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Size Exclusion Chromatography System For Macromolecular Interaction Analysis


Size Exclusion Chromatography System For Macromolecular Interaction Analysis
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Size Exclusion Chromatography System For Macromolecular Interaction Analysis


Size Exclusion Chromatography System For Macromolecular Interaction Analysis
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Author :
language : en
Publisher:
Release Date : 1988

Size Exclusion Chromatography System For Macromolecular Interaction Analysis written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with categories.


A low pressure, microcomputer controlled system employing high performance liquid chromatography (HPLC) allows for precise analysis of the interaction of two reversibly associating macromolecules such as proteins. Since a macromolecular complex migrates faster than its components during size-exclusion chromatography, the difference between the elution profile of a mixture of two macromolecules and the summation of the elution profiles of the two components provides a quantifiable indication of the degree of molecular interaction. This delta profile is used to qualitatively reveal the presence or absence of significant interaction or to rank the relative degree of interaction in comparing samples and, in combination with a computer simulation, is further used to quantify the magnitude of the interaction in an arrangement wherein a microcomputer is coupled to analytical instrumentation in a novel manner.



Hplc Of Biological Macro Molecules Revised And Expanded


Hplc Of Biological Macro Molecules Revised And Expanded
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Author : Karen M. Gooding
language : en
Publisher: CRC Press
Release Date : 2002-01-08

Hplc Of Biological Macro Molecules Revised And Expanded written by Karen M. Gooding and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-01-08 with Science categories.


Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.



Handbook Of Size Exclusion Chromatography And Related Techniques


Handbook Of Size Exclusion Chromatography And Related Techniques
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Author : Chi-San Wu
language : en
Publisher: CRC Press
Release Date : 2003-11-04

Handbook Of Size Exclusion Chromatography And Related Techniques written by Chi-San Wu and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-11-04 with Science categories.


Documenting critical advances in this rapidly evolving field, the Second Edition highlights the need for new applications and technologies that assist in the determination of molecular weight and molecular weight distributions of polymers in an accurate, efficient manner. This volume presents the latest findings from a international team of specialists and continues to inspire and extend practical applications of size exclusion chromatography (SEC). It includes six new chapters covering high-speed size exclusion chromatography, SEC of low molecular weight materials, and the extended family of techniques, from two-dimensional liquid chromatography to high osmotic pressure chromatography.



Size Exclusion Chromatography


Size Exclusion Chromatography
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Author : Barry J. Hunt
language : en
Publisher: Blackie Academic and Professional
Release Date : 1989

Size Exclusion Chromatography written by Barry J. Hunt and has been published by Blackie Academic and Professional this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989 with Science categories.




Biological Small Angle Scattering Techniques Strategies And Tips


Biological Small Angle Scattering Techniques Strategies And Tips
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Author : Barnali Chaudhuri
language : en
Publisher: Springer
Release Date : 2017-12-07

Biological Small Angle Scattering Techniques Strategies And Tips written by Barnali Chaudhuri and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-07 with Science categories.


This book provides a clear, comprehensible and up-to-date description of how Small Angle Scattering (SAS) can help structural biology researchers. SAS is an efficient technique that offers structural information on how biological macromolecules behave in solution. SAS provides distinct and complementary data for integrative structural biology approaches in combination with other widely used probes, such as X-ray crystallography, Nuclear magnetic resonance, Mass spectrometry and Cryo-electron Microscopy. The development of brilliant synchrotron small-angle X-ray scattering (SAXS) beam lines has increased the number of researchers interested in solution scattering. SAS is especially useful for studying conformational changes in proteins, highly flexible proteins, and intrinsically disordered proteins. Small-angle neutron scattering (SANS) with neutron contrast variation is ideally suited for studying multi-component assemblies as well as membrane proteins that are stabilized in surfactant micelles or vesicles. SAS is also used for studying dynamic processes of protein fibrillation in amyloid diseases, and pharmaceutical drug delivery. The combination with size-exclusion chromatography further increases the range of SAS applications. The book is written by leading experts in solution SAS methodologies. The principles and theoretical background of various SAS techniques are included, along with practical aspects that range from sample preparation to data presentation for publication. Topics covered include techniques for improving data quality and analysis, as well as different scientific applications of SAS. With abundant illustrations and practical tips, we hope the clear explanations of the principles and the reviews on the latest progresses will serve as a guide through all aspects of biological solution SAS. The scope of this book is particularly relevant for structural biology researchers who are new to SAS. Advanced users of the technique will find it helpful for exploring the diversity of solution SAS methods and applications. Chapter 3 of this book is available open access under a CC BY 4.0 license at link.springer.com.



Detection And Data Analysis In Size Exclusion Chromatography


Detection And Data Analysis In Size Exclusion Chromatography
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Author : Theodore Provder
language : en
Publisher:
Release Date : 1987

Detection And Data Analysis In Size Exclusion Chromatography written by Theodore Provder and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987 with Science categories.




Hplc Of Biological Macro Molecules Revised And Expanded


Hplc Of Biological Macro Molecules Revised And Expanded
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Author : Karen M. Gooding
language : en
Publisher: CRC Press
Release Date : 2002-01-08

Hplc Of Biological Macro Molecules Revised And Expanded written by Karen M. Gooding and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-01-08 with Science categories.


Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.



Modern Size Exclusion Liquid Chromatography


Modern Size Exclusion Liquid Chromatography
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Author : Andre Striegel
language : en
Publisher: John Wiley & Sons
Release Date : 2009-07-31

Modern Size Exclusion Liquid Chromatography written by Andre Striegel and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-07-31 with Science categories.


The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique. Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. This Second Edition brings the book thoroughly up to date, with expert coverage of: New and emerging industrial and research applications Practical aspects of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC technologies for polymer architecture and copolymer analysis Updated information on the latest equipment and techniques New best practices for the lab SEC in relation to polymer characterization techniques such as GPEC, LCCC, and rheology Throughout the text, detailed examples guide you step by step through all the latest techniques and applications. With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods.



Hplc Made To Measure


Hplc Made To Measure
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Author : Stavros Kromidas
language : en
Publisher: John Wiley & Sons
Release Date : 2008-06-05

Hplc Made To Measure written by Stavros Kromidas and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-06-05 with Science categories.


The only topical HPLC book to focus on optimization, this volume addresses the needs of HPLC users who wish to constantly improve their methods, in particular in terms of throughput, accuracy and cost-effectiveness. This handbook features contributions from such bestselling authors as John W. Dolan, Michael McBrien, Veronika R. Meyer, Uwe D. Neue, Lloyd R. Snyder, and Klaus K. Unger, as well as from scientists working for major companies, including Agilent, AstraZeneca, Merck, Schering, Tosoh Biosep, VWR, and Waters. It covers essential aspects of optimization in general, optimization in different LC-modi, hyphenated techniques and computer-aided optimization. The whole is rounded off with a section of user reports.



Official Gazette Of The United States Patent And Trademark Office


Official Gazette Of The United States Patent And Trademark Office
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Author : United States. Patent and Trademark Office
language : en
Publisher:
Release Date : 1988-08

Official Gazette Of The United States Patent And Trademark Office written by United States. Patent and Trademark Office and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988-08 with Patents categories.