Sequencing
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Next Generation Sequencing
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Author : Prashanth N Suravajhala
language : en
Publisher: CRC Press
Release Date : 2025-05-30
Next Generation Sequencing written by Prashanth N Suravajhala and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-05-30 with Science categories.
This cutting-edge reference book compiles standard operating procedures, protocols, and applications of next-generation sequencing (NGS). It discusses genomic testing applications through NGS. It pays special focus on the protocols for cataloguing variants of uncertain significance. Over the years, NGS and advanced bioinformatics approaches have allowed the transition of genomic assays into translational practices. The book covers visualisation of NGS datasets, investigation of early development impairment, and metagenome protocols. It also discusses the challenges in NGS methods. Key Points: Includes case studies of application of NGS in different taxa like humans, rodents, plants, and bacteria Compiles protocols from various reputed companies like Illumina, PacBio, and ThermoFisher Discusses the translational applications of NGS methods Reviews machine learning heuristics for NGS data interpretation Discusses emerging genomic assay technologies and characterising mechanisms of disease prevalence The book is meant for researchers and industry experts in genomics, computational biology, and bioinformatics. Chapter 7 and 9 of this book is freely available as a downloadable Open Access PDF at http://www.taylorfrancis.com under a Creative Commons [Attribution-Non Commercial-No Derivatives (CC BY-NC-ND)] 4.0 license.
Next Generation Genome Sequencing
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Author : Michal Janitz
language : en
Publisher: John Wiley & Sons
Release Date : 2011-08-24
Next Generation Genome Sequencing written by Michal Janitz and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-24 with Science categories.
Written by leading experts from industry and academia, this first single comprehensive resource addresses recent developments in next generation DNA sequencing technology and their impact on genome research, drug discovery and health care. As such, it presents a detailed comparative analysis of commercially available platforms as well as insights into alternative, emerging sequencing techniques. In addition, the book not only covers the principles of DNA sequencing techniques but also social, ethical and commercial aspects, the concept of personalized medicine and a five-year perspective of DNA sequencing.
Clinical Applications For Next Generation Sequencing
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Author : Urszula Demkow
language : en
Publisher: Academic Press
Release Date : 2015-09-10
Clinical Applications For Next Generation Sequencing written by Urszula Demkow and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-09-10 with Science categories.
Clinical Applications for Next Generation Sequencing provides readers with an outstanding postgraduate resource to learn about the translational use of NGS in clinical environments. Rooted in both medical genetics and clinical medicine, the book fills the gap between state-of-the-art technology and evidence-based practice, providing an educational opportunity for users to advance patient care by transferring NGS to the needs of real-world patients. The book builds an interface between genetic laboratory staff and clinical health workers to not only improve communication, but also strengthen cooperation. Users will find valuable tactics they can use to build a systematic framework for understanding the role of NGS testing in both common and rare diseases and conditions, from prenatal care, like chromosomal abnormalities, up to advanced age problems like dementia. - Fills the gap between state-of-the-art technology and evidence-based practice - Provides an educational opportunity which advances patient care through the transfer of NGS to real-world patient assessment - Promotes a practical tool that clinicians can apply directly to patient care - Includes a systematic framework for understanding the role of NGS testing in many common and rare diseases - Presents evidence regarding the important role of NGS in current diagnostic strategies
High Throughput Next Generation Sequencing
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Author : Young Min Kwon
language : en
Publisher: Humana Press
Release Date : 2011-03-22
High Throughput Next Generation Sequencing written by Young Min Kwon and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-03-22 with Medical categories.
Due to their novel concepts and extraordinary high-throughput sequencing capacity, the “next generation sequencing” methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities. These methods are now being recognized as essential tools for a more comprehensive and deeper understanding of the mechanisms underlying many biological processes. In High-Throughput Next Generation Sequencing: Methods and Applications, experts in the field explore the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities; however, the methods described in this book will also offer general applications relevant to the study of any living organisms. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, High-Throughput Next Generation Sequencing: Methods and Applications is an excellent collection of chapters to aid all scientists who wish to apply these innovative research tools to enhance their own pursuits in microbiology and also biology in general.
Genetics For Dummies
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Author : Tara Rodden Robinson
language : en
Publisher: John Wiley & Sons
Release Date : 2005-09-02
Genetics For Dummies written by Tara Rodden Robinson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-09-02 with Science categories.
"Want to know more about genetics? This non-intimidating guide gets you up to speed on all the fundamentals. From dominant and recessive inherited traits to the DNA double-helix, you get clear expectations in easy-to-understand terms. Plus, you'll see how people are applying genetic science to fight disease, develop new products, solve crimes ... and even clone cats." -- back cover.
