Restriction Endonucleases
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Restriction Endonucleases
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Author : Alfred Pingoud
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Restriction Endonucleases written by Alfred Pingoud and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.
Restriction Endonucleases And Methylases
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Author : Jack G. Chirikjian
language : en
Publisher: Elsevier Publishing Company
Release Date : 1987
Restriction Endonucleases And Methylases written by Jack G. Chirikjian and has been published by Elsevier Publishing Company this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Science categories.
Restriction Endonucleases and Methylases, the fifth volume in the series, Gene Amplification and Analysis, covers important new research advances in molecular biology brought about by the use of restriction endonucleases and methylases. Contributions by authorities in the field focus on research studies that examine both the structure and catalytic properties of this important group of DNA enzymes. This volume also serves as a useful reference for reaction conditions and the general application of restriction endonucleases as reagents for molecular biology. Restriction Endonucleases and Methylases is invaluable reading for those who wish to better understand the restriction enzymes utilized in various areas of research in molecular biology.
Restriction Endonucleases
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Author : Jack G. Chirikjian
language : en
Publisher: North Holland
Release Date : 1981
Restriction Endonucleases written by Jack G. Chirikjian and has been published by North Holland this book supported file pdf, txt, epub, kindle and other format this book has been release on 1981 with Science categories.
Structure And Function Of The Restriction Endonucleases Bse634i And Muni
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Author :
language : en
Publisher:
Release Date : 2006
Structure And Function Of The Restriction Endonucleases Bse634i And Muni written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.
The type II restriction endonucleases specifically recognise symmetric DNA sequence of 4 to 8 base pairs and cut the DNA strand within or close to their recognition site. Fidelity of these enzymes is remarkable: a change of just one base pair in a specific recognition site can reduce cleavage rates 105 to 109 times. This property makes type II restriction endonucleases a useful tool for molecular biologists and an interesting model for studying DNA-protein interactions. The basis of restriction endonuclease fidelity could not been understood from analysis of their sequences alone, because these sequences share in general little similarity. It was necessary to solve three dimensional structures of typical type II restriction enzymes to find out how they interact with DNA and to suggest hypotheses explaining recognition and catalysis mechanisms of these enzymes. Surprisingly, the structures show that restriction enzymes can employ different strategies of DNA recognition even for their common inner base pairs. Thus, a minimal difference of single base pair in recognition site may select enzymes with relatively large differences in their DNA recognition machinery. We have therefore decided to investigate the variability of restriction enzymes with the same recognition sequence and chose a pair of isoshisomeric restriction endonucleases, Bse634I and Cfr10I, for our structural studies. Both enzymes recognise degenerate DNA sequence 5'-Pu/CCGGPy, and cut as indicated by /. Structure of Cfr10I is already known; in this work we report the structure of Bse634I restriction enzyme from Bacillus stearothermophilus. The two enzymes, Bse634I and Cfr10I, are the first pair of isoshisomeric restriction endonucleases where structures of both proteins in a DNA-free state are known. Comparison of the two structures shows how DNA recognition and catalysis are possibly coupled through the rearrangement of the mobile protein subdomains.
Restriction Enzymes
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Author : Wil A. M. Loenen
language : en
Publisher:
Release Date : 2019
Restriction Enzymes written by Wil A. M. Loenen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with Gene amplification categories.
Restriction enzymes cleave DNA at specific recognition sites and have many uses in molecular biology, genetics, and biotechnology. More than 4000 restriction enzymes are known today, of which more than 621 are commercially available, justifying their description by Nobel Prize winner Richard Roberts as "the workhorses of molecular biology." This book by Wil Loenen is the first full-length history of these invaluable tools, from their recognition in the 1950s to the flowering of their development in the 1970s and 1980s to their ubiquitous availability today. Loenen has worked with restriction enzymes throughout her career as a research scientist, during which she came to know many of the leaders in this field personally and professionally. She is the author of several authoritative and widely appreciated reviews of the enzymes' biology. Her book was written with the close assistance of several of the field's pioneers, including Rich Roberts, Stuart Linn, Tom Bickle, Steve Halford, and the late Joe Bertani. The seed for the book was sown at a retirement party for Noreen Murray, to whom the book is dedicated, and its roots lie in a remarkable 2013 conference at Cold Spring Harbor Laboratory that celebrated the people and events that were vital to the field's development. Funding for the book was made possible by the Genentech Center for the History of Molecular Biology and Biotechnology at Cold Spring Harbor Laboratory.
Restriction Endonucleases From Haemophilus Species
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Author : June Hill Middleton
language : en
Publisher:
Release Date : 1973
Restriction Endonucleases From Haemophilus Species written by June Hill Middleton and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1973 with Endonucleases categories.
Molecular Biology Of Nucleases
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Author : Nawin C. Mishra
language : en
Publisher: CRC Press
Release Date : 1995-03-06
Molecular Biology Of Nucleases written by Nawin C. Mishra and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-03-06 with Medical categories.
Nucleases occupy a central position in the biochemistry of DNA transactions and other metabolism of nucleic acids in all organisms. They have also proven useful in modern biological studies crucial for the development of recombinant DNA technology and reverse genetics. Nucleases assist in the identification and characterization of genes responsible for several diseases and their possible alleviation by gene therapy. Molecular Biology of Nucleases introduces the properties and biological roles of nucleases. It is the one comprehensive source for newcomers to the field.
Nucleases
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Author : Stuart M. Linn
language : en
Publisher:
Release Date : 1982
Nucleases written by Stuart M. Linn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1982 with Medical categories.
A Study Of Restriction Endonucleases Electrophoresing Techniques And Procedures For Mapping Dna
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Author : Donald F. Dones
language : en
Publisher:
Release Date : 1985
A Study Of Restriction Endonucleases Electrophoresing Techniques And Procedures For Mapping Dna written by Donald F. Dones and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985 with categories.
Homing Endonucleases
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Author : David Edgell
language : en
Publisher: Humana Press
Release Date : 2014-02-09
Homing Endonucleases written by David Edgell and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-02-09 with Medical categories.
Homing Endonucleases: Methods and Protocols aims at providing molecular biologists with a comprehensive resource to identify and characterize homing endonucleases from genomic sequence, to deduce the biological basis of binding and cleavage specificity, as well as to provide protocols to redesign endonuclease target specificity for genome-editing applications. Engineering of designer homing endonucleases has set the stage for genome editing of complex eukaryotic genomes with a broad range of potential applications including targeted gene knockouts in model organisms and gene therapy in humans, making this book a valuable resource for future research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Homing Endonucleases: Methods and Protocols serves as a key reference for all labs studying site-specific DNA endonucleases.