Resolution And Localization In Single Molecule Microscopy
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Resolution And Localization In Single Molecule Microscopy
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Author : Sripad Ram
language : en
Publisher: ProQuest
Release Date : 2007
Resolution And Localization In Single Molecule Microscopy written by Sripad Ram and has been published by ProQuest this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Biomedical engineering categories.
Rayleigh's criterion is extensively used in optical microscopy to determine the resolution of microscopes. Despite its widespread use, it is well known that this criterion is based on heuristic notions and can be surpassed in a regular optical microscope. The inadequacy of Rayleigh's criterion has necessitated a reassessment of the resolution limits of optical microscopes. The thesis proposes a new resolution criterion that overcomes the limitations of Rayleigh's criterion. The new result predicts that there is no resolution limit, but that the resolvability depends on the number of detected photons. Analytical tools are introduced to estimate the distance from microscopy images. By imaging fluorescently labeled DNA nano-rulers, it is shown that distances as small as 12 nm can be measured from experimental data with accuracy as predicted by the new resolution criterion. The new result is derived by adopting a stochastic framework and using the theory concerning the Fisher information matrix. This approach is generalized to a wide variety of estimation problems in optical microscopy by deriving expressions for the limits to the accuracy of the parameter estimates. As an application, the thesis addresses the location estimation problem. Analytical formulae are derived that provide a limit to the accuracy with which the location of a microscopic object can be determined. These results are illustrated by considering specific image profiles that describe the image of a single molecule. Another contribution of this thesis is the development of a new microscopy technique called multifocal plane microscopy for tracking single molecules/particles in 3D. An important property of this technique is its improved depth discrimination capability, which in turn enables accurate determination of the axial location of the particle especially when it is close to the plane of focus.
Single Molecule Image Analysis
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Author : Christian Franke
language : en
Publisher: Frontiers Media SA
Release Date : 2022-12-15
Single Molecule Image Analysis written by Christian Franke and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-12-15 with Science categories.
Super Resolution Microscopy For Material Science
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Author : Lorenzo Albertazzi
language : en
Publisher: CRC Press
Release Date : 2024-03-26
Super Resolution Microscopy For Material Science written by Lorenzo Albertazzi and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-03-26 with Science categories.
Optical microscopy is one of the most frequently used tools in chemistry and the life sciences. However, its limited resolution hampers the use of optical imaging to many other relevant problems in different disciplines. Super-Resolution Microscopy (SRM) is a new technique that allows the resolution of objects down to a few billionth of meters (nanometers), ten times better than classical microscopes, opening up opportunities to use this tool in new fields. This book describes the theory, principles, and practice of super-resolution microscopy in the field of materials science and nanotechnology. There is a growing interest in the applications of SRM beyond biology as new synthetic materials, such as nanoscale sensors and catalysts, nanostructured materials, functional polymers, and nanoparticles, have nanoscopic features that are challenging to visualize with traditional imaging methods. SRM has the potential to be used to image and understand these cutting-edge man-made objects and guide the design of materials for novel applications. This book is an ideal guide for researchers in the fields of microscopy and materials science and chemistry as well as graduate students studying physics, materials science, biomedical engineering, and chemistry. Key Features: Contains practical guidance on Super-Resolution Microscopy (SRM), an exciting and growing tool that was awarded the Nobel Prize for chemistry in 2014 Provides a new perspective targeting materials science, unlike existing books which target readers in chemistry, life science, and biology Targets students in its core chapters, while offering more advanced material for professionals and researchers in later chapters
Super Resolution Microscopy
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Author : Holger Erfle
language : en
Publisher:
Release Date : 2018-08-13
Super Resolution Microscopy written by Holger Erfle and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-08-13 with categories.
Super Resolution Microscopy
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Author : Udo J. Birk
language : en
Publisher: John Wiley & Sons
Release Date : 2017-12-04
Super Resolution Microscopy written by Udo J. Birk and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-04 with Science categories.
This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.
