4F C97S compared to AteIFiso4F. These findings suggest that cysteine modification of AteIFiso4E and similarly conserved cysteine residues of other plant cap binding proteins (eIF4E) may have a role in regulation of translation during responses to the environment or other conditions that may alter the cellular redox status. To create a simple in vivo model for studying the differential activities of AteIF4E and AteIFiso4E or their complexes (AteIF4F and AteIFiso4F) towards messenger RNA (mRNA), yeast strains were created to attempt to substitute yeast eIF4E or eIF4G or both for Arabidopsis subunits. Lastly, an Arabidopsis mutant for ascorbic acid peroxidase (apx1) accumulates a high amount of cytosolic H2O2 which generates a more oxidizing environment. Arabidopsis carrying only exogenous AteIFiso4E or AteIFiso4E C97S in the eifiso4e/eif4e1/apx1 triple knockout were characterized for the response to this highly oxidizing environment.
The Roles Of Alternative Cap Binding Proteins Of Arabidopsis Thaliana
DOWNLOAD
Author : Ryan Michael Patrick
language : en
Publisher:
Release Date : 2015
The Roles Of Alternative Cap Binding Proteins Of Arabidopsis Thaliana written by Ryan Michael Patrick and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
The mRNA cap-binding complexes eIF4F (made up of the cap-binding protein eIF4E and the large scaffold eIF4G) and eIFiso4F (made up of the plant-specific isoforms eIFiso4E and eIFiso4G) have established roles in translation initiation. However, other cap-binding proteins are known to be encoded in the Arabidopsis thaliana genome. We have chosen to investigate the biochemical properties and potential functions of these proteins. We have identified the eIF4E-like proteins, eIF4E1b and eIF4E1c, as Brassicaceae-specific eIF4E isoforms with the ability to form cap-binding complexes. These proteins are able to complement an eIF4E deletion in yeast. However, their limited expression in A. thaliana along with their relatively weak binding affinity for eIF4G and more limited ability to promote translation in vitro indicate a possible role outside of canonical translation initiation pathways. The alternative cap-binding protein 4EHP is conserved from animals to plants, but its role and binding partners in plants are not well defined. We demonstrate that a homologous complex to the 4EHP-GIGYF2 cap-binding complex observed in mammals is present in A. thaliana. The plant complex appears important to proper development, as double knockouts show a noticeable developmental phenotype and dysregulation of gene expression, but the viability of these knockouts in A. thaliana may offer an opportunity to research the complex's function that cannot be performed in animal systems as knockouts are lethal. RNA immunoprecipitation studies find that 4EHP and GIGYF associate with non-coding RNA in A. thaliana, and nucleocytoplasmic fractionation supports a possible nuclear role for the proteins. These findings indicate that the 4EHP-GIGYF complex may have an unexpected role in bridging non-coding RNA to gene expression in plants.
Evolution Of The Protein Synthesis Machinery And Its Regulation
DOWNLOAD
Author : Greco Hernández
language : en
Publisher: Springer
Release Date : 2016-08-10
Evolution Of The Protein Synthesis Machinery And Its Regulation written by Greco Hernández and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-10 with Science categories.
The “omics” era has given a new perspective to the findings on the origin and evolution of the process of translation. This book provides insight into the evolution of the translation process and machinery from a modern perspective. Written by leading experts in molecular biology, this text looks into the origins and evolution of the protein synthetic machinery.
Rna Exosome
DOWNLOAD
Author : Torben Heick Jensen
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-06-29
Rna Exosome written by Torben Heick Jensen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-29 with Medical categories.
