Structure And Function Of The Restriction Endonucleases Bse634i And Muni
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Structure And Function Of The Restriction Endonucleases Bse634i And Muni
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Author : Saulius Gražulis
language : en
Publisher:
Release Date : 2000
Structure And Function Of The Restriction Endonucleases Bse634i And Muni written by Saulius Gražulis and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with categories.
Structure And Function Of The Restriction Endonucleases Bse634i And Muni
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Author :
language : en
Publisher:
Release Date : 2006
Structure And Function Of The Restriction Endonucleases Bse634i And Muni written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with categories.
The type II restriction endonucleases specifically recognise symmetric DNA sequence of 4 to 8 base pairs and cut the DNA strand within or close to their recognition site. Fidelity of these enzymes is remarkable: a change of just one base pair in a specific recognition site can reduce cleavage rates 105 to 109 times. This property makes type II restriction endonucleases a useful tool for molecular biologists and an interesting model for studying DNA-protein interactions. The basis of restriction endonuclease fidelity could not been understood from analysis of their sequences alone, because these sequences share in general little similarity. It was necessary to solve three dimensional structures of typical type II restriction enzymes to find out how they interact with DNA and to suggest hypotheses explaining recognition and catalysis mechanisms of these enzymes. Surprisingly, the structures show that restriction enzymes can employ different strategies of DNA recognition even for their common inner base pairs. Thus, a minimal difference of single base pair in recognition site may select enzymes with relatively large differences in their DNA recognition machinery. We have therefore decided to investigate the variability of restriction enzymes with the same recognition sequence and chose a pair of isoshisomeric restriction endonucleases, Bse634I and Cfr10I, for our structural studies. Both enzymes recognise degenerate DNA sequence 5'-Pu/CCGGPy, and cut as indicated by /. Structure of Cfr10I is already known; in this work we report the structure of Bse634I restriction enzyme from Bacillus stearothermophilus. The two enzymes, Bse634I and Cfr10I, are the first pair of isoshisomeric restriction endonucleases where structures of both proteins in a DNA-free state are known. Comparison of the two structures shows how DNA recognition and catalysis are possibly coupled through the rearrangement of the mobile protein subdomains.
Restriction Endonucleases
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Author : Alfred Pingoud
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Restriction Endonucleases written by Alfred Pingoud and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.
Restriction Enzymes
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Author : Wil A. M. Loenen
language : en
Publisher:
Release Date : 2019
Restriction Enzymes written by Wil A. M. Loenen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with Gene amplification categories.
Restriction enzymes cleave DNA at specific recognition sites and have many uses in molecular biology, genetics, and biotechnology. More than 4000 restriction enzymes are known today, of which more than 621 are commercially available, justifying their description by Nobel Prize winner Richard Roberts as "the workhorses of molecular biology." This book by Wil Loenen is the first full-length history of these invaluable tools, from their recognition in the 1950s to the flowering of their development in the 1970s and 1980s to their ubiquitous availability today. Loenen has worked with restriction enzymes throughout her career as a research scientist, during which she came to know many of the leaders in this field personally and professionally. She is the author of several authoritative and widely appreciated reviews of the enzymes' biology. Her book was written with the close assistance of several of the field's pioneers, including Rich Roberts, Stuart Linn, Tom Bickle, Steve Halford, and the late Joe Bertani. The seed for the book was sown at a retirement party for Noreen Murray, to whom the book is dedicated, and its roots lie in a remarkable 2013 conference at Cold Spring Harbor Laboratory that celebrated the people and events that were vital to the field's development. Funding for the book was made possible by the Genentech Center for the History of Molecular Biology and Biotechnology at Cold Spring Harbor Laboratory.
Hawaii Volcanoes National Park Hawaii
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Author : United States. National Park Service
language : en
Publisher:
Release Date : 1968
Hawaii Volcanoes National Park Hawaii written by United States. National Park Service and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1968 with Hawaii Volcanoes National Park (Hawaii) categories.
Biotransformations
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Author : K. Faber
language : en
Publisher: Springer Science & Business Media
Release Date : 2003-06-30
Biotransformations written by K. Faber and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-06-30 with Science categories.
Whereas the hydrolases such as proteases, esterases and lipases are sufficiently well researched to be applied in every standard laboratory, other types of enzymes are still waiting to be discovered with respect to their applicability in organic-chemistry transformations on a preparative scale. This latter point is stressed here, with the focus on the newcomer-enzymes'which show great synthetic potential.
Biocatalysis
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Author : Andreas S. Bommarius
language : de
Publisher: John Wiley & Sons
Release Date : 2007-02-27
Biocatalysis written by Andreas S. Bommarius and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-02-27 with Science categories.
