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Small Angle Scattering Part A Methods For Structural Investigation


Small Angle Scattering Part A Methods For Structural Investigation
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Small Angle Scattering Part A Methods For Structural Investigation


Small Angle Scattering Part A Methods For Structural Investigation
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Author :
language : en
Publisher: Academic Press
Release Date : 2022-11-17

Small Angle Scattering Part A Methods For Structural Investigation written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-11-17 with Science categories.


Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications. Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more.



Small Angle Scattering Part A Methods For Structural Investigation


Small Angle Scattering Part A Methods For Structural Investigation
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Author :
language : en
Publisher: Elsevier
Release Date : 2022-10-15

Small Angle Scattering Part A Methods For Structural Investigation written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-10-15 with Science categories.


Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications. Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on Small Angle Scattering: Methods for Structural Investigation



Biological Small Angle Scattering Techniques Strategies And Tips


Biological Small Angle Scattering Techniques Strategies And Tips
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Author : Barnali Chaudhuri
language : en
Publisher: Springer
Release Date : 2017-12-07

Biological Small Angle Scattering Techniques Strategies And Tips written by Barnali Chaudhuri and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-07 with Science categories.


This book provides a clear, comprehensible and up-to-date description of how Small Angle Scattering (SAS) can help structural biology researchers. SAS is an efficient technique that offers structural information on how biological macromolecules behave in solution. SAS provides distinct and complementary data for integrative structural biology approaches in combination with other widely used probes, such as X-ray crystallography, Nuclear magnetic resonance, Mass spectrometry and Cryo-electron Microscopy. The development of brilliant synchrotron small-angle X-ray scattering (SAXS) beam lines has increased the number of researchers interested in solution scattering. SAS is especially useful for studying conformational changes in proteins, highly flexible proteins, and intrinsically disordered proteins. Small-angle neutron scattering (SANS) with neutron contrast variation is ideally suited for studying multi-component assemblies as well as membrane proteins that are stabilized in surfactant micelles or vesicles. SAS is also used for studying dynamic processes of protein fibrillation in amyloid diseases, and pharmaceutical drug delivery. The combination with size-exclusion chromatography further increases the range of SAS applications. The book is written by leading experts in solution SAS methodologies. The principles and theoretical background of various SAS techniques are included, along with practical aspects that range from sample preparation to data presentation for publication. Topics covered include techniques for improving data quality and analysis, as well as different scientific applications of SAS. With abundant illustrations and practical tips, we hope the clear explanations of the principles and the reviews on the latest progresses will serve as a guide through all aspects of biological solution SAS. The scope of this book is particularly relevant for structural biology researchers who are new to SAS. Advanced users of the technique will find it helpful for exploring the diversity of solution SAS methods and applications. Chapter 3 of this book is available open access under a CC BY 4.0 license at link.springer.com.



Small Angle Scattering Part A Methods For Structural Investigation


Small Angle Scattering Part A Methods For Structural Investigation
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Author :
language : en
Publisher: Academic Press
Release Date : 2022-11-19

Small Angle Scattering Part A Methods For Structural Investigation written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-11-19 with Science categories.


Small Angle Scattering, Part A: Methods for Structural Investigation, Volume 675 in the Methods in Enzymology series, highlights new advances in the field, with new chapters in this updated release including SAXS foundations and metrics, Contrast variation sample preparation protocols, experimental procedures, and rudimentary analysis, Molecular deuteration for neutron scattering, Planning, Executing and Assessing the Feasibility of SANS Contrast Variation Experiments, Technical considerations for small-angle neutron scattering from biological macromolecules, and Advanced sample environments and capabilities at our synchrotron X-ray beamline with example applications. Additional sections in the book cover SEC-SAXS-MALS data acquisition and processing pipeline at SIBYLS, SEC-SAXS: pros and cons, experimental set-up, examples and software developments, Radiation damage and sample economy for stopped-flow methods in the time regime of millisecond and above, Stopped-flow-time-resolved SAXS, Insights on Temp-jump, time-resolved SAXS, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on Small Angle Scattering: Methods for Structural Investigation



Structural Investigations Using Small Angle Scattering Techniques And Contrast Variation


Structural Investigations Using Small Angle Scattering Techniques And Contrast Variation
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Author :
language : en
Publisher:
Release Date : 2014

Structural Investigations Using Small Angle Scattering Techniques And Contrast Variation written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.




Structure Analysis By Small Angle X Ray And Neutron Scattering


Structure Analysis By Small Angle X Ray And Neutron Scattering
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Author : L.A. Feigin
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11

Structure Analysis By Small Angle X Ray And Neutron Scattering written by L.A. Feigin and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.


Small-angle scattering of X rays and neutrons is a widely used diffraction method for studying the structure of matter. This method of elastic scattering is used in various branches of science and technology, includ ing condensed matter physics, molecular biology and biophysics, polymer science, and metallurgy. Many small-angle scattering studies are of value for pure science and practical applications. It is well known that the most general and informative method for investigating the spatial structure of matter is based on wave-diffraction phenomena. In diffraction experiments a primary beam of radiation influences a studied object, and the scattering pattern is analyzed. In principle, this analysis allows one to obtain information on the structure of a substance with a spatial resolution determined by the wavelength of the radiation. Diffraction methods are used for studying matter on all scales, from elementary particles to macro-objects. The use of X rays, neutrons, and electron beams, with wavelengths of about 1 A, permits the study of the condensed state of matter, solids and liquids, down to atomic resolution. Determination of the atomic structure of crystals, i.e., the arrangement of atoms in a unit cell, is an important example of this line of investigation.



