Studies On Membrane Association And Targeting Of Rab Gtpases
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Studies On Membrane Association And Targeting Of Rab Gtpases
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Author : Anita Raquel Quintal Gomes
language : en
Publisher:
Release Date : 2002
Studies On Membrane Association And Targeting Of Rab Gtpases written by Anita Raquel Quintal Gomes and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with categories.
Biophysical Studies Of Rab Gtpase Membrane Binding
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Author :
language : en
Publisher:
Release Date : 2011
Biophysical Studies Of Rab Gtpase Membrane Binding written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
Gtpases Regulating Membrane Targeting And Fusion
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Author : W. E. Balch
language : en
Publisher: Gulf Professional Publishing
Release Date : 2005-12-13
Gtpases Regulating Membrane Targeting And Fusion written by W. E. Balch and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-12-13 with Science categories.
Rab GTPases now comprise a family of >63 members. They are emerging as the key hub element controlling the membrane architecture of eukaryotic cells. They are intimately involved in vesicle targeting and fusion in both the endocytic and exocytic pathways and direct the assembly and disassembly of protein complexes that include regulators (GEFs and GAPs), effectors (tethers/motors) and fusion components (SNAREs) that control membrane targeting and fusion. During the last 3 years the field has virtually exploded with the identification and characterization of many new Rab proteins and their effectors. Our understanding of how Rab GTPases control membrane function remains at its infancy. This volume of Methods in Enzymology, GTPases Regulating Membrane Targeting and Fusion, provides a wealth of new concepts, approaches and tools to study Rab proteins in the test tube and in living cells that will be of strong benefit to both established laboratories and new investigators in the field to elucidate Rab GTPase function in cellular development, differentiation and proliferation. Comprehensive overview of Rab GTPase phylogeny and systems biology Identification and characterization of Rab GEFs, GAPs and effectors General methodologies to study Rab GTPase function in vitro and in vivo using biochemical, molecular and microscopy approaches
Membrane Binding Properties Of Rab Gtpases
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Author : Guillaume Kulakowski
language : en
Publisher:
Release Date : 2017
Membrane Binding Properties Of Rab Gtpases written by Guillaume Kulakowski and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
RAB GTPases are major regulators of vesicular trafficking and localize to specific compartments. Deciphering the molecular mechanisms governing RAB localization is thus critical to understand intracellular transport processes. We have managed, for the first time, to incorporate purified and prenylated RABs into artificial membranes. By doing so, we observed that RAB6, but not RAB1 or RAB5, is able to promote by itself vesicle tethering. We believe that RAB6 is able to interact in trans with itself and to consequently drive homotypic membrane tethering. In the main part of this study, we investigated the physicochemical membrane requirements necessary for RAB recruitment. RAB1, RAB5 and RAB6 were all found to only localize to disordered membrane domains and to preferentially bind to curved membranes. We demonstrated that this specific recruitment of RAB1, RAB5 and RAB6 is primarily dependent on the hydrophobic insertion of their prenyl group into lipid packing defects. In contrast, RAB35 recruitment was primarily dependent on the presence of negatively charged lipids and was found to be modulated, to a lesser extent, by lipid packing defects. Although RAB4 and RAB11 were effectively recruited to purified Golgi fractions, in an effector-independent manner, membrane charges and lipid packing defects were not sufficient to promote their recruitment to synthetic vesicles; suggesting that RAB4 and RAB11 require more demanding membrane physicochemical properties. Our work demonstrates that the properties of membranes are critical for the regulation of RAB specific membrane targeting.
Mechanistic Studies Of Rab Gtpase Membrane Targeting And Cycling In Cells
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Author : Fu Li
language : en
Publisher:
Release Date : 2017
Mechanistic Studies Of Rab Gtpase Membrane Targeting And Cycling In Cells written by Fu Li and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.
Rab Gtpases And Membrane Trafficking
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Author : Guangpu Li
language : en
Publisher: Bentham Science Publishers
Release Date : 2012-05-21
Rab Gtpases And Membrane Trafficking written by Guangpu Li and has been published by Bentham Science Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-05-21 with Science categories.
"Ypt/Rab GTPases form the largest branch of the Ras-related small GTPase superfamily and regulate intracellular membrane trafficking in all eukaryotes. Since their discovery over two decades ago, a wealth of information has accumulated about the roles that"
Rab Gtpases
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Author : Guangpu Li (Molecular biologist)
language : en
Publisher:
Release Date : 2021
Rab Gtpases written by Guangpu Li (Molecular biologist) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with Guanosine triphosphatase categories.
This second edition volume expands on the previous edition with a discussion of new research and discoveries in the Rab field. Chapters in this book cover topics such as new information on Rab regulation and localization; interaction; function; and diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Rab GTPases: Methods and Protocols, Second Edition is a valuable resource for scientists working in the fields of Rab and other small GTPases, and beyond.
Regulators And Effectors Of Small Gtpases
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Author : William Edward Balch
language : en
Publisher: Academic Press
Release Date : 2006
Regulators And Effectors Of Small Gtpases written by William Edward Balch and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Science categories.
The Ras superfamily (>150 human members) encompasses Ras GTPases involved in cell proliferation, Rho GTPases involved in regulating the cytoskeleton, Rab GTPases involved in membrane targeting/fusion and a group of GTPases including Sar1, Arf, Arl and dynamin involved in vesicle budding/fission. These GTPases act as molecular switches and their activities are controlled by a large number of regulatory molecules that affect either GTP loading (guanine nucleotide exchange factors or GEFs) or GTP hydrolysis (GTPase activating proteins or GAPs). In their active state, they interact with a continually increasing, functionally complex array of downstream effectors. Since the last Methods in Enzymology volume on this topic in 2000, the study of Ras Family GTPases has witnessed a plethora of new directions and trends. With regards to the founding member of the Ras superfamily, the study of Ras in oncogenesis has seen the development and application of more advanced model cell culture and animal systems. The discovery of mutationally activated B-Raf in human cancers has injected renewed interest in this classical effector pathway of Ras. *Includes a database for Ras family proteins and their effectors and regulators *Complimentary to volume 406 coverage of the Rho family *Over 150 international contributors
Rab Gtpases
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Author : Guangpu Li (Molecular biologist)
language : en
Publisher:
Release Date : 2015
Rab Gtpases written by Guangpu Li (Molecular biologist) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with Biochemistry categories.
This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The book consists of 28 chapters, and starts with an overview of the Rab GTPase family. The next few chapters describe systematic approaches to the identification and classification of Rabs and Rab GAPs, as well as the detection of Rab isoprenylation and membrane distribution. The last few chapters examine the biochemical and functional properties of individual Rabs in the order of exocytic, recycling, and endocytic Rabs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Instructive and practical, Rab GTPases: Methods and Protocols approaches each topic with great detail and is a valuable resource for researchers and students interested in the field of Rab GTPases.
Encyclopedia Of Signaling Molecules
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Author :
language : en
Publisher:
Release Date : 19??
Encyclopedia Of Signaling Molecules written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 19?? with categories.