Restriction Enzymes
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Restriction Endonucleases
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Author : Alfred Pingoud
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Restriction Endonucleases written by Alfred Pingoud and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.
Restriction Endonucleases And Methylases
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Author : Jack G. Chirikjian
language : en
Publisher: Elsevier Publishing Company
Release Date : 1987
Restriction Endonucleases And Methylases written by Jack G. Chirikjian and has been published by Elsevier Publishing Company this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Science categories.
Restriction Endonucleases and Methylases, the fifth volume in the series, Gene Amplification and Analysis, covers important new research advances in molecular biology brought about by the use of restriction endonucleases and methylases. Contributions by authorities in the field focus on research studies that examine both the structure and catalytic properties of this important group of DNA enzymes. This volume also serves as a useful reference for reaction conditions and the general application of restriction endonucleases as reagents for molecular biology. Restriction Endonucleases and Methylases is invaluable reading for those who wish to better understand the restriction enzymes utilized in various areas of research in molecular biology.
Restriction Enzymes
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Author : Wil A. M. Loenen
language : en
Publisher:
Release Date : 2019
Restriction Enzymes written by Wil A. M. Loenen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with Gene amplification categories.
Restriction enzymes cleave DNA at specific recognition sites and have many uses in molecular biology, genetics, and biotechnology. More than 4000 restriction enzymes are known today, of which more than 621 are commercially available, justifying their description by Nobel Prize winner Richard Roberts as "the workhorses of molecular biology." This book by Wil Loenen is the first full-length history of these invaluable tools, from their recognition in the 1950s to the flowering of their development in the 1970s and 1980s to their ubiquitous availability today. Loenen has worked with restriction enzymes throughout her career as a research scientist, during which she came to know many of the leaders in this field personally and professionally. She is the author of several authoritative and widely appreciated reviews of the enzymes' biology. Her book was written with the close assistance of several of the field's pioneers, including Rich Roberts, Stuart Linn, Tom Bickle, Steve Halford, and the late Joe Bertani. The seed for the book was sown at a retirement party for Noreen Murray, to whom the book is dedicated, and its roots lie in a remarkable 2013 conference at Cold Spring Harbor Laboratory that celebrated the people and events that were vital to the field's development. Funding for the book was made possible by the Genentech Center for the History of Molecular Biology and Biotechnology at Cold Spring Harbor Laboratory.
Restriction Enzymes
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Author : Source Wikipedia
language : en
Publisher: University-Press.org
Release Date : 2013-09
Restriction Enzymes written by Source Wikipedia and has been published by University-Press.org this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-09 with categories.
Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Pages: 57. Chapters: BamHI, BglII, BpuJI, Cfr10I/Bse634I, EcoRI, EcoRV, FokI, HaeIII, HindII, HindIII, Homing endonuclease, I-CreI, Isocaudomer, Isoschizomer, List of homing endonuclease cutting sites, List of restriction enzyme cutting sites, List of restriction enzyme cutting sites: A, List of restriction enzyme cutting sites: Ba-Bc, List of restriction enzyme cutting sites: Bd-Bp, List of restriction enzyme cutting sites: Bsa-Bso, List of restriction enzyme cutting sites: Bsp-Bss, List of restriction enzyme cutting sites: Bst-Bv, List of restriction enzyme cutting sites: C-D, List of restriction enzyme cutting sites: E-F, List of restriction enzyme cutting sites: G-K, List of restriction enzyme cutting sites: L-N, List of restriction enzyme cutting sites: O-R, List of restriction enzyme cutting sites: S, List of restriction enzyme cutting sites: T-Z, Meganuclease I-SceI, NdeI, Neoschizomer, NlaIII, PstI, R.EcoRII, Rare-cutter enzyme, Rebase (database), Restriction fragment, Star activity, TaqI, XhoI. Excerpt: This article contains a list of the most studied restriction enzymes whose names start with A. An approximated number of 130 enzymes has been included in the next table. The following information is given: The cutting of DNA at specific boundaries A restriction enzyme (or restriction endonuclease) is an enzyme that cuts DNA at specific recognition nucleotide sequences (with Type II restriction enzymes cutting double-stranded DNA) known as restriction sites. Such enzymes, found in bacteria and archaea, are thought to have evolved to provide a defense mechanism against invading viruses. Inside a bacterial host, the restriction enzymes selectively cut up foreign DNA in a process called restriction; host DNA is methylated by a modification enzyme (a methylase) to protect it from the restriction enzyme's activity. Collectively, ...
