Structural Studies On The Plant Plasma Membrane Calcium Atpase
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Structural Studies On The Plant Plasma Membrane Calcium Atpase
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Author : Swati Basu
language : en
Publisher:
Release Date : 1994
Structural Studies On The Plant Plasma Membrane Calcium Atpase written by Swati Basu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with categories.
The plasma membrane Ca$sp{2+}$-ATPase is a ubiquitous membrane protein that mediates the active transport of calcium out of the cytoplasm into the cell exterior. In plant cells it is believed to play a major regulatory role in signal transduction by helping to maintain a large gradient of calcium across the plasma membrane, thereby keeping the cell poised for response to environmental and hormonal cues. Research using isolated membrane vesicles has resulted in the study of the plasma membrane Ca$sp{2+}$-ATPase, most of which has focused on characterization of its calcium transport properties. In this study a biochemical approach was taken to examine the essential amino acid residues which may be involved in the mechanism of the enzyme. For this, chemical modification reagents were used. In addition radiation inactivation analysis was used to examine the quaternary structure of the enzyme. The study of the plant plasma membrane Ca$sp{2+}$-ATPase was conducted using vesicles isolated from red beet (Beta vulgaris L.) storage tissue which provided a means to examine the protein in its native state in the membrane. This work was conducted in order to have a better understanding of the function of the plasma membrane Ca$sp{2+}$-ATPase and thereby establish structure-function relationships. In this investigation, chemical modification reagents such as phenylglyoxal and 2,3-butanedione were used to determine whether arginine residues are involved in the mechanism of the plasma membrane Ca$sp{2+}$-ATPase. In addition, the reagents diethylpyrocarbonate, n-ethylmaleimide and erythrosin isothiocyanate were used to determine whether histidine, cysteine and lysine residues are involved in the mechanism of the enzyme respectively. All reagents inhibited both transport and hydrolytic properties of the enzyme. Therefore the results indicate that the above residues are involved in the activity of the enzyme, of which arginine residues derivitized by the reagents indicated, are probably located at or near the active site. Radiation inactivation analysis studies on the red beet plasma membrane Ca$sp{2+}$-ATPase resulted in the determination of the target molecular size of the enzyme to be 245 kDa, and that the enzyme may function as a dimer in its native state. Results from this study provide information for further research on the structure of the plant plasma membrane Ca$sp{2+}$-ATPase and the establishment of its role in signal transduction in plants.
Structural Studies On The Plant Plasma Membrane Ca2 Atpase
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Author : Swati Basu
language : en
Publisher:
Release Date : 1994
Structural Studies On The Plant Plasma Membrane Ca2 Atpase written by Swati Basu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with categories.
The Plant Plasma Membrane
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Author : Christer Larsson
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
The Plant Plasma Membrane written by Christer Larsson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The plasma membrane forms the living barrier between the cell and its surroundings. For this reason it has a wide range of important functions related to the regulation of the composition of the cell interior and to com munication with the cell exterior. The plasma membrane has therefore attracted a lot of research interest. Until the early 1970's it was only pos sible to study the plasma membrane in situ, its structure e. g. by electron microscopy and its function e. g. by uptake of radioactively labeled com pounds into the intact cell or tissue. The first isolation of plant protoplasts by enzymatic digestion of the cell wall in the early 1970's was an important step forward in that it provided direct access to the outer surface of the plasma membrane. More importantly, T. K. Hodges and R. J. Leonard in 1972 published the description of a method by which a fraction enriched in plasma membranes could be isolated from plant tissues using sucrose gradient centrifugation. As a result, the 1970's saw a leap forward in our understanding of the structurc and function of the plasma membrane. In 1981, S. Widell and C. Larsson published the first of a series of papers in which plasma membrane vesicles of high yield and purity were isolated from a wide range of plant tissues using aqueous polymer two-phase parti tioning.
