Structure And Function Of The Green Fluorescent Protein
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Structure And Function Of The Green Fluorescent Protein
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Author : Gerold Bublitz
language : en
Publisher:
Release Date : 1997
Structure And Function Of The Green Fluorescent Protein written by Gerold Bublitz and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with categories.
Green Fluorescent Protein
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Author : Martin Chalfie
language : en
Publisher: John Wiley & Sons
Release Date : 2005-11-18
Green Fluorescent Protein written by Martin Chalfie and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-11-18 with Science categories.
Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications. This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
Green Fluorescent Proteins
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Author : American Society for Cell Biology
language : en
Publisher: Gulf Professional Publishing
Release Date : 1999
Green Fluorescent Proteins written by American Society for Cell Biology and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Medical categories.
This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods. Key Features * Only volume on Green Fluorescent Protein research * Covers all aspects of GFP * Provides specific microscopic and data analysis methods * Discusses the design and construction of GFP fusion proteins * Covers GFP expression in animals, insects, plants, and microbes * Details procedures for time lapse imaging of living cells * Explains how to implement single molecule fluorescence detection with GFP * Discusses dual label GFP strategies for multicolor fluorescence * Presents fluorescence resonance energy transfer methods with GFPs * Details quantitative fluorescence imaging techniques * Extensively illustrated with color photographs
Imaging Living Cells
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Author : Rosario Rizzuto
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Imaging Living Cells written by Rosario Rizzuto and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Intended for bench-top use, this lab manual is suitable for both scientists and graduate students, since it combines an update on the most advanced imaging procedures with detailed protocols. Examples, carefully selected from the wide repertoire of cell pyhsiology, cover such different functional aspects as distribution of multiple ions, electrical activity, exo-endocytosis, gene expression, and the cell cycle.
Fluorescent Proteins Ii
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Author : Gregor Jung
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-01-05
Fluorescent Proteins Ii written by Gregor Jung and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-01-05 with Science categories.
Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008. Fluorescent Proteins II highlights the physicochemical and biophysical aspects of fluorescent protein technology beyond imaging. It is tailored to meet the needs of physicists, chemists and biologists who are interested in the fundamental properties of fluorescent proteins, while also focussing on specific applications. The implementations described are cutting-edge studies and exemplify how the physical and chemical properties of fluorescent proteins can stimulate novel findings in life sciences.
Protein Structure And Function
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Author : Gregory A. Petsko
language : en
Publisher: New Science Press
Release Date : 2004
Protein Structure And Function written by Gregory A. Petsko and has been published by New Science Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Medical categories.
Each title in the 'Primers in Biology' series is constructed on a modular principle that is intended to make them easy to teach from, to learn from, and to use for reference.
Make Life Visible
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Author : Yoshiaki Toyama
language : en
Publisher: Springer Nature
Release Date : 2019-10-02
Make Life Visible written by Yoshiaki Toyama and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-02 with Medical categories.
This open access book describes marked advances in imaging technology that have enabled the visualization of phenomena in ways formerly believed to be completelyimpossible. These technologies have made major contributions to the elucidation of the pathology of diseases as well as to their diagnosis and therapy. The volume presents various studies from molecular imaging to clinical imaging. It also focuses on innovative, creative, advanced research that gives full play to imaging technology inthe broad sense, while exploring cross-disciplinary areas in which individual research fields interact and pursuing the development of new techniques where they fuse together. The book is separated into three parts, the first of which addresses the topic of visualizing and controlling molecules for life. Th e second part is devoted to imaging of disease mechanisms, while the final part comprises studies on the application of imaging technologies to diagnosis and therapy. Th e book contains the proceedings of the 12th Uehara International Symposium 2017, “Make Life Visible” sponsored by the Uehara Memorial Foundation and held from June 12 to 14, 2017. It is written by leading scientists in the field and is an open access publication under a CC BY 4.0 license.
Fluorescent Proteins
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Author : Kevin F. Sullivan
language : en
Publisher: Elsevier
Release Date : 2007-12-14
Fluorescent Proteins written by Kevin F. Sullivan and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-14 with Science categories.
This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross correlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.
Illuminating Disease
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Author : Marc Zimmer
language : en
Publisher: Oxford University Press
Release Date : 2015-01-05
Illuminating Disease written by Marc Zimmer and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-01-05 with Science categories.
Green fluorescent proteins have been floating in the ocean for more than 160 million years, but it took a curious scientist, fascinated by pinpricks of green light, to begin unlocking their potential. Now these jellyfish proteins have become one of the most important tools available to researchers in modern medicine and biology. By using them to illuminate other proteins that were previously invisible even under a microscope, scientists are now able to observe facets of disease that would have otherwise gone undetected. Green fluorescent proteins are used in over three million experiments a year and have proved invaluable for tasks such as tracking HIV, breeding bird flu-resistant chickens, and confirming the existence of cancerous stem cells. In Illuminating Disease, Marc Zimmer introduces us to these revolutionary proteins, acquainting readers both with the researchers responsible for the proteins' discovery as well as their wide utility. The book details the history of genetically modified fluorescent parasites and viruses, which provide scientists with lifesaving information about the spread of diseases. Green fluorescent proteins allow scientists and doctors to understand diseases better by quite literally illuminating various microscopic interactions occurring in living cells that otherwise would have gone unseen. The book is richly illustrated, showing the visually striking uses of green fluorescent proteins, and many of these scans have won awards in biological imaging competitions. An ideal introduction for students and advanced researchers alike, Illuminating Disease is an accessible yet deeply probing investigation into one of the most important developments in medical research of the last several decades.
New Models Of The Cell Nucleus Crowding Entropic Forces Phase Separation And Fractals
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Author : Ronald Hancock
language : en
Publisher: Academic Press
Release Date : 2013-12-27
New Models Of The Cell Nucleus Crowding Entropic Forces Phase Separation And Fractals written by Ronald Hancock and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-12-27 with Science categories.
International Review of Cell and Molecular Biology presents current advances and comprehensive reviews in cell biology--both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Impact factor for 2012: 4.973. Ideas from the fields of biophysics, physical chemistry, of polymer and colloid, and soft matter science have helped clarify the structure and functions of the cell nucleus. The development of powerful methods for modeling conformations and interactions of macromolecules has also contributed. The book aims to encourage cell and molecular biologists to become more familiar with and understand these new concepts and methods, and the crucial contributions they are making to our perception of the nucleus. This is the first volume to present a comprehensive review of New Models of the Cell Nucleus