Signal Transduction Protocols
DOWNLOAD
Download Signal Transduction Protocols PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Signal Transduction Protocols book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Signal Transduction Protocols
DOWNLOAD
Author : Robert C. Dickson
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Signal Transduction Protocols written by Robert C. Dickson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
In 1995, Signal Transduction Protocols, edited by David A. Kendall and Stephen J. Hill, was published in the Methods in Molecular Biology series. This second edition represents an update to that previous work with an emp- sis on new methodologies that have developed in the last few years. The goal, then and now, is to provide procedures written by experts with first-hand ex- rience in a detail that goes far beyond what is generally encountered in the “methods” section of most journals and thus actually permits a particular p- cedure to be replicated. In addition, we have had as a secondary goal the id- tification of protocols for the assay of general classes of signal transduction components that, ideally, can be adapted to the assay of any member of that class. The ability to do this has resulted in large part from the use of affini- based assays, the ease with which specific proteins can be specifically tagged, and an explosion in the availability of highly specific antibodies from comm- cial sources, especially antibodies raised against signaling proteins of human origin. The number of available approaches is, fortunately for those working in signaling research, far too great to fit within the confines of this volume, so hard choices as to what to include had to be made.
Receptor Signal Transduction Protocols
DOWNLOAD
Author : Gary B. Willars
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Receptor Signal Transduction Protocols written by Gary B. Willars and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
This second edition of Receptor Signal Transduction Protocols not only has a new editor, but also a greater focus on G-protein-coupled receptors, their properties per se, and their coupling to immediate downstream binding partners—principally, although not exclusively, the heterotrimeric G-proteins. The new edition combines updates of key chapters from the first edition, as well as a large number of new contributions covering key methodologies that have emerged, or been extended to receptor/G-protein research, in the past 5–6 years. In common with many fields, the range of methods used to assess the first steps in signal transduction are continually expanding and methods that might have been considered too specialized five years ago are now sufficiently routine to be included here. Unlike many research areas, where off-the-shelf kits have made research basically foolproof, signal transduction research still requires considerable expertise, and the methods included here are provided by internationally recognized experts in their fields who have many years of experience using the methods they describe. This not only allows each chapter to impart a clear description of the method, but also to furnish invaluable troubleshooting advice for when things do not go entirely according to plan. Once again we would like to thank the Series Editor, John Walker, for the invitation to compile this second edition, and to express our gratitude to all of the authors who have enthusiastically agreed to provide the uniformly excellent contributions.
Signal Transduction Protocols
DOWNLOAD
Author : Louis M. Luttrell
language : en
Publisher: Humana Press
Release Date : 2011-08-28
Signal Transduction Protocols written by Louis M. Luttrell and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-28 with Science categories.
It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sections, Signal Transduction Protocols, Third Edition focuses on experimental approaches to better understand the complexity of signal transduction. Introductory chapters provide perspective on several of the challenges involved in signal transduction research and offer guidance on selecting the best approaches to various types of questions. The individual chapters provide detailed experimental protocols covering various topics, from the effects of ligand binding on receptor conformation and effector coupling, to moving inside the cell in order to capture the spatial and temporal characteristics of signaling events. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Signal Transduction Protocols serves as an ideal guide to scientists of all backgrounds and is a valuable resource for future progress in the field of signal transduction research.
Receptor Signal Transduction Protocols
DOWNLOAD
Author : Gary B. Willars
language : en
Publisher: Humana Press
Release Date : 2011-06-10
Receptor Signal Transduction Protocols written by Gary B. Willars and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-10 with Science categories.
As our understanding of G protein-coupled receptor (GPCR) signal transduction continues to grow, we cannot help but be struck by the emerging complexity and the ability of this receptor superfamily to continually surprise us as new facets are discovered. In this third edition of Receptor Signal Transduction Protocols, the expert contributors have taken into account the constant evolution of the GPCR field and dealt with methods that allow researchers to address many of the important rising issues. Serving as a companion to the previous editions, this volume contains thorough revisions of some core chapters, the complete rewriting of others to encompass new technological developments since the publication of the prior editions, and the commissioning of brand new, cutting-edge chapters to expand on previous coverage. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Receptor Signal Transduction Protocols, Third Edition serves the scientific community with a broad collection of the current, practical methodologies in this highly promising field.
Receptor Signal Transduction Protocols
DOWNLOAD
Author : Gary Willars
language : en
Publisher:
Release Date : 2004
Receptor Signal Transduction Protocols written by Gary Willars and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Cell receptors categories.