Phylogenetic Analysis Of Dna Sequences
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Author : Michael M. Miyamoto
language : en
Publisher: Oxford University Press
Release Date : 1991-11-14
Phylogenetic Analysis Of Dna Sequences written by Michael M. Miyamoto and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991-11-14 with Science categories.
With increasing frequency, systematic and evolutionary biologists have turned to the techniques of molecular biology to complement their traditional morphological and anatomical approaches to questions of the historical relationship and descent among groups of animals and plants. In particular, the comparative analysis of DNA sequences is becoming a common and important focus of research attention today. The objective of this volume is to survey the emerging field of molecular systematics of DNA sequences, and to appraise the strengths and limitations of the different approaches yielded by these techniques. The contributors are an internationally recognized group of investigators from different schools and disciplines who critically address a diversity of crucial questions about DNA systematics, including DNA sequence data acquisition, phylogenetic inference, congruence and consensus problems, limitations of molecular data, and the integration of molecular and morphological data sets. The work will interest all botanists and zoologists involved in systematics, taxonomy, and evolution.
Sequences Subsequences And Consequences
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Author : Solomon W. Golomb
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-12-13
Sequences Subsequences And Consequences written by Solomon W. Golomb and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-13 with Computers categories.
Interested readers will find here the thoroughly refereed post-proceedings of the International Workshop of Sequences, Subsequences and Consequences, SSC 2007, held in Los Angeles, USA, in 2007. The 16 revised invited full papers and one revised contributed paper are presented together with three keynote lectures and were carefully reviewed and selected for the book. The theory of sequences has found practical applications in many areas of coded communications and in cryptography.
Advances In The Understanding Of Biological Sciences Using Next Generation Sequencing Ngs Approaches
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Author : Gaurav Sablok
language : en
Publisher: Springer
Release Date : 2015-07-16
Advances In The Understanding Of Biological Sciences Using Next Generation Sequencing Ngs Approaches written by Gaurav Sablok and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-07-16 with Science categories.
Provides a global view of the recent advances in the biological sciences and the adaption of the pathogen to the host plants revealed using NGS. Molecular Omic’s is now a major driving force to learn the adaption genetics and a great challenge to the scientific community, which can be resolved through the application of the NGS technologies. The availability of complete genome sequences, the respective model species for dicot and monocot plant groups, presents a global opportunity to delineate the identification, function and the expression of the genes, to develop new tools for the identification of the new genes and pathway identification. Genome-wide research tools, resources and approaches such as data mining for structural similarities, gene expression profiling at the DNA and RNA level with rapid increase in available genome sequencing efforts, expressed sequence tags (ESTs), RNA-seq, gene expression profiling, induced deletion mutants and insertional mutants, and gene expression knock-down (gene silencing) studies with RNAi and microRNAs have become integral parts of plant molecular omic’s. Molecular diversity and mutational approaches present the first line of approach to unravel the genetic and molecular basis for several traits, QTL related to disease resistance, which includes host approaches to combat the pathogens and to understand the adaptation of the pathogen to the plant host. Using NGS technologies, understanding of adaptation genetics towards stress tolerance has been correlated to the epigenetics. Naturally occurring allelic variations, genome shuffling and variations induced by chemical or radiation mutagenesis are also being used in functional genomics to elucidate the pathway for the pathogen and stress tolerance and is widely illustrated in demonstrating the identification of the genes responsible for tolerance in plants, bacterial and fungal species.
Mapping And Sequencing The Human Genome
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Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 1988-01-01
Mapping And Sequencing The Human Genome written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988-01-01 with Science categories.
There is growing enthusiasm in the scientific community about the prospect of mapping and sequencing the human genome, a monumental project that will have far-reaching consequences for medicine, biology, technology, and other fields. But how will such an effort be organized and funded? How will we develop the new technologies that are needed? What new legal, social, and ethical questions will be raised? Mapping and Sequencing the Human Genome is a blueprint for this proposed project. The authors offer a highly readable explanation of the technical aspects of genetic mapping and sequencing, and they recommend specific interim and long-range research goals, organizational strategies, and funding levels. They also outline some of the legal and social questions that might arise and urge their early consideration by policymakers.
Year Round Preschool Reading
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Author : Brenda Shelton Strickland
language : en
Publisher: Teacher Created Resources
Release Date : 2006-01-20
Year Round Preschool Reading written by Brenda Shelton Strickland and has been published by Teacher Created Resources this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-01-20 with Education categories.