Transport And Receptor Proteins Of Plant Membranes
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Author : D.T. Clarkson
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Transport And Receptor Proteins Of Plant Membranes written by D.T. Clarkson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Mechanism And Structure Of The Plant Plasma Membrane Ca Sup 2 Atpase
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Author :
language : en
Publisher:
Release Date : 1993
Mechanism And Structure Of The Plant Plasma Membrane Ca Sup 2 Atpase written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with categories.
Objectives of this project were the following: development of an enriched preparation of the red beet plasma membrane Ca{sup 2+} ATPase in order to develop a procedure for detergent solubilization of the enzyme from the membrane using detergents, resolution by a method which could be upscaled for batch isolation, and then reconstitution into liposomes to allow characterization of Ca{sup 2+} transport by the purified enzyme and; characterization of the reaction mechanism for the coupling of nucleoside triphosphate hydrolysis to Ca{sup 2+} transport as mediated by the plasma membrane Ca{sup 2+} ATPase.
Encyclopedia Of Signaling Molecules
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Author :
language : en
Publisher:
Release Date : 19??
Encyclopedia Of Signaling Molecules written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 19?? with categories.
Ion Motive Atpases
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Author : Antonio Scarpa
language : en
Publisher:
Release Date : 1992
Ion Motive Atpases written by Antonio Scarpa and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Science categories.
The large body of recent knowledge that has allowed the recent discovery of new ATPases and the partial clarification of structural and functional aspects of the already known ATPases are the subjects of this volume. A dozen ION-MOTIVE ATPases are discussed in terms of structure, site modification, catalysis, expression, hormonal and metabolic regulation and pharmacologic intervention.
Molecular Biology Of The Cell
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Author : Bruce Alberts
language : en
Publisher:
Release Date : 2002
Molecular Biology Of The Cell written by Bruce Alberts and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Cytology categories.
Abiotic Stress Response In Plants
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Author : Arun Shanker
language : en
Publisher: BoD – Books on Demand
Release Date : 2011-08-29
Abiotic Stress Response In Plants written by Arun Shanker and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-08-29 with Science categories.
Plants, unlike animals, are sessile. This demands that adverse changes in their environment are quickly recognized, distinguished and responded to with suitable reactions. Drought, heat, cold and salinity are among the major abiotic stresses that adversely affect plant growth and productivity. In general, abiotic stress often causes a series of morphological, physiological, biochemical and molecular changes that unfavorably affect plant growth, development and productivity. Drought, salinity, extreme temperatures (cold and heat) and oxidative stress are often interrelated; these conditions singularly or in combination induce cellular damage. To cope with abiotic stresses, of paramount significance is to understand plant responses to abiotic stresses that disturb the homeostatic equilibrium at cellular and molecular level in order to identify a common mechanism for multiple stress tolerance. This multi authored edited compilation attempts to put forth an all-inclusive biochemical and molecular picture in a systems approach wherein mechanism and adaptation aspects of abiotic stress are dealt with. The chief objective of the book hence is to deliver state of the art information for comprehending the effects of abiotic stress in plants at the cellular level.
Metal Transporters
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Author : Jose M. Arguello
language : en
Publisher: Academic Press
Release Date : 2012-10-25
Metal Transporters written by Jose M. Arguello and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-10-25 with Science categories.
This volume of Current Topics in Membranes focuses on metal transmembrane transporters and pumps, a recently discovered family of membrane proteins with many important roles in the physiology of living organisms. The book summarizes the most recent advances in the field of metal ion transport and provides a broad overview of the major classes of transporters involved in homeostasis of heavy metals. Various families of the transporters and metal specificities are discussed with the focus on the structural and mechanistic aspects of their function and regulation. The reader will access information obtained through a variety of approaches ranging from X-ray crystallography to cell biology and bioinformatics, which have been applied to transporters identified in diverse biological systems, such as pathogenic bacteria, plants, humans and others. Field is cutting-edge and a lot of the information is new to research community Wide breadth of topic coverage Contributors of high renown and expertise