Sv40 Protocols
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Sv40 Protocols
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Author : Leda Raptis
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Sv40 Protocols written by Leda Raptis and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Simian virus 40 gained notoriety in the 1960s because it was found to be a contaminant of polio and adenovirus vaccines that had been administered to millions of healthy individuals worldwide. The public health implications of this revelation provided the initial impetus for an in-depth study of SV40 biology. Later work showed that SV40 DNA sequences as well as infectious virus are in fact found in human tumors and may have contributed to oncog- esis. It also turned out that SV40 uses mostly cellular machinery to carry out many steps in viral infection, which makes it a powerful probe for examining many fundamental questions in eukaryotic molecular biology. SV40 Pro- cols consolidates a number of well-tested step-by-step techniques in one v- ume; experts with hands-on experience in particular methods give detailed accounts of their optimized experimental protocols, so that the beginner, as well as more experienced researchers, may readily overcome problems of ambiguity often present in the literature. As with other DNA tumor viruses, the response of cultured cells to SV40 infection depends upon the species being infected. Monkey cells s- port virus production, which leads to their death, whereas rodent cells p- duce only the early proteins and acquire a transformed phenotype. Thus, SV40 Protocols is organized in two sections. The first relates to assays of the lytic cycle of the virus, and the second deals with transformation.
The Sv 40 Virus
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Author : United States. Congress. House. Committee on Government Reform. Subcommittee on Human Rights and Wellness
language : en
Publisher:
Release Date : 2004
The Sv 40 Virus written by United States. Congress. House. Committee on Government Reform. Subcommittee on Human Rights and Wellness and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Medical categories.
Adhesion Protein Protocols
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Author : Elisabetta Dejana
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Adhesion Protein Protocols written by Elisabetta Dejana and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Adhesion molecules are of fundamental importance in the regulation of immunity, inflammation, tissue remodeling, and embryonic development. They comprise different families of homologous proteins, such as selectins, integrins, cadherins, and immunoglobins. In addition, beyond these groups, other str- tures with adhesive properties, such as proteoglycans, occludin, and CD44, have been characterized recently. An understanding of the type and characteristics of adhesive molecules expressed by the different cell types and the possibility of manipulating their activity promises considerable clinical potential. Antibodies, small peptidic and nonpeptidic molecules, have recently been used to inhibit thrombosis by blocking platelet aggregation or inflammation through inhibition of leukocyte infiltration and adhesion. Inhibitors of adhesive molecules are used in expe- mental systems for the study of tumor growth and dissemination. Among major goals in the field are the identification of new members of the known adhesive protein families and of independent new adhesive structures. After structural characterization, even more demanding is the study of the biological activity of the new proteins, and the development of simple, rapid tests for the screening of possible inhibitors. In this regard, the production of such reagents as fragments and antibodies would help define the structure–function relati- ship of individual proteins. Data available in the literature show the complexity of the adhesive process and how different molecular epitopes might contribute to the adhesive properties of a single structure. Finally, a new area of investi- tion is the characterization of the intracellular signaling cascade triggered by the engagement of transmembrane adhesive proteins.
Chromatin Protocols
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Author : Peter B. Becker
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Chromatin Protocols written by Peter B. Becker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
More than 40 years after the discovery of the nucleosome as the fun- mental unit of chromatin, the multifaceted problem of how variations in ch- matin structure affect the activity of the eukaryotic genome has not been solved. However, during the past few years research on chromatin structure and fu- tion has gained considerable momentum, and impressive progress has been made at the level of concept development as well as filling in crucial detail. The structure of the nucleosome has been visualized at unprecedented reso- tion. Powerful multisubunit enzymes have been identified that alter histone/ DNA interactions in ways that expose regulatory sequences to factors initi- ing and regulating such nuclear processes as transcription. Though the imp- tance of posttranslational modifications of histones, notably their acetylation, has long been known, the finding that a number of bona fide regulators increase transcription by acetylating nucleosomes has lent new support to the old idea that the process of gene regulation is intimately related to the nature of the chromatin environment. A wealth of nonhistone proteins contribute to a continuum of structures with distinct biochemical properties and varying degrees of DNA condensation. Perhaps the most important conclusion from a large number of studies is a fresh appreciation of the dynamic nature of chromatin structure, the built-in flexibility providing the basis for regulation.
Concepts In Genetic Medicine
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Author : Boro Dropulic
language : en
Publisher: John Wiley & Sons
Release Date : 2008-01-03
Concepts In Genetic Medicine written by Boro Dropulic and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-01-03 with Medical categories.
