Resin Microscopy And On Section Immunocytochemistry
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Resin Microscopy And On Section Immunocytochemistry
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Author : Geoffrey R. Newman
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Resin Microscopy And On Section Immunocytochemistry written by Geoffrey R. Newman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Since antibodies tagged with markers have been developed, immunocytochemistry has become an important method for identifying tissue substances and the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the techniques can be acquired.
Microscopy Immunohistochemistry And Antigen Retrieval Methods
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Author : M.A. Hayat
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-05-08
Microscopy Immunohistochemistry And Antigen Retrieval Methods written by M.A. Hayat and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-05-08 with Medical categories.
Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.
Autoradiography And Immunocytochemistry
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Author : M.A. Williams
language : en
Publisher: Elsevier
Release Date : 2013-10-22
Autoradiography And Immunocytochemistry written by M.A. Williams and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-10-22 with Science categories.
Autoradiography and Immunocytochemistry sets out in detail the preparation of autoradiographs and methods of preparing and use of immunological staining reagents. This book begins with an introduction to the demand for autoradiography and immunocytochemistry and their most advantageous use, followed by a discussion on the immunocytochemistry at the electron microscopical (EM) level in relation to the preparation of labeled antibodies. Other topics include the general remarks on “staining with antibodies; applying Fer-Ab conjugates to ultrathin sections; and controls in immunoferritin staining. The multistep antibody staining methods with unmodified proteins; control observations in staining experiments using lectins; and pattern analysis in EM immunocytochemistry are also covered. This text concludes with a description of the preparation of electron microscope autoradiographs, including the radioisotopes in EM autoradiography, preparation of radioactively-labeled tissues and cells, and methods of applying nuclear emulsions. This publication serves as a detailed laboratory guide for researchers and workers to successfully conduct electron microscope techniques.
Immunohistochemistry And Immunocytochemistry
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Author : Simon Renshaw
language : en
Publisher: John Wiley & Sons
Release Date : 2017-02-15
Immunohistochemistry And Immunocytochemistry written by Simon Renshaw and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-02-15 with Science categories.
Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.
Electron Microscopic Immunocytochemistry
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Author : Julia M. Polak
language : en
Publisher: Oxford University Press, USA
Release Date : 1992
Electron Microscopic Immunocytochemistry written by Julia M. Polak and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Medical categories.
Electron microscopic immunocytochemistry is a powerful technique combining the resolution of the electron microscope with the specificity and sensitivity of immunocytochemistry. This book discusses methods designed to make this intricate procedure more accessible, and covers recent developments that have simplified the technique and broadened the range of applications. It presents the principles of EM immunocytochemistry, detailed protocols, and examples of ways in which the technique can be applied to a variety of fields, including biomedical research and diagnostic pathology. The book begins with an overview of EM immunocytochemical procedures and of applications in histopathology. The three major chapters cover post-embedding, pre-embedding, and ultra-thin cryosection techniques. The final section illustrates applications of EM immunocytochemistry to the study of endocrine tumors, renal disease, neuropathology, dermatology, and microbiology. The emphasis throughout this detailed volume is on providing practical guidance to scientists and clinicians who may be using the technique for the first time.
Immunocytochemistry
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Author : Julian E. Beesley
language : en
Publisher: Oxford University Press, USA
Release Date : 1993
Immunocytochemistry written by Julian E. Beesley and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Medical categories.
Immunocytochemical techniques are now widely employed in research and diagnostic laboratories, enabling the identification, and often the quantification, of specific antigens present in a biological sample.
An Introduction To Immunocytochemistry
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Author : Julia M. Polak
language : en
Publisher: Oxford University Press, USA
Release Date : 1987
An Introduction To Immunocytochemistry written by Julia M. Polak and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Cytochemistry categories.
This work contains a concise exposition of the different immunocytochemical methods for identifying tissue constituents, discussing ways of preparing tissues according to the antigen to be localized and the most commonly used methods. The text is illustrated with explanatory diagrams and photomicrographs. An appendix contains step-by-step instructions for many of the basic techniques at light and electron microscopical levels. Other sections deal with sources of error and nonspecificity, and techniques for recording results photographically. For this revised edition, the authors have updated the text in light of the latest developments and added 20 new references to the literature.
Immunocytochemistry And In Situ Hybridization In The Biomedical Sciences
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Author : Julian E. Beesley
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Immunocytochemistry And In Situ Hybridization In The Biomedical Sciences written by Julian E. Beesley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.
Biological Specimen Preparation For Transmission Electron Microscopy
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Author : Audrey M. Glauert
language : en
Publisher: Princeton University Press
Release Date : 2014-07-14
Biological Specimen Preparation For Transmission Electron Microscopy written by Audrey M. Glauert and has been published by Princeton University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-14 with Science categories.
This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
Introduction To Immunocytochemistry
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Author : Julia M. Polak
language : en
Publisher: Taylor & Francis
Release Date : 1997
Introduction To Immunocytochemistry written by Julia M. Polak and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.
Immunocytochemical techniques are essential in diagnosis and biomedical research. This rewritten and updated introduction for beginners explains the reasons for the various steps, and discusses problems for specificity and sensitivity and their solutions. It includes practical instructions for several immunostaining methods using flourescent, enzyme and gold labels.