Reporter Gene Imaging
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Molecular Imaging With Reporter Genes
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Author : Sanjiv Sam Gambhir
language : en
Publisher: Cambridge University Press
Release Date : 2010-05-31
Molecular Imaging With Reporter Genes written by Sanjiv Sam Gambhir and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-05-31 with Science categories.
Reporter genes have been used for several decades to study regulation of gene expression in vivo. However, it was little more than a decade ago that a new class of reporter genes was developed for imaging molecular events within living subjects. By following the interactions of protein molecules, researchers can resolve the complex chemical pathways that living cells utilise. This book focuses on this group of imaging reporter genes, starting with detailed descriptions of all reporter genes from different imaging modalities, including optical, MRI, and radionuclide-based imaging. Key scientists in the field explain how to enhance reporter gene imaging utility through instrumentation and the various applications of this technology. This is the first comprehensive book on all aspects of reporter gene imaging, detailing what is known in the field and future goals for research. Investigators in biomedical sciences, physicians, and the biotechnology and pharmaceutical industries will benefit from topics covered.
Reporter Gene Imaging
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Author : Purnima Dubey
language : en
Publisher: Humana
Release Date : 2019-06-16
Reporter Gene Imaging written by Purnima Dubey and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-16 with Medical categories.
This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.
Molecular Imaging With Reporter Genes
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Author : Sanjiv Sam Gambhir
language : en
Publisher:
Release Date : 2014-05-14
Molecular Imaging With Reporter Genes written by Sanjiv Sam Gambhir and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-05-14 with Medical categories.
Focuses on the imaging of reporter genes in living subjects, a technology that was developed only ten years ago.
Reporter Genes
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Author : Donald S. Anson
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-09-13
Reporter Genes written by Donald S. Anson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-09-13 with Science categories.
Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. In Reporter Genes: A Practical Guide, renowned researchers describe practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available, concentrating on those marker genes that are currently most commonly used. Among the topics covered in this volume are methodologies for staining and visualization of ß-galactosidase in embryos and tissues, immunohistochemical detection of ß-galactosidase, detection of reporter gene expression in murine airways, and three dimensional analysis of molecular signals with episcopic imaging techniques. Also covered in this groundbreaking text are fluorescent proteins, detection of GFP during nervous system development, fluorescent protein reporter systems for single cell measurements, and non-invasive imaging of molecular events. Comprehensive and illuminating, Reporter Genes: A Practical Guide will be an oft-used reference for geneticists, molecular engineers, and molecular biologists.
Photoacoustic Reporter Gene Imaging And Optical Coherence Computed Tomography
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Author : Li Li
language : en
Publisher:
Release Date : 2010
Photoacoustic Reporter Gene Imaging And Optical Coherence Computed Tomography written by Li Li and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Electronic dissertations categories.
Molecular Imaging Ii
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Author : Wolfhard Semmler
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-07-15
Molecular Imaging Ii written by Wolfhard Semmler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-07-15 with Medical categories.
The aim of this textbook of molecular imaging is to provide an up to date review of this rapidly growing field and to discuss basic methodological aspects necessary for the interpretation of experimental and clinical results. Emphasis is placed on the interplay of imaging technology and probe development, since the physical properties of the imaging approach need to be closely linked with the biologic application of the probe (i.e. nanoparticles and microbubbles). Various chemical strategies are discussed and related to the biologic applications. Reporter-gene imaging is beeing addressed not only in experimental protocols, but also first clinical applications are discussed. Finally, strategies of imaging to characterize apoptosis and angiogenesis are described and discussed in the context of possible clinical translation.
Multimodality Imaging Of Reporter Gene Expression Using A Novel Fusion Vector In Living Cells And Animals
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Author :
language : en
Publisher:
Release Date : 2011
Multimodality Imaging Of Reporter Gene Expression Using A Novel Fusion Vector In Living Cells And Animals written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
Novel double and triple fusion reporter gene constructs harboring distinct imagable reporter genes are provided, as well as applications for the use of such double and triple fusion constructs in living cells and in living animals using distinct imaging technologies.
Reporter Gene Assays
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Author : Robert Damoiseaux
language : en
Publisher: Humana Press
Release Date : 2018-04-19
Reporter Gene Assays written by Robert Damoiseaux and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-19 with Medical categories.
This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs. Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interested in learning more about reporter genes.
Imaging Reporter Transgene Expression In Living Subjects Using Positron Emission Tomography
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Author : Shahriar Yaghoubi
language : en
Publisher:
Release Date : 2002
Imaging Reporter Transgene Expression In Living Subjects Using Positron Emission Tomography written by Shahriar Yaghoubi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with categories.
The Development Of Reporter Genes For In Vivo Imaging
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Author : Peter Stephen Patrick
language : en
Publisher:
Release Date : 2014
The Development Of Reporter Genes For In Vivo Imaging written by Peter Stephen Patrick and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.