This book delivers a collection of organized and succinct reviews in the field of therapeutic genetic medicine presented by a carefully selected group of top experts. Each chapter focuses on a single, current topic and explains issues in the development of genetic treatments, critical challenges and strategies for implementing them, and future directions in research and translational applications. The book is complemented with uniformly designed illustrations and a supplementary Web site with a PowerPoint presentation for educators.
Protein Structure Stability And Folding
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Author : Kenneth P. Murphy
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Protein Structure Stability And Folding written by Kenneth P. Murphy and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
In Protein Structure, Stability, and Folding, Kenneth P. Murphy and a panel of internationally recognized investigators describe some of the newest experimental and theoretical methods for investigating these critical events and processes. Among the techniques discussed are the many methods for calculating many of protein stability and dynamics from knowledge of the structure, and for performing molecular dynamics simulations of protein unfolding. New experimental approaches presented include the use of co-solvents, novel applications of hydrogen exchange techniques, temperature-jump methods for looking at folding events, and new strategies for mutagenesis experiments. Unique in its powerful combination of theory and practice, Protein Structure, Stability, and Folding offers protein and biophysical chemists the means to gain a more comprehensive understanding of some of this complex area by detailing many of the major techniques in use today.
Gtpase Protocols
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Author : Ed Manser
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Gtpase Protocols written by Ed Manser and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
In the last 10 years researchers have firmly established key roles for R- related GTPases in almost every aspect of cell biology. In the 1980s the pro- oncogene Ras itself was the focus of interest, though in the 1990s this shifted to the increasing variety of Ras-related proteins. In this new decade much yet needs to be done to establish the role for all the small GTPases now uncovered by the human genome project. In particular, these GTPases need to be und- stood in the appropriate biochemical and cellular contexts. In the process of trying to uncover the role of these versatile proteins, a variety of novel te- niques and methodologies has been developed. These now enable investi- tors to move easily within a diversity of fields ranging from structural studies to real-time in vivo analysis of a GTPase. In recognition of the need for access to key background methodologies, GTPase Protocols: The Ras Superfamily is devoted to techniques that are pr- ently widely used and that will continue to be the standard for researchers worldwide. Each chapter is aimed at supplying detailed methodologies to allow reproduction in any laboratory, while also providing the general pr- ciples on which the methods are based. Some of the techniques grouped in the first section apply broadly to small GTPases, whereas others in Part II are more applicable within each GTPase subfamily.
Biostatistical Methods
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Author : Stephen W. Looney
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Biostatistical Methods written by Stephen W. Looney and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Leading biostatisticians and biomedical researchers describe many of the key techniques used to solve commonly occurring data analytic problems in molecular biology, and demonstrate how these methods can be used in the development of new markers for exposure to a risk factor or for disease outcomes. Major areas of application include microarray analysis, proteomic studies, image quantitation, genetic susceptibility and association, evaluation of new biomarkers, and power analysis and sample size.
Gene Isolation And Mapping Protocols
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Author : Jacqueline Boultwood
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Gene Isolation And Mapping Protocols written by Jacqueline Boultwood and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
An unprecedented collection of all the most up-to-date techniques for gene isolation and mapping, including the latest methods for gene characterization using database analyses. This collection of thoroughly tested recipes also includes chapters for the computational analysis of novel cDNA sequences with up-to-the-minute information on basic sequence analysis, sequence similarity searches, exon detection and similarity searches, and the prediction of gene function. Its state-of-the-art methods constitute indispensable tools for all scientists engaged in the search for specific disease genes, or in the general advancement of the human genome project.
Protocols In Human Molecular Genetics
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Author : Christopher G. Mathew
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Protocols In Human Molecular Genetics written by Christopher G. Mathew and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Medical categories.
Extraordinary advances have been made in the field of human molecular genetics during the past five years. The ability to amplify a specific region ofDNA a millionfold in a few hours using the polymerase chain reaction has led to the rapid identification of mutations in human disease and of DNA sequence polymorphisms on every human chro- some. DNA fragments of up to 1 megabase in length can now be resolved by pulsed-field gel electrophoresis to create long-range physical maps of important regions of the genome, and can be cloned in the form of yeast artificial chromosomes. The discovery of highly variable "minisatellite" DNA sequences has led to the development of DNA fingerprinting. The application of these techniques to the study of the human genome has culminated in major advances such as the cloning of the cystic fibrosis gene, the construction of genetic linkage maps of each human chro- some, the mapping of many genes responsible for human inherited d- orders, genetic fingerprinting of forensic specimens, and the detection of mutations involved in the development of human tumors. Although many of the new techniques in molecular genetics can be learned relatively easily, it is often difficult for a researcher to obtain all of the relevant information necessary for getting up a technique and applying it successfully. The information available in the research lite- ture often lacks the depth of description that the new user requires.