Molecular Imaging With Reporter Genes
DOWNLOAD
Download Molecular Imaging With Reporter Genes PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Molecular Imaging With Reporter Genes book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Molecular Imaging With Reporter Genes
DOWNLOAD
Author : Sanjiv Sam Gambhir
language : en
Publisher: Cambridge University Press
Release Date : 2010-05-31
Molecular Imaging With Reporter Genes written by Sanjiv Sam Gambhir and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-05-31 with Science categories.
Reporter genes have been used for several decades to study regulation of gene expression in vivo. However, it was little more than a decade ago that a new class of reporter genes was developed for imaging molecular events within living subjects. By following the interactions of protein molecules, researchers can resolve the complex chemical pathways that living cells utilise. This book focuses on this group of imaging reporter genes, starting with detailed descriptions of all reporter genes from different imaging modalities, including optical, MRI, and radionuclide-based imaging. Key scientists in the field explain how to enhance reporter gene imaging utility through instrumentation and the various applications of this technology. This is the first comprehensive book on all aspects of reporter gene imaging, detailing what is known in the field and future goals for research. Investigators in biomedical sciences, physicians, and the biotechnology and pharmaceutical industries will benefit from topics covered.
Molecular Imaging With Reporter Genes
DOWNLOAD
Author : Sanjiv Sam Gambhir
language : en
Publisher:
Release Date : 2014-05-14
Molecular Imaging With Reporter Genes written by Sanjiv Sam Gambhir and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-14 with Medical categories.
Focuses on the imaging of reporter genes in living subjects, a technology that was developed only ten years ago.
Reporter Gene Imaging
DOWNLOAD
Author : Purnima Dubey
language : en
Publisher:
Release Date : 2018
Reporter Gene Imaging written by Purnima Dubey and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Electronic books categories.
This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.
Reporter Genes
DOWNLOAD
Author : Donald S. Anson
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-09-13
Reporter Genes written by Donald S. Anson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-09-13 with Science categories.
Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of ß-galactosidase in embryos and tissues, immunohistochemical detection of ß-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists.
Reporter Gene Assays
DOWNLOAD
Author : Robert Damoiseaux
language : en
Publisher: Humana Press
Release Date : 2018-04-19
Reporter Gene Assays written by Robert Damoiseaux and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-19 with Medical categories.
This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs. Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interested in learning more about reporter genes.
Molecular Imaging Of Gene Expression At The Level Of Rna In Living Animals
DOWNLOAD
Author : Zachary Floyd Walls
language : en
Publisher:
Release Date : 2008
Molecular Imaging Of Gene Expression At The Level Of Rna In Living Animals written by Zachary Floyd Walls and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with categories.
Reporter Genes
DOWNLOAD
Author : Don Anson
language : en
Publisher: Humana Press
Release Date : 2007-09-13
Reporter Genes written by Don Anson and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-09-13 with Science categories.
Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. This book describes practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available.
Transcription Based Molecular Imaging And Gene Therapy For Castration Resistant And Metastatic Prostate Cancer In Translational Models
DOWNLOAD
Author : Ziyue Jiang
language : en
Publisher:
Release Date : 2013
Transcription Based Molecular Imaging And Gene Therapy For Castration Resistant And Metastatic Prostate Cancer In Translational Models written by Ziyue Jiang and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.
The advanced stage of prostate cancer is the second leading cause of cancer-related death for American men. Novel, effective treatment options and more cancer-specific diagnostic tools are urgently needed to facilitate patient management. Here, we explored the construction and application of an array of gene-based molecular imaging and therapeutic vectors in a variety of clinically relevant settings. These vectors exploit prostate cancer-specific promoters to control the transcription of imaging reporter genes or therapeutic genes, and thus can achieve stringent cancer selectivity. We showed that gene-based imaging vectors can detect prostate cancer metastasis earlier than conventional imaging modalities; we also developed a dual-reporter imaging system that can specifically interrogate the activation/inhibition status of androgen receptor, and thus monitor the efficacy of androgen deprivation and androgen receptor blockade therapies. Further, we explored the applicability of gene-based vectors in detecting circulating tumor cells and have obtained promising results. Moreover, we employed two strategies to improve the clinical usability of viral vectors in immunocompetent hosts (such as humans). In the first strategy, we used a broad-acting immunosuppressant, rapamycin, to reduce the scale of adenovirus-induced inflammatory responses and also to decrease the eliminating effects of host adaptive immune system on viral vectors and viral-infected, transgene-expressing cells. Rapamycin significantly augmented the magnitude and prolonged the duration of virus-delivered transgene expression, and thus enhanced the diagnostic capability of these imaging vectors. The second approach took advantage of the versatile surface modifying ability of non-viral reagents. We designed a polypeptide coating that can non-covalently bind to adenoviral particles and confer protections against blood components such as neutralizing antibodies and coagulation factors. The coating significantly decreased the inhibitory effects of neutralizing antibodies on adenoviral transduction and markedly abrogated coagulation factors-mediated hepatocyte infection. Furthermore, this polypeptide coating reduced the antigenicity of adenoviral vectors in vivo, greatly diminished anti-adenovirus antibody production, and therefore could facilitate repeated administration of viral vectors in immunocompetent hosts. Overall, we herein present a substantial amount of evidence supporting the pre-clinical and translational use of gene-based imaging and therapeutic vectors to assist the management of patients with advanced, treatment-refractory prostate cancer.
Imaging Reporter Transgene Expression In Living Subjects Using Positron Emission Tomography
DOWNLOAD
Author : Shahriar Yaghoubi
language : en
Publisher:
Release Date : 2002
Imaging Reporter Transgene Expression In Living Subjects Using Positron Emission Tomography written by Shahriar Yaghoubi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with categories.
Molecular Imaging With Genetically Programmed Nanoparticles
DOWNLOAD
Author : Donna Goldhawk Goldhawk
language : en
Publisher:
Release Date : 2022
Molecular Imaging With Genetically Programmed Nanoparticles written by Donna Goldhawk Goldhawk and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with Electronic books categories.
Nanoparticle research has greatly benefitted medical imaging platforms by generating new signals, enhancing detection sensitivity, and expanding both clinical and preclinical applications. For magnetic resonance imaging, the fabrication of superparamagnetic iron oxide nanoparticles has provided a means of detecting cells and has paved the way for magnetic particle imaging. As the field of molecular imaging grows and enables the tracking of cells and their molecular activities so does the possibility of tracking genetically programmed biomarkers. This chapter discusses the advantages and challenges of gene-based contrast, using the bacterial magnetosome model to highlight the requirements of in vivo iron biomineralization and reporter gene expression for magnetic resonance signal detection. New information about magnetosome protein interactions in non-magnetic mammalian cells is considered in the light of design and application(s) of a rudimentary magnetosome-like nanoparticle for molecular imaging. Central to this is the hypothesis that a magnetosome root structure is defined by essential magnetosome genes, whose expression positions the biomineral in a given membrane compartment, in any cell type. The use of synthetic biology for programming multi-component structures not only broadens the scope of reporter gene expression for molecular MRI but also facilitates the tracking of cell therapies.