Molecular Biology Of Nucleases
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Molecular Biology Of Nucleases
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Author : Nawin C. Mishra
language : en
Publisher: CRC Press
Release Date : 1995-03-06
Molecular Biology Of Nucleases written by Nawin C. Mishra and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-03-06 with Medical categories.
Nucleases occupy a central position in the biochemistry of DNA transactions and other metabolism of nucleic acids in all organisms. They have also proven useful in modern biological studies crucial for the development of recombinant DNA technology and reverse genetics. Nucleases assist in the identification and characterization of genes responsible for several diseases and their possible alleviation by gene therapy. Molecular Biology of Nucleases introduces the properties and biological roles of nucleases. It is the one comprehensive source for newcomers to the field.
Nucleases
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Author : Mishra
language : en
Publisher:
Release Date : 2003-05-01
Nucleases written by Mishra and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-05-01 with categories.
Nucleases
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Author : Nawin C. Mishra
language : en
Publisher: Wiley-Interscience
Release Date : 2002-09-06
Nucleases written by Nawin C. Mishra and has been published by Wiley-Interscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-09-06 with Medical categories.
* Provides detailed applications of nucleases in recombinant DNA technology, molecular cloning, biotechnology, pharmaceuticals, and commerce. * Covers the role of nucleases in biological systems, with focus on understanding their role in causing human diseases.
Artificial Nucleases
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Author : Marina A. Zenkova
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Artificial Nucleases written by Marina A. Zenkova and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The development of agents capable of cleaving RNA and DNA has attracted considerable attention from researchers in the last few years, because of the immediate and very important applications they can find in the emerging fields of biotechnology and pharmacology. There are essentially two classes of these agents - nucleases that occur naturally inside cells and synthetically produced artificial nucleases. The first class includes protein enzyme nucle ases and catalytic RNA structured ribozymes that perform cleavage of the phosphodiester bonds in nucleic acids according to a hydrolytic pathway in the course of different biochemical processes in the cell. A different pathway is used by some antibiotics which cleave DNA via redox-based mechanisms resulting in oxidative damage of nucleotide units and breakage of the DNA backbone. The above molecules are indispensable tools for manipulating nucleic acids and processing RNA; DNA-cleaving antibiotics and cytotoxic ribonucleases have demonstrated utility as chemotherapeutic agents. The second class, artificial nucleases, are rationally designed to imitate the active centers of natural enzymes by simple structures possessing minimal sets of the most important characteristics that are essential for catalysis. A dif ferent approach, in vitro selection, was also used to create artificial RNA and DNA enzymes capable of cleaving RNA. Being less efficient and specific as compared to the natural enzymes, the primitive mimics are smaller and robust and can function in a broad range of conditions.
Nuclease Methods And Protocols
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Author : Catherine H. Schein
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Nuclease Methods And Protocols written by Catherine H. Schein and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Nucleases, enzymes that restructure or degrade nucleic acid polymers, are vital to the control of every area of metabolism. They range from “housekeeping” enzymes with broad substrate ranges to extremely specific tools (1). Many types of nucleases are used in lab protocols, and their commercial and clinical uses are expanding. The purpose of Nuclease Methods and Protocols is to introduce the reader to some we- characterized protein nucleases, and the methods used to determine their activity, structure, interaction with other molecules, and physiological role. Each chapter begins with a mini-review on a specific nuclease or a nuclease-related theme. Although many chapters cover several topics, they were arbitrarily divided into five parts: Part I, “Characterizing Nuclease Activity,” includes protocols and assays to determine general (processive, distributive) or specific mechanisms. Methods to assay nuclease products, identify cloned nucleases, and determine their physiological role are also included here. Part II, “Inhibitors and Activators of Nucleases,” summarizes assays for measuring the effects of other proteins and small molecules. Many of these inhibitors have clinical relevance. Part III, “Relating Nuclease Structure and Function,” provides an overview of methods to determine or model the 3-D structure of nucleases and their complexes with substrates and inhibitors. A 3-D structure can greatly aid the rational design of nucleases and inhibitors for specific purposes. Part IV, “Nucleases in the Clinic,” summarizes assays and protocols suitable for use with t- sues and for nuclease based therapeutics.
Metabolism And Enzymology Of Nucleic Acids
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Author : Jan Zelinka
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Metabolism And Enzymology Of Nucleic Acids written by Jan Zelinka and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
Papers of the June 1987 meeting. Sponsored by the Slovak Academy of Sciences, most contributors are from Eastern European countries. Beyond the obvious nucleic acids and nucleases, topics include methylation, molecular cloning, gene expression. Annotation copyright Book News, Inc. Portland, Or.
Nuclease Methods And Protocols
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Author : Catherine H. Schein
language : en
Publisher: Humana Press
Release Date : 2001-01-30
Nuclease Methods And Protocols written by Catherine H. Schein and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-01-30 with Science categories.
Nucleases, enzymes that restructure or degrade nucleic acid polymers, are vital to the control of every area of metabolism. They range from “housekeeping” enzymes with broad substrate ranges to extremely specific tools (1). Many types of nucleases are used in lab protocols, and their commercial and clinical uses are expanding. The purpose of Nuclease Methods and Protocols is to introduce the reader to some we- characterized protein nucleases, and the methods used to determine their activity, structure, interaction with other molecules, and physiological role. Each chapter begins with a mini-review on a specific nuclease or a nuclease-related theme. Although many chapters cover several topics, they were arbitrarily divided into five parts: Part I, “Characterizing Nuclease Activity,” includes protocols and assays to determine general (processive, distributive) or specific mechanisms. Methods to assay nuclease products, identify cloned nucleases, and determine their physiological role are also included here. Part II, “Inhibitors and Activators of Nucleases,” summarizes assays for measuring the effects of other proteins and small molecules. Many of these inhibitors have clinical relevance. Part III, “Relating Nuclease Structure and Function,” provides an overview of methods to determine or model the 3-D structure of nucleases and their complexes with substrates and inhibitors. A 3-D structure can greatly aid the rational design of nucleases and inhibitors for specific purposes. Part IV, “Nucleases in the Clinic,” summarizes assays and protocols suitable for use with t- sues and for nuclease based therapeutics.
Restriction Endonucleases
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Author : Alfred Pingoud
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Restriction Endonucleases written by Alfred Pingoud and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.
Progress In Nucleic Acid Research And Molecular Biology
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Author :
language : en
Publisher: Academic Press
Release Date : 1981-07-23
Progress In Nucleic Acid Research And Molecular Biology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1981-07-23 with Medical categories.
Progress in Nucleic Acid Research and Molecular Biology
Nucleases
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Author : Stuart M. Linn
language : en
Publisher:
Release Date : 1985
Nucleases written by Stuart M. Linn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985 with Deoxyribonuclease categories.