The Development Of Reporter Genes For In Vivo Imaging
DOWNLOAD
Download The Development Of Reporter Genes For In Vivo Imaging PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get The Development Of Reporter Genes For In Vivo Imaging book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
The Development Of Reporter Genes For In Vivo Imaging
DOWNLOAD
Author : Peter Stephen Patrick
language : en
Publisher:
Release Date : 2014
The Development Of Reporter Genes For In Vivo Imaging written by Peter Stephen Patrick and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.
Molecular Imaging With Reporter Genes
DOWNLOAD
Author : Sanjiv Sam Gambhir
language : en
Publisher: Cambridge University Press
Release Date : 2010-05-31
Molecular Imaging With Reporter Genes written by Sanjiv Sam Gambhir and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-05-31 with Science categories.
Reporter genes have been used for several decades to study regulation of gene expression in vivo. However, it was little more than a decade ago that a new class of reporter genes was developed for imaging molecular events within living subjects. By following the interactions of protein molecules, researchers can resolve the complex chemical pathways that living cells utilise. This book focuses on this group of imaging reporter genes, starting with detailed descriptions of all reporter genes from different imaging modalities, including optical, MRI, and radionuclide-based imaging. Key scientists in the field explain how to enhance reporter gene imaging utility through instrumentation and the various applications of this technology. This is the first comprehensive book on all aspects of reporter gene imaging, detailing what is known in the field and future goals for research. Investigators in biomedical sciences, physicians, and the biotechnology and pharmaceutical industries will benefit from topics covered.
Development Of Reporter Genes For In Vivo Imaging Of Staphylococcus Aureus
DOWNLOAD
Author : Rochelle Marie Lamb
language : en
Publisher:
Release Date : 2010
Development Of Reporter Genes For In Vivo Imaging Of Staphylococcus Aureus written by Rochelle Marie Lamb and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Molecular probes categories.
Reporter Genes
DOWNLOAD
Author : Donald S. Anson
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-09-13
Reporter Genes written by Donald S. Anson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-09-13 with Science categories.
Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of ß-galactosidase in embryos and tissues, immunohistochemical detection of ß-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists.
Reporter Gene Assays
DOWNLOAD
Author : Robert Damoiseaux
language : en
Publisher:
Release Date : 2018
Reporter Gene Assays written by Robert Damoiseaux and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Reporter genes categories.
Textbook Of In Vivo Imaging In Vertebrates
DOWNLOAD
Author : Vasilis Ntziachristos
language : en
Publisher: John Wiley & Sons
Release Date : 2007-08-20
Textbook Of In Vivo Imaging In Vertebrates written by Vasilis Ntziachristos and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-20 with Science categories.
This book describes the new imaging techniques being developed to monitor physiological, cellular and subcellular function within living animals. This exciting field of imaging science brings together physics, chemistry, engineering, biology and medicine to yield powerful and versatile imaging approaches. By combining advanced non-invasive imaging technologies with new mechanisms for visualizing biochemical events and protein and gene function, non-invasive vertebrate imaging enables the in vivo study of biology and offers rapid routes from basic discovery to drug development and clinical application. Combined with the availability of an increasing number of animal models of human disease, and the ability to perform longitudinal studies of disease evolution and of the long-term effects of therapeutic procedures, this new technology offers the next generation of tools for biomedical research. Well illustrated, largely in colour, the book reviews the most common and technologically advanced methods for vertebrate imaging, presented in a clear, comprehensive format. The basic principles are described, followed by several examples of the use of imaging in the study of living multicellular organisms, concentrating on small animal models of human diseases. The book illustrates: The types of information that can be obtained with modern in vivo imaging; The substitution of imaging methods for more destructive histological techniques; The advantages conferred by in vivo imaging in building a more accurate picture of the response of tissues to stimuli over time while significantly reducing the number of animals required for such studies. Part 1 describes current techniques in in vivo imaging, providing specialists and laboratory scientists from all disciplines with clear and helpful information regarding the tools available for their specific research field. Part 2 looks in more detail at imaging organ development and function, covering the brain, heart, lung and others. Part 3 describes the use of imaging to monitor various new types of therapy, following the reaction in an individual organism over time, e.g. after gene or cell therapy. Most chapters are written by teams of physicists and biologists, giving a balanced coherent description of each technique and its potential applications.