The diversity of RNAs inside living cells is amazing. We have known of the more “classic” RNA species: mRNA, tRNA, rRNA, snRNA and snoRNA for some time now, but in a steady stream new types of molecules are being described as it is becoming clear that most of the genomic information of cells ends up in RNA. To deal with the enormous load of resulting RNA processing and degradation reactions, cells need adequate and efficient molecular machines. The RNA exosome is arising as a major facilitator to this effect. Structural and functional data gathered over the last decade have illustrated the biochemical importance of this multimeric complex and its many co-factors, revealing its enormous regulatory power. By gathering some of the most prominent researchers in the exosome field, it is the aim of this volume to introduce this fascinating protein complex as well as to give a timely and rich account of its many functions. The exosome was discovered more than a decade ago by Phil Mitchell and David Tollervey by its ability to trim the 3’end of yeast, S. cerevisiae, 5. 8S rRNA. In a historic account they laid out the events surrounding this identification and the subsequent birth of the research field. In the chapter by Kurt Januszyk and Christopher Lima the structural organization of eukaryotic exosomes and their evolutionary counterparts in bacteria and archaea are discussed in large part through presentation of structures.
Genomic Designing Of Climate Smart Vegetable Crops
DOWNLOAD
Author : Chittaranjan Kole
language : en
Publisher: Springer Nature
Release Date : 2020-03-02
Genomic Designing Of Climate Smart Vegetable Crops written by Chittaranjan Kole and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-03-02 with Science categories.
This book reviews modern strategies in the breeding of vegetables in the era of global warming. Agriculture is facing numerous challenges in the 21st century, as it has to address food, nutritional, energy and environmental security. Future vegetable varieties must be adaptive to the varying scenarios of climate change, produce higher yields of high- quality food and feed and have multiple uses. To achieve these goals, it is imperative to employ modern tools of molecular breeding, genetic engineering and genomics for ‘precise’ plant breeding to produce ‘designed’ vegetable varieties adaptive to climate change. This book is of interest to scientists working in the fields of plant genetics, genomics, breeding, biotechnology, and in the disciplines of agronomy and horticulture.
Molecular Mechanism Of Crucifer S Host Resistance
DOWNLOAD
Author : Govind Singh Saharan
language : en
Publisher: Springer Nature
Release Date : 2022-01-03
Molecular Mechanism Of Crucifer S Host Resistance written by Govind Singh Saharan and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-03 with Science categories.
The book is a comprehensive compilation of applied knowledge for developing resistant varieties to all the major biotrophs, hemibiotrophs and necrotrophs pathogens of crucifers through the use of latest biotechnological approaches. The book includes, multi-component resistance, incorporation of non-host resistance gene, function of particular gene in resistance, expression of age related resistance, enhanced gene resistance, sources of alternative gene which enhance disease resistance, through the use of latest biotechnical approaches like proteomics, omics, transcriptomics and metabolomics. The book also explores the molecular basis of disease resistance, its biometabolomics activities in response to infection and interaction by the various biotrophs, hemibiotrophs and necrotrophs pathogens. The identification of R genes and its incorporation into agronomically superior varieties through use of molecular mechanisms is also explained. This compilation is immensely useful to the researchers especially Brassica breeders, teachers, extension specialists, students, industrialists, farmers, and all others who are interested to grow healthy, and profitable cruciferous crops all over the world.
Biotic And Abiotic Stress Tolerance In Plants
DOWNLOAD
Author : Sharad Vats
language : en
Publisher: Springer
Release Date : 2018-06-11
Biotic And Abiotic Stress Tolerance In Plants written by Sharad Vats and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-06-11 with Science categories.
This book highlights some of the most important biochemical, physiological and molecular aspects of plant stress, together with the latest updates. It is divided into 14 chapters, written by eminent experts from around the globe and highlighting the effects of plant stress (biotic and abiotic) on the photosynthetic apparatus, metabolites, programmed cell death, germination etc. In turn, the role of beneficial elements, glutathione-S-transferase, phosphite and nitric oxide in the adaptive response of plants under stress and as a stimulator of better plant performance is also discussed. A dedicated chapter addresses research advances in connection with Capsicum, a commercially important plant, and stress tolerance, from classical breeding to the recent use of large-scale transcriptome and genome sequencing technologies. The book also explores the significance of the liliputians of the plant kingdom (Bryophytes) as biomonitors/bioindicators, and general and specialized bioinformatics resources that can benefit anyone working in the field of plant stress biology. Given the information compiled here, the book will offer a valuable guide for students and researchers of plant molecular biology and stress physiology alike.
