The Roles Of Alternative Cap Binding Proteins Of Arabidopsis Thaliana
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The Roles Of Alternative Cap Binding Proteins Of Arabidopsis Thaliana
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Author : Ryan Michael Patrick
language : en
Publisher:
Release Date : 2015
The Roles Of Alternative Cap Binding Proteins Of Arabidopsis Thaliana written by Ryan Michael Patrick and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
The mRNA cap-binding complexes eIF4F (made up of the cap-binding protein eIF4E and the large scaffold eIF4G) and eIFiso4F (made up of the plant-specific isoforms eIFiso4E and eIFiso4G) have established roles in translation initiation. However, other cap-binding proteins are known to be encoded in the Arabidopsis thaliana genome. We have chosen to investigate the biochemical properties and potential functions of these proteins. We have identified the eIF4E-like proteins, eIF4E1b and eIF4E1c, as Brassicaceae-specific eIF4E isoforms with the ability to form cap-binding complexes. These proteins are able to complement an eIF4E deletion in yeast. However, their limited expression in A. thaliana along with their relatively weak binding affinity for eIF4G and more limited ability to promote translation in vitro indicate a possible role outside of canonical translation initiation pathways. The alternative cap-binding protein 4EHP is conserved from animals to plants, but its role and binding partners in plants are not well defined. We demonstrate that a homologous complex to the 4EHP-GIGYF2 cap-binding complex observed in mammals is present in A. thaliana. The plant complex appears important to proper development, as double knockouts show a noticeable developmental phenotype and dysregulation of gene expression, but the viability of these knockouts in A. thaliana may offer an opportunity to research the complex's function that cannot be performed in animal systems as knockouts are lethal. RNA immunoprecipitation studies find that 4EHP and GIGYF associate with non-coding RNA in A. thaliana, and nucleocytoplasmic fractionation supports a possible nuclear role for the proteins. These findings indicate that the 4EHP-GIGYF complex may have an unexpected role in bridging non-coding RNA to gene expression in plants.
Identifying The Role Of The Cap Binding Complexes In The Regulation Of Translation In Arabidopsis Thaliana
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Author : Nicola Ann Cole
language : en
Publisher:
Release Date : 2017
Identifying The Role Of The Cap Binding Complexes In The Regulation Of Translation In Arabidopsis Thaliana written by Nicola Ann Cole and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
There is a fundamental gap in our understanding of the regulation of translation in plants. None of the canonical eukaryotic methods found in mammals, such as the use of 4E-Binding protein phosphorylation and inhibition of eIF2B function by phosphorylation of eIF2[alpha], have been found in plants to date. In the very early steps of eukaryotic initiation of translation, the cap-binding complex, eIF4F, binds to the m7G of the mRNA and positions it for binding by the 43S preinitiation complex. This complex consists of the large scaffolding protein, eIF4G, and the smaller cap-binding protein, eIF4E, along with the DEAD box helicase eIF4A and the RNA binding protein eIF4B. In addition to eIF4F, plants have a well-conserved second cap-binding complex, eIFiso4F, not found in other eukaryotes and comprised of eIFiso4G and eIFiso4E, isoforms of the scaffolding protein and the cap-binding protein, respectively. Arabidopsis thaliana plants lacking eIFiso4F have stunted growth, reduced chlorophyll, reduced fertility and are more sensitive to light stress. In in vitro translation assays, there is a preference for eIF4F by capped messages. In this work, several possible methods for the regulation of translation in Arabidopsis thaliana by eIF4F and eIFiso4F are investigated. A previously discovered eIF4G wheat kinase known to phosphorylate eIF4G, eIFiso4G, eIF4B and a 60S ribosomal protein is further characterized to determine its possible role in the regulation of translation. It is found to be a calcium dependent kinase that phosphorylates eIFiso4G in the C-terminal domain most likely at a consensus sequence for calcium dependent protein kinases. Additionally, there is a conserved pair of cysteines located in the cap-binding pocket of both eIF4E and eIFiso4E, found only in plants. In an in vitro labeling assay, these cysteines are tested for their thiol reactivity in eIF4E versus eIFiso4E. eIFiso4E is found to be more thiol reactive than eIF4E, possibly indicating differences in redox activity between the two proteins. Finally, two methods for identifying specific populations of mRNA associated with plant protein synthesis initiation factors eIF4F and eIFiso4F are described, RNA immunoprecipitation and ribosome profiling.
Rna Protein Interactions
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Author : Kiyoshi Nagai
language : en
Publisher: Oxford University Press, USA
Release Date : 1994
Rna Protein Interactions written by Kiyoshi Nagai and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.
