Nucleases

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Nuclease Methods And Protocols

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Author : Catherine H. Schein
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03

Nuclease Methods And Protocols written by Catherine H. Schein and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.

Nucleases, enzymes that restructure or degrade nucleic acid polymers, are vital to the control of every area of metabolism. They range from “housekeeping” enzymes with broad substrate ranges to extremely specific tools (1). Many types of nucleases are used in lab protocols, and their commercial and clinical uses are expanding. The purpose of Nuclease Methods and Protocols is to introduce the reader to some we- characterized protein nucleases, and the methods used to determine their activity, structure, interaction with other molecules, and physiological role. Each chapter begins with a mini-review on a specific nuclease or a nuclease-related theme. Although many chapters cover several topics, they were arbitrarily divided into five parts: Part I, “Characterizing Nuclease Activity,” includes protocols and assays to determine general (processive, distributive) or specific mechanisms. Methods to assay nuclease products, identify cloned nucleases, and determine their physiological role are also included here. Part II, “Inhibitors and Activators of Nucleases,” summarizes assays for measuring the effects of other proteins and small molecules. Many of these inhibitors have clinical relevance. Part III, “Relating Nuclease Structure and Function,” provides an overview of methods to determine or model the 3-D structure of nucleases and their complexes with substrates and inhibitors. A 3-D structure can greatly aid the rational design of nucleases and inhibitors for specific purposes. Part IV, “Nucleases in the Clinic,” summarizes assays and protocols suitable for use with t- sues and for nuclease based therapeutics.

Molecular Biology Of Nucleases

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Author : Nawin C. Mishra
language : en
Publisher: CRC Press
Release Date : 1995-03-06

Molecular Biology Of Nucleases written by Nawin C. Mishra and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-03-06 with Medical categories.

Nucleases occupy a central position in the biochemistry of DNA transactions and other metabolism of nucleic acids in all organisms. They have also proven useful in modern biological studies crucial for the development of recombinant DNA technology and reverse genetics. Nucleases assist in the identification and characterization of genes responsible for several diseases and their possible alleviation by gene therapy. Molecular Biology of Nucleases introduces the properties and biological roles of nucleases. It is the one comprehensive source for newcomers to the field.

Nucleases

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Author : Nawin C. Mishra
language : en
Publisher: Wiley-Interscience
Release Date : 2002-09-06

Nucleases written by Nawin C. Mishra and has been published by Wiley-Interscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-09-06 with Medical categories.

* Provides detailed applications of nucleases in recombinant DNA technology, molecular cloning, biotechnology, pharmaceuticals, and commerce. * Covers the role of nucleases in biological systems, with focus on understanding their role in causing human diseases.

Artificial Nucleases

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Author : Marina A. Zenkova
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Artificial Nucleases written by Marina A. Zenkova and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

The development of agents capable of cleaving RNA and DNA has attracted considerable attention from researchers in the last few years, because of the immediate and very important applications they can find in the emerging fields of biotechnology and pharmacology. There are essentially two classes of these agents - nucleases that occur naturally inside cells and synthetically produced artificial nucleases. The first class includes protein enzyme nucle ases and catalytic RNA structured ribozymes that perform cleavage of the phosphodiester bonds in nucleic acids according to a hydrolytic pathway in the course of different biochemical processes in the cell. A different pathway is used by some antibiotics which cleave DNA via redox-based mechanisms resulting in oxidative damage of nucleotide units and breakage of the DNA backbone. The above molecules are indispensable tools for manipulating nucleic acids and processing RNA; DNA-cleaving antibiotics and cytotoxic ribonucleases have demonstrated utility as chemotherapeutic agents. The second class, artificial nucleases, are rationally designed to imitate the active centers of natural enzymes by simple structures possessing minimal sets of the most important characteristics that are essential for catalysis. A dif ferent approach, in vitro selection, was also used to create artificial RNA and DNA enzymes capable of cleaving RNA. Being less efficient and specific as compared to the natural enzymes, the primitive mimics are smaller and robust and can function in a broad range of conditions.

Nucleases

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Author : Mishra
language : en
Publisher:
Release Date : 2003-05-01

Nucleases written by Mishra and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-05-01 with categories.

Nucleases

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Author : Stuart M. Linn
language : en
Publisher:
Release Date : 1985

Nucleases written by Stuart M. Linn and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985 with Deoxyribonuclease categories.

Targeted Genome Editing Using Site Specific Nucleases

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Author : Takashi Yamamoto
language : en
Publisher: Springer
Release Date : 2015-01-05

Targeted Genome Editing Using Site Specific Nucleases written by Takashi Yamamoto and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-01-05 with Science categories.

This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.

Rna Turnover In Eukaryotes Nucleases Pathways And Analysis Of Mrna Decay

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Author : Lynne E. Maquat
language : en
Publisher: Academic Press
Release Date : 2009-01-30

Rna Turnover In Eukaryotes Nucleases Pathways And Analysis Of Mrna Decay written by Lynne E. Maquat and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-30 with Science categories.

Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation. Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway Expert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover, which are central to the process of gene expression Offers step-by-step lab instructions, including necessary equipment and reagents

Nicht Konventionelle Verfahren Zur Herstellung Textiler Flaechengebilde

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Author :
language : en
Publisher:
Release Date : 1976

Nicht Konventionelle Verfahren Zur Herstellung Textiler Flaechengebilde written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1976 with categories.

Metabolism And Enzymology Of Nucleic Acids

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Author : Jan Zelinka
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Metabolism And Enzymology Of Nucleic Acids written by Jan Zelinka and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.

Papers of the June 1987 meeting. Sponsored by the Slovak Academy of Sciences, most contributors are from Eastern European countries. Beyond the obvious nucleic acids and nucleases, topics include methylation, molecular cloning, gene expression. Annotation copyright Book News, Inc. Portland, Or.