Plant Resistance To Viruses
DOWNLOAD
Author : David Evered
language : en
Publisher: John Wiley & Sons
Release Date : 2008-04-30
Plant Resistance To Viruses written by David Evered and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-04-30 with Science categories.
Concern about the environmental consequences of the widespread use of pesticides has increased, and evidence of pesticide-resistant virus vectors have continued to emerge. This volume presents a timely survey of the mechanisms of plant resistance and examines current developments in breeding for resistance, with particular emphasis on advances in genetic engineering which allow for the incorporation of viral genetic material into plants. Discusses the mechanisms of innate resistance in strains of tobacco, tomato, and cowpea; various aspects of induced resistance, including the characterization and roles of the pathogenesis-related proteins; antiviral substances and their comparison with interferon; and cross-protection between plant virus strains. Also presents several papers which evaluate the status of genetic engineering as it relates to breeding resistant plants. Among these are discussions of the potential use of plant viruses as gene vectors, gene coding for viral coat protein, satellite RNA, and antisense RNA, and practical issues such as the durability of resistant crop plants in the field.
The Capsicum Genome
DOWNLOAD
Author : Nirala Ramchiary
language : en
Publisher: Springer
Release Date : 2019-02-18
The Capsicum Genome written by Nirala Ramchiary and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-02-18 with Science categories.
This book contains complete information on Capsicum genetic resources, diversity, evolution, history and advances in capsicum improvement from classical breeding to whole genome sequencing, genomics, databases and its impact on next generation pepper breeding. Capsicum is one of the most important Solanaceae crops grown worldwide as vegetables and spices. Due to its high economic value and to meet the demands of enormous population growth amid biotic and abiotic stresses, there has been an ongoing breeding program utilizing available genetic resources with desired traits to increase the sustainable productivity of this crop for several decades. However, the precision breeding of this crop for desired traits only started with the advent of molecular markers. The recent advances in high-throughput genome sequencing technologies helped in the quick decoding of transcriptome, epigenome, nuclear and organeller genomes, thereby enhancing our understanding of the structure and function of the Capsicum genome, and helping in genomics assisted breeding. These advanced technologies coupled with conventional mapping have greatly contributed towards dissection and manipulation of economically important traits more precisely and made less time consuming.
Protein Kinase Ck2 Cellular Function In Normal And Disease States
DOWNLOAD
Author : Khalil Ahmed
language : en
Publisher: Springer
Release Date : 2015-03-03
Protein Kinase Ck2 Cellular Function In Normal And Disease States written by Khalil Ahmed and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-03-03 with Medical categories.
Protein kinase CK2 (formerly casein kinase II or 2) is known to play a critical role in the control of cell growth and cell death and is thus intimately involved in the development of cancer. More specifically, CK2 has been found to be elevated in all cancers examined. While CK2 levels are known to be high in proliferating normal cells, CK2 has also been found to be a potent suppressor of apoptosis and is a link to the cancer cell phenotype, which is characterized by deregulation of both cell proliferation and cell death. Indeed, it would appear that CK2 impacts many of the hallmarks of cancer and it has now gained considerable attention as a potential target for cancer therapy. Protein Kinase CK2 and Cellular Function in Normal and Disease States increases knowledge of the role of CK2 in the development of cellular dysfunction and emphasizes that this protein may serve as a target of drug development for improved cancer therapy. In addition, it is a handy tool that provides cancer researchers, graduate students, and all scientists involved in CK2 research with one main source for the latest advances in CK2 research.