Stem Cell Tools And Other Experimental Protocols
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Stem Cell Tools And Other Experimental Protocols
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Author :
language : en
Publisher: Elsevier
Release Date : 2006-12-12
Stem Cell Tools And Other Experimental Protocols written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-12-12 with Technology & Engineering categories.
This is the third of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a "cookbook and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using "conventional and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories. - Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering - Presents the latest most innovative technologies - Addresses therapeutic relevance including FDA compliance and tissue engineering
Stem Cell Tools And Other Experimental Protocols
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Author :
language : en
Publisher:
Release Date :
Stem Cell Tools And Other Experimental Protocols written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Human Pluripotent Stem Cells
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Author : Philip H. Schwartz
language : en
Publisher: Humana Press
Release Date : 2011-08-09
Human Pluripotent Stem Cells written by Philip H. Schwartz and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-09 with Science categories.
Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments. Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in Human Pluripotent Stem Cells: Methods and Protocols, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Human Pluripotent Stem Cells: Methods and Protocols serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.
Essential Stem Cell Methods
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Author : Robert Lanza
language : en
Publisher: Academic Press
Release Date : 2011-10-13
Essential Stem Cell Methods written by Robert Lanza and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-13 with Science categories.
This is a fast-moving field, and these detailed methods will help drive advances in stem cell research. The editors have hand selected step-by-step methods from researchers with extensive reputations and expertise.This volume, as part of the Reliable Lab Solutions series, delivers busy researchers a handy, time-saving source for the best methods and protocols in stem cells. - Provides powerful research opportunities for those interested in perusing work in pluripotent stem cells, disease modeling, and other aspects of basic stem cell research - Refines, organizes and updates popular methods from flagship series, Methods in Enzymology - Highlights top downloads, enhanced with author tips and tricks and pitfalls to avoid
Hydrogen Peroxide And Cell Signaling Part C
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Author :
language : en
Publisher: Academic Press
Release Date : 2013-07-11
Hydrogen Peroxide And Cell Signaling Part C written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-07-11 with Science categories.
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the third of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers hydrogen peroxide and cell signaling - Contains chapters on such topics as the biological chemistry of hydrogen peroxide, reactive oxygen species in the activation of MAP kinases, and investigating the role of reactive oxygen species in regulating autophagy
Hydrogen Peroxide And Cell Signaling Part A
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Author :
language : en
Publisher: Academic Press
Release Date : 2013-06-19
Hydrogen Peroxide And Cell Signaling Part A written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-19 with Science categories.
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers hydrogen peroxide and cell signaling - Contains chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer
Guide To Techniques In Mouse Development Part A
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-08-04
Guide To Techniques In Mouse Development Part A written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-08-04 with Science categories.
Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. - Update of volume 225 of Methods in Enzymology, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Covers new technologies and methodologies, including: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals - use of gene-trap mutagenesis - analysis of allele-specific expresion - use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics
The Unfolded Protein Response And Cellular Stress
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Author : P. Michael Conn
language : en
Publisher: Academic Press
Release Date : 2011
The Unfolded Protein Response And Cellular Stress written by P. Michael Conn and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Cell physiology categories.
This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological ins.
The Unfolded Protein Response And Cellular Stress Part C
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Author :
language : en
Publisher: Academic Press
Release Date : 2011-02-14
The Unfolded Protein Response And Cellular Stress Part C written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-02-14 with Science categories.
This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights - This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation - The authors explain how these methods are able to provide important biological insights
The Unfolded Protein Response And Cellular Stress Part B
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Author : P. Michael Conn
language : en
Publisher: Academic Press
Release Date : 2011-01-22
The Unfolded Protein Response And Cellular Stress Part B written by P. Michael Conn and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-01-22 with Science categories.
This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. - This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation - The authors explain how these methods are able to provide important biological insights