Restriction Endonucleases
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Author : Jack G. Chirikjian
language : en
Publisher: North Holland
Release Date : 1981
Restriction Endonucleases written by Jack G. Chirikjian and has been published by North Holland this book supported file pdf, txt, epub, kindle and other format this book has been release on 1981 with Science categories.
Enzymes In Molecular Biology
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Author : C. J. McDonald
language : en
Publisher: Wiley-Interscience
Release Date : 1996
Enzymes In Molecular Biology written by C. J. McDonald and has been published by Wiley-Interscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Medical categories.
A useful source for the most commonly used enzymes for the manipulation of DNA, RNA and proteins. It is carefully organized to list the essential data for each class of enzyme in a uniform yet readable way. Each enzyme entry lists its properties, applications, problems, and much more.
Production And Characterization Of Recombinant Restriction Enzyme Ngomiv And Its Mutant
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Author : Wei Ren
language : en
Publisher:
Release Date : 2012
Production And Characterization Of Recombinant Restriction Enzyme Ngomiv And Its Mutant written by Wei Ren and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
Discovery of restriction enzymes has led to the development of recombinant DNA technology and restriction enzymes are currently widely used in biotechnology, medicine and research. Till now, thousands of restriction enzymes have been discovered, purified, characterized and commercialized. As well as, production of restriction enzymes has gained a huge profit in biotechnology industry. Although restriction enzymes have been applied well in current molecular biology researches, a lot of questions regarding their binding and catalytic mechanisms haven't been clearly explained or described by scientists. NgoMIV is a tetrameric restriction enzyme, which has been discovered and purified about 20 years ago from Neisseria gonorrhoeae MS11. It belongs to the type II P and type II F subtypes. The recognition site of NgoMIV is 5'-G/CCGGC-3', which is a palindromic sequence. Also the tetrameric protein has two catalytic cores, which means that it will bind with two cognate sites and cut them both. NgoAIV, which was discovered in another strain- FA1090 of Neisseria gonorrhoeae, is different from NgoMIV protein in two amino-acid residues. A hybrid protein called NgoIV-hb was designed based on the differences. In the thesis study here, the expression of NgoMIV and its mutant was conducted. To undercover the preference of the distance between two recognition sites, digestion tests by applying a plasmid with four recognition sites as substrate were conducted. At last, several proposed mechanisms of catalysis based on the protein motion predicted by anisotropic elastic network model and current existing crystal structures were raised.
Restriction Enzyme Digestion Patterns Of Acanthamoeba Castellanii Using Twenty Seven Restriction Enzymes
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Author : Rommel S. Que
language : en
Publisher:
Release Date : 1996
Restriction Enzyme Digestion Patterns Of Acanthamoeba Castellanii Using Twenty Seven Restriction Enzymes written by Rommel S. Que and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with categories.
Restriction Enzyme Database Rebase
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Author :
language : en
Publisher:
Release Date :
Restriction Enzyme Database Rebase written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Features the Restriction Enzyme Database (REBASE), a collection of information about restriction enzymes, methylases, recognition sequences, cleavage sites, methylation specificity, and more. Notes that the database is compiled by Richard J. Roberts and Dana Macelis and is updated daily.
Recombinant Dna Technology
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Author : Siddra Ijaz
language : en
Publisher: Cambridge Scholars Publishing
Release Date : 2019-08-13
Recombinant Dna Technology written by Siddra Ijaz and has been published by Cambridge Scholars Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-13 with Science categories.
Genetic engineering is a rapidly growing field in the area of biological sciences. The driving forces behind this are the challenges encountered by health sectors, agriculture, the environment, and industry. As such, accurate and comprehensive knowledge about the philosophy, principles and application of genetic engineering is indispensable for students and researchers to harness maximum opportunities from this field of science. This volume gathers together comprehensive information regarding genetic engineering from recent studies, and presents it in a coherent manner. As such, it will be of interest to undergraduate and postgraduate students and researchers working in the biological sciences.