Site Directed Insertion Of Transgenes

DOWNLOAD

Download Site Directed Insertion Of Transgenes PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Site Directed Insertion Of Transgenes book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page

Site Directed Insertion Of Transgenes

DOWNLOAD
Author : Sylvaine Renault
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-07-30

Site Directed Insertion Of Transgenes written by Sylvaine Renault and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-07-30 with Science categories.

The post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather useful information’s scattered within the literature. This book aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification. Technologies based on Zinc Finger nucleases, Meganucleases, TALEN, CRE and FLP recombinase, C31 integrase, transposases and resolvases are fully detailed with their strengths and weaknesses. All these information’s will help students and experienced researchers to understand and choose the best technology for their own purposes.

Biotechnology And Biodiversity

DOWNLOAD
Author : M. R. Ahuja
language : en
Publisher: Springer
Release Date : 2014-10-13

Biotechnology And Biodiversity written by M. R. Ahuja and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-10-13 with Science categories.

The purpose of this book is to assess the potential effects of biotechnological approaches particularly genetic modification on biodiversity and the environment. All aspects of biodiversity such as ecological diversity, species diversity and genetic diversity are considered. Higher organisms contain a specific set of linear DNA molecules called chromosomes and a complete set of chromosomes in an organism comprises its genome. The collection of traits displayed by any organism (phenotype) depends on the genes present in its genome (genotype). The appearance of any specific trait also will depend on many other factors, including whether the gene(s) responsible for the trait is/are turned on (expressed) or off, the specific cells within which the genes are expressed and how the genes, their expression and the gene products interact with environmental factors. The primary biotechnology which concerns us is that of genetic manipulation, which has a direct impact on biodiversity at the genetic level. By these manipulations, novel genes or gene fragments can be introduced into organisms (creating transgenics) or existing genes within an organism can be altered. Transgenics are a major area of concern, combining genes from different species to effectively create novel organisms. Current rates of disappearance of biological and cultural diversity in the world are unprecedented. Intensive resource exploitation due to social and economic factors has led to the destruction, conversion or degradation of ecosystems. Reversing these trends requires time to time assessment to integrate conservation and development.

Modern Tools For Genetic Engineering

DOWNLOAD
Author : Michael Kormann
language : en
Publisher: BoD – Books on Demand
Release Date : 2016-05-18

Modern Tools For Genetic Engineering written by Michael Kormann and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-18 with Science categories.

Site-specific endonucleases create double-strand breaks within the genome and can be targeted to literally any genetic mutation. Together with a repair template, a correction of the defective locus becomes possible. This book offers insight into the modern tools of genome editing, their hurdles and their huge potential. A new era of in vivo genetic engineering has begun.

Transgenic Animal Technology

DOWNLOAD
Author : Carl A. Pinkert
language : en
Publisher: Newnes
Release Date : 2014-06-17

Transgenic Animal Technology written by Carl A. Pinkert and has been published by Newnes this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-17 with Science categories.

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. - Contains a comprehensive collection of transgenic animal and gene transfer methods - Discusses background and introduction to techniques and animal systems - Teaches practical step-by-step protocols - Fully revised with updates to reflect state-of-the-art technology and associated changes to date

Omics Applications In Crop Science

DOWNLOAD
Author : Debmalya Barh
language : en
Publisher: CRC Press
Release Date : 2013-12-16

Omics Applications In Crop Science written by Debmalya Barh and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-16 with Medical categories.

Merging topical data from recently published review and research articles, as well as the knowledge and insight of industry experts, Omics Applications in Crop Science delves into plant science, and various technologies that use omics in agriculture. This book concentrates on crop breeding and environmental applications, and examines the applications of various omics technologies including genomics, transcriptomics, proteomics, metabolomics to important agronomic, horticultural, medicinal, plantation, fiber, forage, and bioenergy crops. It covers the application of omics technologies in several important crops, including cereal, and pulse. It explores the brassica species, drought tolerance in rice, and genetic engineering of the potato. The book discusses temperate fruits; and omics of medicinal plants, the metabolomics of Catharanthus roseus and how the medicinally important alkaloids of the plant are produced, as well as the omics of another important medicinal plant, Withania somnifera. It examines floriculture, the omics advances in tea, and omics strategies in improving the fiber qualities of cotton. It provides omics-related information on forest trees and forage crops, and offers a detailed account on how omics technologies are applicable in molecular farming, along with associated issues such as commercial aspects of molecular farming, clinical trials of plant-produced pharmaceuticals, regulatory issues and intellectual property rights. Written as a resource for plant biologists, plant breeders, agriculture scientists, researchers and college students studying various fields in agriculture, and the agri industries, OMICS Applications in Crop Science compiles the latest research in this essential field of modern crop and plant science utilizing various omics technologies and their applications in a number of important crops/plants from agronomy, pomology, olericulture, floriculture, medicinal plants, plantation and energy crops, agro-forestry, and more.

