Techniques In Somatic Cell Genetics

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Techniques In Somatic Cell Genetics

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Author : Jerry W. Shay
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-21

Techniques In Somatic Cell Genetics written by Jerry W. Shay and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-21 with Medical categories.

Somatic cell genetics is an exciting and rapidly expanding field of research. Since descriptions of the major experimental techniques in the field are scattered throughout various journals and other publications, there is a real need for a single reference source for both established investigators and students in the field. In addition, technical reports are frequently abridged such that many researchers are discouraged from attempting to adopt the appropriate methodology. This book, therefore, describes in detail the many recent technical advances in such areas of somatic cell genetics as transfer mediated by liposomes, erythrocyte ghosts, chromosomes, micro cells, mito chondria, and isolated nuclear DNA. These techniques have increased our understanding of the organization and regulation of eukaryotic cells. The production of antibiotic-resistant cell lines and their use in studying cytoplasmic inheritance are also included. Evidence for the cytoplasmic regulation of nuclear gene expression in eukaryotic cells is rapidly accumu lating following the characterization of cytoplasmic mutations. The produc tion of nuclear-coded mutations, their use in standard cell hybridization, and recent advances in techniques for fusing whole cells or cell components are also described.

New Techniques In Somatic Cell Genetics

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Author : Alan Garth Tunnacliffe
language : en
Publisher:
Release Date : 1984

New Techniques In Somatic Cell Genetics written by Alan Garth Tunnacliffe and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with categories.

Plant Improvement And Somatic Cell Genetics

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Author : Indra Asil
language : en
Publisher: Elsevier
Release Date : 2012-12-02

Plant Improvement And Somatic Cell Genetics written by Indra Asil and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.

Plant Improvement and Somatic Cell Genetics includes all but one of the papers presented at two symposia held during the XIII International Botanical Congress in Sydney, Australia, on August 21-28, 1981. ""Frontiers in Plant Breeding"" and ""Cell Culture and Somatic Cell Genetics in Plant Biology"" highlight the ways in which plant breeding techniques can improve crops. The book explores the potentials as well as the limitations of plant breeding, and cellular and molecular techniques in plant improvement. Comprised of 14 chapters, this volume begins with an overview of the potential applications of exotic germplasm for tomato and cereal crop improvement. It continues with a discussion of multiline breeding, breeding of crop plants that can tolerate soil stresses, combining genomes by means of conventional methods, use of embryo culture in interspecific hybridization, use of haploids in plant improvement, and somaclonal variation and somatic hybridization as new techniques for plant improvement. The reader is also introduced to plant cell culture, as well as somatic cell genetics of cereals and grasses, somatic cell fusion for inducing cytoplasmic exchange, uses of cell culture mutants, genetic transformation of plant cells by experimental procedures in the context of plant genetic engineering, and use of molecular biology techniques for recognition and modification of crop plant genotypes. This book will be a useful resource for scientists and plant breeders interested in applying somatic cell genetics for crop improvement.

The Cells Of The Body

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Author : Henry Harris
language : en
Publisher: CSHL Press
Release Date : 1995

The Cells Of The Body written by Henry Harris and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with Science categories.

This book is the first scholarly history of research into the genetics of body cells, from its origins in the 19th century to the present day. Henry Harris, a well-known writer and a distinguished investigator in cell biology and cancer genetics, brings an unusually informed perspective to the technical aspects of his subject. He has written a book to be enjoyed not just by professional historians of science, but by working scientists in genetics, cell biology, and cancer research, from the graduate student level upwards. Its readers will derive a richer understanding of how and why the cells of the body are studied in the way that they are today.

Development And Application Of A General Method For Somatic Cell Genetics

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Author : Tim Wang (Ph. D.)
language : en
Publisher:
Release Date : 2017

Development And Application Of A General Method For Somatic Cell Genetics written by Tim Wang (Ph. D.) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with categories.