In this second edition of a widely appreciated work, Receptor Signal Transduction Protocols, a panel of internationally recognized investigators presents their best methods for studying G-protein-coupled receptors (GPCRs) and events immediately downstream of their activation. This new edition combines updates of key chapters from the first edition with a large number of new contributions on the many successful methodologies that have emerged more recently. The methods are focused primarily on events at the receptor level, including ligand binding, on the genetic manipulation of receptors, the generation of model cell lines in which to study them, and the interaction and activation of G-proteins. Additional methods concentrate on receptor expression and localization, receptor internalization and post-translational modification, GPCR-protein interactions, and the use of knock-out and knock-in strategies for determining the physiological roles of receptors. The laboratory protocols follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Receptor Signal Transduction Protocols, Second Edition offers both novice and experienced investigators powerful cutting-edge techniques that provide an array of approaches and specific methods that will aid in the understanding of GPCR structure and function
Plant Virology Protocols
DOWNLOAD
Author : Gary D. Foster
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Plant Virology Protocols written by Gary D. Foster and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
The aim of Plant Virology Protocols is to provide a source of infor- tion to guide the reader through the wide range of methods involved in gen- ating transgenic plants that are resistant to plant viruses. To this end, we have commissioned a wide-ranging list of chapters that will cover the methods required for: plant virus isolation; RNA extraction; cloning coat p- tein genes; introduction of the coat protein gene into the plant genome; and testing transgenic plants for resistance. The book then moves on to treatments of the mechanisms of resistance, the problems encountered with field testing, and key ethical issues surrounding transgenic technology. Although Plant Virology Protocols deals with the cloning and expression of the coat protein gene, the techniques described can be equally applied to other viral genes and nucleotide sequences, many of which have also been shown to afford protection when introduced into plants. The coat protein has, however, been the most widely applied, and as such has been selected to illustrate the techniques involved. Plant Virology Protocols has been divided into six major sections, c- taining 55 chapters in total.
Plant Signal Transduction
DOWNLOAD
Author : Jose R. Botella
language : en
Publisher: Humana Press
Release Date : 2016-08-23
Plant Signal Transduction written by Jose R. Botella and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
This fully updated volume reflects the spectacular advances in our knowledge of signal transduction pathways with a selection of ‘classic’ as well as newly developed approaches. These detailed approaches expand into the fields of molecular biology, biochemistry, physiology, cell biology, genetics, and genomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and up-to-date, Plant Signal Transduction: Methods and Protocols, Second Edition serves as an ideal guide for researchers exploring the vast array of signals produced by plants to ensure their survival.
Signal Transduction Protocols
DOWNLOAD
Author : Robert C. Dickson
language : en
Publisher:
Release Date : 2004
Signal Transduction Protocols written by Robert C. Dickson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Cytology categories.
Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated Signal Transduction Protocols with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class. The protocols follow the successful Methods in Molecular Biology"!series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and eminently practical, Signal Transduction Protocols, 2nd ed. offers novice and experienced researchers alike an indispensable set of standardized, but readily adaptable, approaches for the assay of key signaling molecules-an invaluable tool for all laboratories newly entering the field, expanding the breadth of previous investigations, or seeking to improve an existing approach
Human Retrovirus Protocols
DOWNLOAD
Author : Tuofu Zhu
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Human Retrovirus Protocols written by Tuofu Zhu and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.
Plant Lipid Signaling Protocols
DOWNLOAD
Author : Teun Munnik
language : en
Publisher: Springer Nature
Release Date : 2013-05-17
Plant Lipid Signaling Protocols written by Teun Munnik and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-05-17 with Science categories.
As scientist begin to understand the complexity of lipid signaling and its roles in plant biology, there is an increasing interest in their analysis. Due to the low abundancy and transient nature of some of these hydrophobic compounds, this is not always easy. In Plant Lipid Signaling Protocols, expert researchers in the field detail experimental approaches by which plant signaling lipids can be studied. These methods and techniques include analysis of plant signaling lipids, including detailed protocols to detect various relevant compounds by targeted or non-targeted approaches; to assay relevant enzyme activities in biological material or using recombinant enzymes; to test for specific binding of signaling lipids to protein partners; or to visualize signaling lipids or lipid-derived signals in living plant cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Lipid Signaling Protocols aids plant researchers in the continuing to study the roles of lipid signals.