Chemical And Genomic Methods In Nucleic Acid Biology

DOWNLOAD
Author :
language : en
Publisher: Elsevier
Release Date : 2024-10-15

Chemical And Genomic Methods In Nucleic Acid Biology written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-10-15 with Science categories.

Chemical and Genomic Methods in Nucleic Acid Biology, Volume 704 highlights new advances in the field of nucleic acids, with this new volume presenting interesting chapters written by an international board of authors. Specific chapters in this new release include A real-time FRET-based biochemical assay for DNA deaminase enzymology and inhibition, DEER spectroscopy to probe DNA wrapping by protein complexes, PAR-dCLIP: Enhancing PhotoActivatable Ribonucleoside analog-enhanced CrossLinking and Immunoprecipitation through capture of bound 5' terminal RNA fragments, Site-specific targeting of transgene cDNA insertion, Simultaneous Profiling of the RNA Targets of Two RNA-Binding Proteins Using TRIBE-STAMP, and much more.Additional chapters cover Ensemble FRET Approach to Directly and Continuously Monitor Protein? DNA Interactions, Utilizing nuclear extracts to characterize protein-DNA interactions at the single-molecule level, RNA-Guided Protease Activation in CRISPR-Cas, Activity-based profiling of RNA modifying enzymes, Purification of Cas9 and Cas12a for Peptide-Assisted Genome Editing, Studying the intersection of nucleoside modifications and SARS-CoV-2 RNA-dependent RNA transcription using an in vitro reconstituted system, and more. - Provides the latest information on acids and biology researches - Offers outstanding and original reviews on a range of biological research topics - Serves as an indispensable reference for researchers and students alike

Transgenic Plants And Beyond

DOWNLOAD
Author :
language : en
Publisher: Academic Press
Release Date : 2018-02-14

Transgenic Plants And Beyond written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-02-14 with Science categories.

Transgenic Plants, Volume 86, the latest release in the series Advances in Botanical Research, brings together information needed by many teachers, researchers and managers who have to consider biotechnology from a scientific or legal point-of-view. It presents authors who bring their long personal experience on a given subject. Although the subjects are technical in nature, the take-home message of each chapter is understandable by non-specialists. - Encompasses various aspects of the GMO debate (its historical background, current status, recent research outcomes, potential future developments) - Written by highly competent authors from all continents - Based on facts and written in a dispassionate and non-polemical tone

Gene Therapy

DOWNLOAD
Author : Chunsheng Kang
language : en
Publisher: BoD – Books on Demand
Release Date : 2011-06-22

Gene Therapy written by Chunsheng Kang and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-22 with Medical categories.

The aim of this book is to cover key aspects of existing problems in the field of development and future perspectives in gene therapy. Contributions consist of basic and translational research, as well as clinical experiences, and they outline functional mechanisms, predictive approaches, patient-related studies and upcoming challenges in this stimulating but also controversial field of gene therapy research. This source will make our doctors become comfortable with the common problems of gene therapy and inspire others to delve a bit more deeply into a topic of interest.

Laboratory Fish In Biomedical Research

DOWNLOAD
Author : Livia D'Angelo
language : en
Publisher: Academic Press
Release Date : 2021-08-28

Laboratory Fish In Biomedical Research written by Livia D'Angelo and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-08-28 with Science categories.

Fish, and particularly zebrafish, have become the fastest-growing segment of the research population. They offer several advantages, in terms of biology and technologies to apply, and thus are employed in numerous research fields. Laboratory Fish in Biomedical Research: Biology, Husbandry and Research Applications for Zebrafish, Medaka, Killifish, Swordtail Fish, Cavefish, Stickleback, Goldfish and Danionella Translucida addresses the relevant and increasing need to collect cutting-edge knowledge on husbandry, maintenance, welfare and experimental protocols of the most common freshwater species under standard laboratory conditions. - Provides husbandry and management protocols, devices and water systems - Shows strength and weakness of breeding - Explores potential scientific applications and experimental protocols. with regards to the most used freshwater fish used for scientific purposes

Crispr Cas In Agriculture Opportunities And Challenges

DOWNLOAD
Author : Sandeep Kumar
language : en
Publisher: Frontiers Media SA
Release Date : 2021-05-28

Crispr Cas In Agriculture Opportunities And Challenges written by Sandeep Kumar and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-05-28 with Science categories.