Spectral And Imaging Cytometry
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Spectral And Imaging Cytometry
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Author : Natasha S. Barteneva
language : en
Publisher: Springer Nature
Release Date : 2023-04-19
Spectral And Imaging Cytometry written by Natasha S. Barteneva and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-04-19 with Science categories.
This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations. Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field. Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Imaging Flow Cytometry
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Author : Natasha S. Barteneva
language : en
Publisher: Humana
Release Date : 2015-11-23
Imaging Flow Cytometry written by Natasha S. Barteneva and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-11-23 with Medical categories.
This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.
Spectral Imaging
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Author :
language : en
Publisher:
Release Date : 2005
Spectral Imaging written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Diagnostic imaging categories.
Understanding Light Microscopy
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Author : Jeremy Sanderson
language : en
Publisher: John Wiley & Sons
Release Date : 2019-03-28
Understanding Light Microscopy written by Jeremy Sanderson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-28 with Science categories.
Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.
Recent Advances In Cytometry
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Author : Zbigniew Darzynkiewicz
language : en
Publisher: Academic Press
Release Date : 2011
Recent Advances In Cytometry written by Zbigniew Darzynkiewicz and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Cell Separation categories.
Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay. Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays * Discusses many new assays developed since the previous edition. Presents recent developments in cytometric intrumentation/technology.
Recent Advances In Cytometry Part A
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Author :
language : en
Publisher: Academic Press
Release Date : 2011-06-13
Recent Advances In Cytometry Part A written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-13 with Science categories.
Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay. - Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays - Discusses many new assays developed since the previous edition - Presents recent developments in cytometric intrumentation/technology
Advanced Optical Flow Cytometry
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Author : Valery V. Tuchin
language : en
Publisher: John Wiley & Sons
Release Date : 2011-03-31
Advanced Optical Flow Cytometry written by Valery V. Tuchin and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-03-31 with Science categories.
A detailed look at the latest research in non-invasive in vivo cytometry and its applications, with particular emphasis on novel biophotonic methods, disease diagnosis, and monitoring of disease treatment at single cell level in stationary and flow conditions. This book thus covers the spectrum ranging from fundamental interactions between light, cells, vascular tissue, and cell labeling particles, to strategies and opportunities for preclinical and clinical research. General topics include light scattering by cells, fast video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic methods for cellular diagnostics and monitoring of disease treatment in living organisms. Also presented are discussions of advanced methods and techniques of classical flow cytometry.
Biomedical Optical Imaging
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Author : James G. Fujimoto
language : en
Publisher: Oxford University Press
Release Date : 2009-04-22
Biomedical Optical Imaging written by James G. Fujimoto and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-04-22 with Science categories.
Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.
Handbook Of Biological Confocal Microscopy
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Author : James Pawley
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-06-02
Handbook Of Biological Confocal Microscopy written by James Pawley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-06-02 with Science categories.
Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.
Flow Cytometry
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Author : Ingrid Schmid
language : en
Publisher: BoD – Books on Demand
Release Date : 2016-08-24
Flow Cytometry written by Ingrid Schmid and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-24 with Medical categories.
Flow cytometry - Select Topics is a collection of chapters that illustrate the constantly evolving application of flow cytometry to diverse areas of research or clinical investigations. It includes chapters on the utilization of flow cytometry in the fields of human reproduction and fertility, platelet function, apoptosis, inflammation research, leukemia immunophenotyping, and transplantation.