The study of RNA-protein interactions is crucial to understanding the mechanisms and control of gene expression and protein synthesis. The realization that RNAs are often far more biologically active than was previously appreciated has stimulated a great deal of new research in this field. Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural biology.
Nuclear Pre Mrna Processing In Plants
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Author : A. S. N. Reddy
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-04-16
Nuclear Pre Mrna Processing In Plants written by A. S. N. Reddy and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-04-16 with Science categories.
During the last few years, tremendous progress has been made in understanding various aspects of pre-mRNA processing. This book, with contributions from leading scientists in this area, summarizes recent advances in nuclear pre-mRNA processing in plants. It provides researchers in the field, as well as those in related areas, with an up-to-date and comprehensive, yet concise, overview of the current status and future potential of this research in understanding plant biology.
Rna Binding Proteins Advances In Research And Application 2011 Edition
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Author :
language : en
Publisher: ScholarlyEditions
Release Date : 2012-01-09
Rna Binding Proteins Advances In Research And Application 2011 Edition written by and has been published by ScholarlyEditions this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-01-09 with Medical categories.
RNA-Binding Proteins: Advances in Research and Application: 2011 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about RNA-Binding Proteins in a concise format. The editors have built RNA-Binding Proteins: Advances in Research and Application: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about RNA-Binding Proteins in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of RNA-Binding Proteins: Advances in Research and Application: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Alternative Splicing Regulation In Plants
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Author : Ezequiel Petrillo
language : en
Publisher: Frontiers Media SA
Release Date : 2020-09-02
Alternative Splicing Regulation In Plants written by Ezequiel Petrillo and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-09-02 with Nature categories.
This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.
Plant Micrornas
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Author : Stefan de Folter
language : en
Publisher: Humana Press
Release Date : 2019-01-31
Plant Micrornas written by Stefan de Folter and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-01-31 with Science categories.
This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science.
Issues In Biochemistry And Biophysics Research 2011 Edition
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Author :
language : en
Publisher: ScholarlyEditions
Release Date : 2012-01-09
Issues In Biochemistry And Biophysics Research 2011 Edition written by and has been published by ScholarlyEditions this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-01-09 with Science categories.
Issues in Biochemistry and Biophysics Research: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Biochemistry and Biophysics Research. The editors have built Issues in Biochemistry and Biophysics Research: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Biochemistry and Biophysics Research in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Biochemistry and Biophysics Research: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Evolution Of The Protein Synthesis Machinery And Its Regulation
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Author : Greco Hernández
language : en
Publisher: Springer
Release Date : 2016-08-10
Evolution Of The Protein Synthesis Machinery And Its Regulation written by Greco Hernández and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-10 with Science categories.
The “omics” era has given a new perspective to the findings on the origin and evolution of the process of translation. This book provides insight into the evolution of the translation process and machinery from a modern perspective. Written by leading experts in molecular biology, this text looks into the origins and evolution of the protein synthetic machinery.
Elsevier S Integrated Review Biochemistry
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Author : John W. Pelley, PhD
language : en
Publisher: Elsevier Health Sciences
Release Date : 2011-11-30
Elsevier S Integrated Review Biochemistry written by John W. Pelley, PhD and has been published by Elsevier Health Sciences this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-11-30 with Medical categories.
Effectively merge basic science and clinical skills with Elsevier's Integrated Review Biochemistry, by John W. Pelley, PhD. This concise, high-yield title in the popular Integrated Review Series focuses on the core knowledge in biochemistry while linking that information to related concepts from other basic science disciplines. Case-based questions at the end of each chapter enable you to gauge your mastery of the material, and a color-coded format allows you to quickly find the specific guidance you need. Online access via www.studentconsult.com - included with your purchase - allows you to conveniently access the book's complete text and illustrations online as well as relevant content from other Student Consult titles. This concise and user-friendly reference provides crucial guidance for the early years of medical training and USMLE preparation. Spend more time reviewing and less time searching thanks to an extremely focused, "high-yield" presentation. Gauge your mastery of the material and build confidence with both case-based, andUSMLE-style questions that provide effective chapter review and quick practice for your exams. Access the full contents online at www.studentconsult.com where you'll find the complete text and illustrations, "Integration Links" to bonus content in other Student Consult titles, an interactive community center with a wealth of additional resources, and much more! Grasp and retain vital concepts more easily thanks to a color-coded format, succinct,text, key concept boxes, and dynamic illustrations that facilitate learning in a highly visual approach. Effectively review for problem-based courses with the help of text boxes that help you clearly see the clinical relevance of the material. Great for visual learners!