Second Harmonic Generation Imaging

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Second Harmonic Generation Imaging

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Author : Francesco S. Pavone
language : en
Publisher: Taylor & Francis
Release Date : 2016-04-19

Second Harmonic Generation Imaging written by Francesco S. Pavone and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Science categories.

Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a

Second Harmonic Generation Imaging

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Author : Francesco Pavone
language : en
Publisher:
Release Date : 2016

Second Harmonic Generation Imaging written by Francesco Pavone and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with Fluorescence microscopy categories.

Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a.

Multispectral Imaging And Second Harmonic Generation In Scanning Laser Optical Tomography

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Author : Lena Nolte
language : en
Publisher:
Release Date : 2019

Multispectral Imaging And Second Harmonic Generation In Scanning Laser Optical Tomography written by Lena Nolte and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with Multispectral imaging categories.

Optical Imaging And Microscopy

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Author : Peter Török
language : en
Publisher: Springer
Release Date : 2007-08-17

Optical Imaging And Microscopy written by Peter Török and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-17 with Science categories.

This text on contemporary optical systems is intended for optical researchers and engineers, graduate students and optical microscopists in the biological and biomedical sciences. In three sections, the book discusses high-aperture optical systems, nonlinear optical techniques, and various techniques that are finding new applications. The new second edition has been thoroughly revised and expanded to account for new advances in fluorescence imaging and diffractive optical lenses.

Texture Analysis Applied To Second Harmonic Generation Image Data For Disease Classification And Development Of A Multi View Second Harmonic Generation Imaging Platform

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Author :
language : en
Publisher:
Release Date : 2016

Texture Analysis Applied To Second Harmonic Generation Image Data For Disease Classification And Development Of A Multi View Second Harmonic Generation Imaging Platform written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with categories.

Many diseases, e.g. ovarian cancer, breast cancer and pulmonary fibrosis, are commonly associated with drastic alterations in surrounding connective tissue, and changes in the extracellular matrix (ECM) are associated with the vast majority of cellular processes in disease progression and carcinogenesis: cell differentiation, proliferation, biosynthetic ability, polarity, and motility. We use second harmonic generation (SHG) microscopy for imaging the ECM because it is a non-invasive, non-linear laser scanning technique with high sensitivity and specificity for visualizing fibrillar collagen. In this thesis, we are interested in developing imaging techniques to understand how the ECM, especially the collagen architecture, is remodeled in diseases To quantitate remodeling, we implement a 3D texture analysis to delineate the collagen fibrillar morphology observed in SHG microscopy images of human normal and high grade malignant ovarian tissues. In the learning stage, a dictionary of "textons"--Frequently occurring texture features that are identified by measuring the image response to a filter bank of various shapes, sizes, and orientations--is created. By calculating a representative model based on the texton distribution for each tissue type using a training set of respective mages, we then perform classification between normal and high grade malignant ovarian tissues classification based on the area under receiver operating characteristic curves (true positives versus false positives). The local analysis algorithm is a more general method to probe rapidly changing fibrillar morphologies than global analyses such as FFT. It is also more versatile than other texture approaches as the filter bank can be highly tailored to specific applications (e.g., different disease states) by creating customized libraries based on common image features. Further, we describe the development of a multi-view 3D SHG imaging platform. Unlike fluorescence microscopy, SHG excites intrinsic characteristics of collagen, bypassing the need for additional primary and secondary imaging labels. However, single view image collection from endogenous SHG contrast of collagen molecules is not "a true 3D technique," because collagen fibers oriented along the plane of the lasers used to excite them are invisible to the excitation The loss of information means that researchers cannot resolve the 3D structure of the ECM using this technique. We are developing a new, multi-view approach that involves rotation of agarose embedded sample in FEP tubing, so that the excitation beam path travels to from multiple angles, to reveal new insight in understanding the 3D collagen structure and its role in normal and diseased tissue

Handbook Of Biomedical Nonlinear Optical Microscopy

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Author : Barry R. Masters
language : en
Publisher: Oxford University Press on Demand
Release Date : 2008-05-19

Handbook Of Biomedical Nonlinear Optical Microscopy written by Barry R. Masters and has been published by Oxford University Press on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-05-19 with Medical categories.

Ideal for cell biologists, life scientists, biomedical engineers, and clinicians, this handbook provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy.

Theory And Practice Of Scanning Optical Microscopy

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Author : Tony Wilson
language : en
Publisher:
Release Date : 1984

Theory And Practice Of Scanning Optical Microscopy written by Tony Wilson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Science categories.

Multiphoton Microscopy And Fluorescence Lifetime Imaging

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Author : Karsten König
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2018-01-22

Multiphoton Microscopy And Fluorescence Lifetime Imaging written by Karsten König and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-22 with Science categories.

This monograph demonstrates the latest developments in two-photon fluorescence microscopy and second-harmonic generation (SHG) microscopy, including coverage of high-resolution microscopy methods, such as STED microscopy. A special focus lies on clinical applications of these methods, e.g. in dermatology, ophtalmology, neuro sciences and cell biology.

Toxic Chemical And Biological Agents

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Author : Giovanni Sindona
language : en
Publisher: Springer Nature
Release Date : 2020-10-19

Toxic Chemical And Biological Agents written by Giovanni Sindona and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-19 with Science categories.

This book critically assesses the current state of knowledge on new and important detection technologies, e.g. mass spectrometry, tandem mass spectrometry, biosensor detection and tissue imaging, in connection with toxic chemical and biological agents. In general, the main topics discussed concern the risks and consequences of chemical and biological agents for human health in general, with special emphasis on all biochemical and metabolic pathways including the reproductive system. The exposome, genetic risks and the environment, various health hazard agents, risk assessment, environmental assessment and preparedness, and analysis of sub-lethal effects at the molecular level are also discussed. In closing, the book provides comprehensive information on the diagnosis of exposure, and on health concerns related to toxic chemical and biological agents.

Nanoscale Photonic Imaging

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Author : Tim Salditt
language : en
Publisher: Springer Nature
Release Date : 2020-06-09

Nanoscale Photonic Imaging written by Tim Salditt and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-09 with Science categories.

This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.