Genetic analysis, or genetic screening, is an efficient method for identifying the set of genes involved in a biological process. In microorganisms, powerful techniques enable systematic loss-of-function screens, which have yielded critical insights into the molecular basis of many fundamental cellular pathways. In human cells, however, prevailing screening methods fall short - leaving our understanding of human genes incomplete. Here, I describe the development of a general method for genetic screening in mammalian cells using the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system. In this approach, libraries of knockout mutants can be generated in any cultured cell line using large-scale lentiviral single-guide RNA (sgRNA) pools and screened under both positive and negative selection. After the initial, proof-of-principle experiments, I constructed a genome-wide sgRNA library and screened for genes required for the proliferation and survival of the nearhaploid chronic myeloid leukemia cell line, KBM7. The unusual karyotype of these cells also allowed an independent screening approach, involving gene-trap insertional mutagenesis, to find cell-essential genes. Together, these screens converged on a highly overlapping gene set that was enriched for genes that encode components of fundamental pathways, are expressed at high levels, and contain few inactivating polymorphisms in the human population. To broadly survey patterns of human gene essentiality, I conducted CRISPR-based screens across many more cell lines and cancer types. Overall, these studies revealed a large set of common essential genes involved in housekeeping processes but, interestingly, also pinpointed differences specific to each cell line that reflected its developmental origin, oncogenic drivers, paralogous gene expression pattern, and chromosomal structure. By mapping sets of genes displaying variable but correlated essentiality across lines, I uncovered functionally interacting gene networks. Using this dataset, I also identified genetic vulnerabilities associated with oncogenic Ras which may be exploited for anti-cancer therapy. Last, in collaboration with Bruce Walker and Nir Hacohen's labs, I have conducted a screen for host factors (i.e. human genes) that are required to support HIV infection in a physiologically relevant cell line model. Collectively, these results establish CRISPR-based screens as a powerful tool for systematic genetic analysis in mammalian cells.

Hybridization Of Somatic Cells

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Author : Boris Ephrussi
language : en
Publisher: Princeton University Press
Release Date : 2015-03-08

Hybridization Of Somatic Cells written by Boris Ephrussi and has been published by Princeton University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-03-08 with Science categories.

Since its discovery in I960, the hybridization of somatic cells has evolved from a biological curiosity into an analytical method that today underlies nearly all investigations of the genetic aspects of various biological phenomena. As an eyewitness to this development from its inception forward, Boris Ephrussi here relates the history of somatic hybridization and the formation of its methodology, lie follows with a discussion of the characteristics and properties of the resultant hybrid cells. Together, these topics comprise an authoritative introduction to the principles of the technique. Dr. Ephrussi proceeds to an examination in greater detail of three specific areas of biological research to which the techniques of hybridization are currently being applied with promising consequences. Thus the major part of the book deals with applications of somatic hybridization to mammalian genetics, cell differentiation, and cancer. Originally published in 1972. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Cell Culture And Somatic Cell Genetics Of Plants

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Author : I. K. Vasil
language : en
Publisher:
Release Date : 1984

Cell Culture And Somatic Cell Genetics Of Plants written by I. K. Vasil and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Cell culture categories.

V. 1. Laboratory procedures and their applications.--v. 2. Cell growth, nutrition, cytodifferentiation, and cryopreservation.--v. 3. Plant regeneration and genetic variability.--v. 4. Cell culture in phytochemistry.--v. 5. Phytoche micals in plant cell cultures.--v. 6. Molecular biology of plant nuclear genes. --v. 7A. The molecular biology of plastids.--v. 7B. The photosynthetic apparatus: molecular biology and operation.--v. 8. Scale-up and automation in plant propagation.

Somatic Cell Hybrids

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Author : Abbott
language : en
Publisher: Oxford University Press, USA
Release Date : 1995-03

Somatic Cell Hybrids written by Abbott and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-03 with Science categories.

Describing the background to a technique widely used in human genetics, this study analyzes the uses of a particular type of cell culture. These uses include the mapping of human genes, a key aspect of the human genome project.

The Investigation Of Tumour Cell Products Using The Techniques Of Somatic Cell Genetics

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Author : Denise Sheer
language : en
Publisher:
Release Date : 1980

The Investigation Of Tumour Cell Products Using The Techniques Of Somatic Cell Genetics written by Denise Sheer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1980 with Carcinogenesis categories.

Somatic Cell Genetics Of Woody Plants

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Author : M.R. Ahuja
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Somatic Cell Genetics Of Woody Plants written by M.R. Ahuja and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Technology & Engineering categories.

Most forest tree species were considered recalcitrant a decade ago, but now with the improved in vitro techniques some progress has been made towards culture-of tree species. Micro propagation has been achieved from the juvenile tissues of a number of forest tree species. On the other hand, tissues from most mature trees are still very difficult to grow and differen tiate in vitro. Nevertheless, there has been slow but steady progress in the application of tissue culture technology for culture of tissues, organs, cells and protoplasts of tree species. As compared to most agricultural crops, and herbaceous plant species, trees are a different lot. They have long gene ration cycles. They are highly heterozygous and have a large reservoir of genetic variability. Because of this genetic variability, their response in vitro is also variable. On a single medium, the response of tissues from different trees (genotypes) of a single species may be quite different: some responding by induction of growth and differentiation, while others showing minimal or no growth at all. That makes the somatic cell genetics of woody plants somewhat difficult, but at the same time interesting.