Super Resolution Optical Imaging And Microscopy
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Author : Junle Qu
language : en
Publisher: John Wiley & Sons
Release Date : 2023-12-11
Super Resolution Optical Imaging And Microscopy written by Junle Qu and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-12-11 with Science categories.
Super Resolution Optical Imaging and Microscopy Extremely comprehensive resource containing cutting-edge and practical knowledge of super-resolution optical imaging This book covers both the basic principles and specific technical details of super-resolution microscopy techniques. It covers the criteria to choose different fluorophores for various SRM methods and critically assesses the nitty-gritty of associated problems that are often encountered in practical applications. A progressive guide to designing the next generation of advanced fluorophores to meet the goal of advanced SR imaging studies is also put forward. Written by two well-qualified authors, the book contains exclusive content to enhance readers’ understanding on innovation of newer SRM technologies. Sample topics covered in the book include: Optical techniques, fluorescent probe design, and algorithm development Recent highlight and breakthroughs in biology using SRM methods The overall success of SRM in biological inventions The future direction and scope of the field This book is an invaluable resource for chemists and researchers/scientists involved in designing newer fluorescent materials for SRM studies. It can also assist biologists engaged in advanced biological studies using SRM by guiding them through sample preparation, image processing, and precautions to be taken in practical imaging studies.
Single Molecule Science
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Author : Krishnarao Appasani
language : en
Publisher: Cambridge University Press
Release Date : 2022-05-26
Single Molecule Science written by Krishnarao Appasani and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-05-26 with Medical categories.
A comprehensive volume that brings together authoritative overviews of single molecule science techniques from a biological perspective.
Super Resolution Imaging In Biomedicine
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Author : Alberto Diaspro
language : en
Publisher: CRC Press
Release Date : 2016-11-03
Super Resolution Imaging In Biomedicine written by Alberto Diaspro and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-11-03 with Science categories.
This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.
Single Molecule Spectroscopy
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Author : R. Rigler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Single Molecule Spectroscopy written by R. Rigler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.
A Self Assembly Approach To Localization And Patterning Of Optically Resolved Single Molecules
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Author : Randall Mark Stoltenberg
language : en
Publisher: Stanford University
Release Date : 2011
A Self Assembly Approach To Localization And Patterning Of Optically Resolved Single Molecules written by Randall Mark Stoltenberg and has been published by Stanford University this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
Directed assembly of single molecules is a central theme in nanotechnology. This body of work was inspired by a specific challenge involving ordered deposition of single DNAs on surfaces for massively parallel single molecule DNA sequencing via fluorescence microscopy. A potential 10-fold gain in data density is possible if single molecules can be forced into a regular array rather than randomly deposited. The dimensions of such an array are difficult to achieve with conventional lithography techniques. On one end, molecules must be separated by sufficient distance so their optical signatures do not overlap. This distance is on the order of hundreds of nanometers. On the other end, the attachment points for the molecules must have molecular dimensions. Bridging these two length scales is a formidable task. The ability to place nanometer scale objects with nanometer precision can been achieved through atomic force microscopy, scanning tunneling microscopy, optical tweezers, and ebeam lithography. All of these techniques, however, are serial in nature and hence do not serve the intended gain in data density. Another approach toward directed patterning of single molecules is through self-assembly. In this work, self-assembly of block copolymers is explored as a means to addressing the molecular and optical resolution length scales simultaneously. First, the challenge of molecular patterning for single molecule fluorescence microscopy is explored theoretically and the limits of this approach are defined. Block copolymers are introduced as a possible solution to generating the correct surface patterns for improved data density, and experimental results are compared to theoretical predictions. Second, the surface chemistry of these arrays is characterized, and I will show they can be selectively functionalized in preparation for directed assembly of DNAs. Third, these arrays are integrated into single molecule fluorescence imaging experiments to determine their potential for improved data density. What emerges from this work is not only a viable platform for increased single molecule fluorescence data density, but also a deeper understanding of the requirements for directed self-assembly of single molecules.