In Vivo Cellular And Molecular Imaging
DOWNLOAD
Author : Eric T. Ahrens
language : en
Publisher: Gulf Professional Publishing
Release Date : 2005-11-09
In Vivo Cellular And Molecular Imaging written by Eric T. Ahrens and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-11-09 with Medical categories.
In recent times there has been an explosive expansion of new imaging methodologies that are capable of visualizing specific populations of cells and molecular events in vivo. Vital imaging enhances our ability to study animal models of human development and disease, such as cancers, cardiovascular disease, diabetes, and Alzheimer's. Furthermore, non-invasive imaging may ultimately be useful for monitoring new generations of clinical molecular and cellular therapeutics, such as those utilizing viral vectors and stem cells. These new capabilities have been facilitated by the development of new imaging probes or reagents that target specific cell types, are chemically responsive to physiology, or are responsive to the presence of specific molecules, such as nucleic acids or enzymes. This volume provides an introduction to some of the most exciting methods and applications of emerging non-invasive imaging technologies using magnetic resonance imaging (MRI), positron emission tomography (PET), and various biophotonic approaches. Highlighted, are recent developments in reagent design that impart unique abilities to these imaging modalities to elucidate biological processes in vivo. * Includes 9 chapters by expert researchers in the field of imaging * Introduces new methods and applications of non-invasive imaging technologies * Covers ermerging topics in imaging such as in vivo cell cancer cells, imaging of autoimmune diseases, and maganetic resonance imaging
Molecular Imaging Basic Principles And Applications In Biomedical Research
DOWNLOAD
Author : Markus Rudin
language : en
Publisher: World Scientific
Release Date : 2005-09-29
Molecular Imaging Basic Principles And Applications In Biomedical Research written by Markus Rudin and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-09-29 with Science categories.
Molecular imaging is a rapidly emerging field that translates many concepts developed for molecular biology and cellular imaging to the in vivo imaging of intact organisms. The technique allows the study of molecular biological events in their full context and will therefore become an indispensable tool for biomedical research and drug discovery and development.This volume familiarizes the reader with the concepts of imaging and molecular imaging in particular. Basic principles of imaging technologies, reporter moieties for the various imaging modalities and the design of target reporter constructs are described in the first part. The second part illustrates how these tools can be used to visualize relevant molecular events: the biodistribution of drugs/ligands, the expression of drug targets (receptors, enzymes), and the consequences of the molecular drug-target interactions (pathway activations, system responses). A final chapter deals with visualization of cell migration (cell therapies).
Molecular Imaging With Reporter Genes
DOWNLOAD
Author : Sanjiv Sam Gambhir
language : en
Publisher:
Release Date : 2014-05-14
Molecular Imaging With Reporter Genes written by Sanjiv Sam Gambhir and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-14 with Medical categories.
Focuses on the imaging of reporter genes in living subjects, a technology that was developed only ten years ago.
Development Of Molecular And Cellular Imaging Tools To Evaluate Gene And Cell Based Therapeutic Strategies In Vivo
DOWNLOAD
Author : Jixiang Xia
language : en
Publisher:
Release Date : 2011
Development Of Molecular And Cellular Imaging Tools To Evaluate Gene And Cell Based Therapeutic Strategies In Vivo written by Jixiang Xia and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Bioluminescence categories.