Scattering Methods In Structural Biology Part B


Scattering Methods In Structural Biology Part B
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Author :
language : en
Publisher: Elsevier
Release Date : 2023-01-20

Scattering Methods In Structural Biology Part B written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-01-20 with Science categories.


Scattering Methods in Structural Biology, Part B, Volume 676 in the Methods in Enzymology serial, highlights advances in the field, presenting chapters on Quality controls, Refining biomolecular structures and ensembles by SAXS-driven molecular dynamics simulations, Data analysis and modelling of small-angle scattering data with contrast variation, Observing protein degradation in solution by the PAN-20S proteasome complex: state-of-the-art and future perspectives of TR-SANS as a complementary tool to NMR, crystallography and Cryo-EM, Extracting structural insights from chemically-specific soft X-ray scattering, Reconstruction of 3D density of biological macromolecules from solution scattering, ATSAS- present state and new developments in computational methods, and much more. Additional chapters cover Modeling Structure and Dynamics of Protein Complexes with SAXS Profiles (FoXSDock and MultiFoXS), Validation of macromolecular flexibility in solution by SAXS, Combining NMR, SAXS and SANS to characterize the structure and dynamics of protein complexes, Application of Molecular Simulation Methods to Analyze SAS Data, and more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology serial Updated release includes the latest information on Small Angle Scattering Methods for Structural Interpretation



Small Angle X Ray And Neutron Scattering From Solutions Of Biological Macromolecules


Small Angle X Ray And Neutron Scattering From Solutions Of Biological Macromolecules
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Author : Dmitri I. Svergun
language : en
Publisher: Oxford University Press, USA
Release Date : 2020-01-06

Small Angle X Ray And Neutron Scattering From Solutions Of Biological Macromolecules written by Dmitri I. Svergun and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-01-06 with Science categories.


This book describes all aspects of the technique of small-angle scattering of X-rays and neutrons, including instrumentation, sample requirements, data interpretation and modelling methods, in a comprehensive way and gives examples of applications in various fields of biophysics and biochemistry.



Small Angle Scattering


Small Angle Scattering
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Author : Ian W. Hamley
language : en
Publisher: John Wiley & Sons
Release Date : 2021-04-19

Small Angle Scattering written by Ian W. Hamley and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-04-19 with Technology & Engineering categories.


SMALL-ANGLE SCATTERING A comprehensive and timely volume covering contemporary research, practical techniques, and theoretical approaches to SAXS and SANS Small-Angle Scattering: Theory, Instrumentation, Data, and Applications provides authoritative coverage of both small-angle X-ray scattering (SAXS), small-angle neutron scattering (SANS) and grazing incidence small-angle scattering (GISAS) including GISAXS and GISANS. This single-volume resource offers readers an up-to-date view of the state of the field, including the theoretical foundations, experimental methods, and practical applications of small-angle scattering (SAS) techniques including laboratory and synchrotron SAXS and reactor/spallation SANS. Organized into six chapters, the text first describes basic theory, instrumentation, and data analysis. The following chapters contain in-depth discussion on various applications of SAXS and SANS and GISAXS and GISANS, and on specific techniques for investigating structure and order in soft materials, biomolecules, and inorganic and magnetic materials. Author Ian Hamley draws from his more than thirty years’ experience working with many systems, instruments, and types of small-angle scattering experiments across most European facilities to present the most complete introduction to the field available. This book: Presents uniquely broad coverage of practical and theoretical approaches to SAXS and SANS Includes practical information on instrumentation and data analysis Offers useful examples and an accessible and concise presentation of topics Covers new developments in the techniques of SAXS and SANS, including GISAXS and GISANS Small-Angle Scattering: Theory, Instrumentation, Data, and Applications is a valuable source of detailed information for researchers and postgraduate students in the field, as well as other researchers using X-ray and neutron scattering to investigate soft materials, other nanostructured materials and biomolecules such as proteins.



Small Angle Scattering Neutrons X Rays Light From Complex Systems


Small Angle Scattering Neutrons X Rays Light From Complex Systems
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Author : Eugen Mircea Anitas
language : en
Publisher: Springer
Release Date : 2019-08-08

Small Angle Scattering Neutrons X Rays Light From Complex Systems written by Eugen Mircea Anitas and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-08 with Science categories.


This book addresses the basic physical phenomenon of small-angle scattering (SAS) of neutrons, x-rays or light from complex hierarchical nano- and micro-structures. The emphasis is on developing theoretical models for the material structure containing self-similar or fractal clusters. Within the suggested framework, key approaches for extracting structural information from experimental scattering data are investigated and presented in detail. The range of parameters which can be obtained pave the road towards a better understanding of the correlations between geometrical and various physical properties (electrical, magnetic, mechanical, optical, dynamical, transport etc.) in fractal nano- and micro-materials.