The whole range of biocatalysis, from a firm grounding in theoretical concepts to in-depth coverage of practical applications and future perspectives. The book not only covers reactions, products and processes with and from biological catalysts, but also the process of designing and improving such biocatalysts. One unique feature is that the fields of chemistry, biology and bioengineering receive equal attention, thus addressing practitioners and students from all three areas.
Biocatalysis In Organic Synthesis
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Author : Nicholas J. Turner
language : en
Publisher: Royal Society of Chemistry
Release Date : 2018-02-08
Biocatalysis In Organic Synthesis written by Nicholas J. Turner and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-02-08 with Science categories.
The application of biocatalysis in organic synthesis is rapidly gaining popularity amongst chemists. Compared to traditional synthetic methodologies biocatalysis offers a number of advantages in terms of enhanced selectivity (chemo-, regio-, stereo-), reduced environmental impact and lower cost of starting materials. Together these advantages can contribute to more sustainable manufacturing processes across a wide range of industries ranging from pharmaceuticals to biofuels. The biocatalytic toolbox has expanded significantly in the past five years and given the current rate of development of new engineered biocatalysts it is likely that the number of available biocatalysts will double in the next few years. This textbook gives a comprehensive overview of the current biocatalytic toolbox and also establishes new guidelines or rules for “biocatalytic retrosynthesis”. Retrosynthesis is a well known and commonly used technique whereby organic chemists start with the structure of their target molecule and generate potential starting materials and intermediates through a series of retrosynthetic disconnections. These disconnections are then used to devise a forward synthesis, in this case using biocatalytic transformations in some of the key steps. Target molecules are disconnected with consideration for applying biocatalysts, as well as chemical reagents and chemocatalysts, in the forward synthesis direction. Using this textbook, students will be able to place biocatalysis within the context of other synthetic transformations that they have learned earlier in their studies. This additional awareness of biocatalysis will equip students for the modern world of organic synthesis where biocatalysts play an increasingly important role. In addition to guidelines for identifying where biocatalysts can be applied in organic synthesis, this textbook also provides examples of current applications of biocatalysis using worked examples and case studies. Tutorials enable the reader to practice disconnecting target molecules to find the ‘hidden’ biocatalytic reactions which can be applied in the synthetic direction. The book contains a complete description of the current biocatalyst classes that are available for use and also suggests areas where new enzymes are likely to be developed in the next few years. This textbook is an essential resource for lecturers and students studying synthetic organic chemistry. It also serves as a handy reference for practicing chemists who wish to embed biocatalysis into their synthetic toolbox.
Dna And Rna Modification Enzymes
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Author : Henri Grosjean
language : en
Publisher: CRC Press
Release Date : 2009-06-11
Dna And Rna Modification Enzymes written by Henri Grosjean and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-06-11 with Science categories.
This volume is a timely and comprehensive description of the many facets of DNA and RNA modification-editing processes and to some extent repair mechanisms. Each chapter offers fundamental principles as well as up to date information on recent advances in the field (up to end 2008). They ended by a short ‘conclusion and future prospect’ section and an exhaustive list of 35 to up to 257 references (in average 87). Contributors are geneticists, structural enzymologists and molecular biologists working at the forefront of this exciting, fast-moving and diverse field of researches. This book will be a major interest to PhD students and University teachers alike. It will also serve as an invaluable reference tool for new researchers in the field, as well as for specialists of RNA modification enzymes generally not well informed about what is going on in similar processes acting on DNA and vice-versa for specialists of the DNA modification-editing and repair processes usually not much acquainted with what is going on in the RNA maturation field. The book is subdivided into 41 chapters (740 pages). The common links between them are mainly the enzymatic aspects of the different modification-editing and repair machineries: structural, mechanistic, functional and evolutionary aspects. It starts with two general and historical overview of the discovery of modified nucleosides in DNA and RNA and corresponding modification-editing enzymes. Then follows eleven chapters on DNA modification and editing (mechanistic and functional aspects). Two additional chapters cover problems related to DNA/RNA repair and base editing by C-to-U deaminases, followed by three chapters on RNA editing by C-to-U and A-to-I type of deamination. Discussions about interplay between DNA and RNA modifications and the emergence of DNA are covered in two independent chapters, followed by twenty chapters on different but complementary aspects of RNA modification enzymes and their cellular implications. The last chapter concerns the description of the present state-of-the art for incorporating modified nucleosides by in vitro chemical synthesis. At the end of the book, six appendicies give useful details on modified nucleosides, modification-editing enzymes and nucleosides analogs. This information is usually difficult to obtain from current scientific literature.
The Eighth Day Of Creation
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Author : Horace Freeland Judson
language : en
Publisher:
Release Date : 2004-01-01
The Eighth Day Of Creation written by Horace Freeland Judson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-01-01 with categories.