Molecular imaging modalities are important tools to evaluate the efficacy of gene delivery systems and cell-based therapies. Development and application of these modalities will advance our understanding of the mechanism of transgene expression and cell fate and functions. Physical gene transfer methods hold many advantages over viral vectors among gene therapeutic strategies. Here, we evaluated the efficacy of biolistic ("gene gun") gene targeting to tissues with non-invasive bioluminescence imaging (BLI) methods. Plasmids carrying the firefly luciferase reporter gene were transfected into mouse skin and liver using biolistics, and BLI was measured at various time points after transfer. With optimized DNA loading ratio (DLRs), reporter gene expression reached to peak 1day after transfer to mouse skin, and the maximum depth of tissue penetration was between 200-300[micrometer]. Similar peak expression of reporter gene was found in mouse liver but the expression was relatively stable 4-8 days post-biolistic gene transfer and remained for up to two weeks afterward. Our results demonstrated BLI was an efficient strategy for evaluation of reporter gene expression in the same animals over a period of up to two weeks in vivo. Different tissues showed different expression kinetics, suggesting that this is an important parameter to consider when developing gene therapy strategies for different target tissues. We also employed BLI to measure differentiation of mouse embryonic stem (ES) cells into beating cardiomyocytes in vitro and in vivo. A subset of these cardiomyocytes appears to be derived from an adrenergic lineage that ultimately contribute to substantial numbers of cardiomyocytes primarily on the left side of the heart. At present, it is unclear what the precise role of these cardiac adrenergic cells is with respect to heart development, though it is known that adrenergic hormones (adrenaline and noradrenaline) are essential for embryonic development since mice lacking them die from apparent heart failure during the prenatal period. To identify and characterize cardiac adrenergic cells, we developed a novel mouse genetic model in which the nuclear-localized enhanced green fluorescent protein (nEGFP) reporter gene was targeted to the first exon of the Phenylethanoamine N-transferase (Pnmt) gene, which encodes for the enzyme that converts noradrenaline to adrenaline, and hence serves as a marker for adrenergic cells. Our results demonstrate this knock-in strategy effectively marked adrenergic cells in both fetal and adult mice. Expression of nEGFP was found in Pnmt-positive cells of the adult adrenal medulla, as expected. Pnmt-nEGFP expression also recapitulated endogenous Pnmt expression in the embryonic mouse heart. In addition, nEGFP and Pnmt expression were induced in parallel during differentiation of pluripotent mouse ES cells into beating cardiomyocytes. This new mouse genetic model provides a useful new tool for studying the properties of adrenergic cells in different tissues. We also identified two limitations of the Pnmt-nEGFP model. One is that the amount of nEGFP expressed within individual adrenergic cells was highly variable. Secondly, expression of nEGFP in the embryonic heart was of low abundance and difficult to distinguish from background autofluorescence. To overcome these limitations, we developed two alternative genetic models to investigate adrenergic cells: (1) Mouse embryonic stem cells, which have been previously targeted with Pnmt-Cre recombinase gene, were additionally targeted with a dual reporter plasmid which covered both a loxP-flanked cDNA of red fluorescence protein (HcRed) and also EGFP. Under the undifferentiated status, cells emit red fluorescence as transcription stops before EGFP coding sequence. After differentiation into beating cardiomyoctyes, some cells switch fluorescence from red to green, indicating that excision of loxP-flanked sequences by Cre since Pnmt had been activated. (2) A surface marker, truncated low-affinity nerve growth factor receptor ([delta]LNGFR) was used as the reporter gene as cells expressing this marker can be enriched by magnetic-activated cell sorting (MACS), a potentially efficient way to yield highly purified positive cells at low input abundance in a population. Through a series of subcloning steps, the targeting construct, Pnmt-[delta]LNGFR-Neo-DTA was created and electroporated into 7AC5EYFP embryonic stem cells. Correctly targeted cells were selected by positive and negative screening. These cells provide a new tool with which to identify, isolate, and characterize the function of adrenergic cells in the developing heart, adrenal gland, and other tissues where adrenergic cells make important contributions.