The Golgi Complex Methods And Protocols
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The Golgi Complex Methods And Protocols
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Author : William J. Brown
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-10-07
The Golgi Complex Methods And Protocols written by William J. Brown and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-07 with Science categories.
Focusing on new methods and techniques developed to address longstanding questions about the Golgi complex, this volume explores a diverse set of chapters, ranging from live and fixed cell imaging techniques toin vitro biochemical reconstitution systems. Each chapter provides a detailed set of specific instructions, which should enable anyone to successfully complete the assays. Written for the highly successful Methods in Molecular Biology series program, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips from the experts on troubleshooting and avoiding known pitfalls. Practical and detailed, The Golgi Complex: Methods and Protocols will aid both new and established researchers in the field by providing strong hands-on instructions that can be directly applied to their research programs.iv>
Golgi
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Author : Yanzhuang Wang
language : en
Publisher: Springer Nature
Release Date : 2022-12-13
Golgi written by Yanzhuang Wang and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-12-13 with Science categories.
This volume provides readers with a collection of new and classical methods, techniques, and applications used to address enduring questions about the structure and functions of the Golgi complex. The chapters in this volume cover diverse topics ranging from model systems; live and fixed cell imaging techniques; in vitro biochemical reconstitution systems; and specific methods developed to study Golgi formation, maintenance, and functions under physiological and pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Golgi: Methods and Protocols is a valuable tool for researchers in the field who wish to explore new areas of Golgi biology and for new investigators interested in exploring Golgi structure and function.
Methods For Analysis Of Golgi Complex Function
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Author :
language : en
Publisher: Elsevier
Release Date : 2013-11-26
Methods For Analysis Of Golgi Complex Function written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-26 with Science categories.
This new volume of Methods in Cell Biology looks at methods for analyzing of golgi complex function. Chapters cover such topics as in vitro reconstitution systems, fluorescence-based analysis of trafficking in mammalian cells and high content screening. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
The Golgi Apparatus
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Author : Eric Berger
language : en
Publisher: Birkhäuser
Release Date : 2012-12-06
The Golgi Apparatus written by Eric Berger and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
In 1898 Camillo Golgi reported his newly observed intracellular structure, the apparato reticolare interno, now universally known as the Golgi Apparatus. The method he used was an ingenious histological technique (La reazione nera) which brought him fame for the discovery of neuronal networks and culminated in the award of the Nobel Prize for Physiology and Medicine in 1906. This technique, however, was not easily reproducible and led to a long-lasting controversy about the reality of the Golgi apparatus. Its identification as a ubiquitous organelle by electron microscopy turned out to be the breakthrough and incited an enormous wave of interest in this organelle at the end of the sixties. In recent years immunochemical techniques and molecular cloning approaches opened up new avenues and led to an ongoing resurgence of interest. The role of the Golgi apparatus in modifying, broadening and refining the structural information conferred by transcription/translation is now generally accepted but still incompletely understood. During the coming years, this topic certainly will remain center stage in the field of cell biology. The centennial of the discovery of this fascinating organelle prompted us to edit a new comprehensive book on the Golgi apparatus whose complexity necessitated the contributions of leading specialists in this field. This book is aimed at a broad readership of glycobiologists as well as cell and molecular biologists and may also be interesting for advanced students of biology and life sciences.
The Golgi Apparatus
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Author : Alexander A. Mironov
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-03-04
The Golgi Apparatus written by Alexander A. Mironov and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-03-04 with Science categories.
This book summarizes all new data obtained after development of methods of Golgi complex sub fractionation, molecular biology and microscopy. It collects the full range of expertise, different points of view and different approaches. The book is devoted to molecular modes of the function of the Golgi apparatus as a whole, taking into account all experimental data. The book aims to make the functional organization of the Golgi apparatus more understandable.
Unconventional Protein Secretion
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Author : Andrea Pompa
language : en
Publisher: Humana
Release Date : 2018-06-14
Unconventional Protein Secretion written by Andrea Pompa and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-06-14 with Science categories.
This volume presents relevant background information to understanding the molecular basis governing unconventional protein secretion (UPS), and in particular explores the latest techniques and protocols that have been successfully applied for the study of this topic. Detailed chapters include an overview of conventional and unconventional secretory pathways along with multidisciplinary approaches and methods used for UPS analysis in different organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Unconventional Protein Secretion: Methods and Protocols will be useful for all interested in the secretory pathway field as well as applications in cell biology, cell development, biomedical research, and healthcare.
The Golgi Apparatus
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Author : W.G. Whaley
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
The Golgi Apparatus written by W.G. Whaley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
A comprehensive review of the Golgi apparatus and its functioning would require a multi-volume publication and not a monograph and it would be so repetitious as to discourage the reader. The requirement at this stage is for a reinterpretation of the character and functioning of this organelle since the last major interpretations have concentrated on its role in secretion and it has now been shown to be a component of essentially all cells whether or not they have been traditionally emphasized as secreting cells. As a conse quence the efforts have been placed on the common characteristics of the organelle, a postulate concerning its functioning in cells generally, and the details of variations where these seem important. The major acknowledgment of assistance in compiling the material must go to the investigators whose contributions, sometimes positive and sometimes of a character to spur additional investigations, allowed the development of this postulate. The paper has been prepared with the detailed assistance of Dr. MARIANNE DAUWALDER who, by her own studies and her insight into the significance of other studies, has been a working partner of many years in the development of a general hypothesis and whose knowledge of investiga tions of the Golgi apparatus is great enough to let her call attention to instances of support and contention with the general functional hypothesis that has been involved.
The Golgi Apparatus
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Author : James Morré
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-12-11
The Golgi Apparatus written by James Morré and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-12-11 with Science categories.
The Golgi apparatus (GA) is typically comprised of a series of five to eight cup-shaped, membrane-covered sacs called cisternae that look something like a stack of deflated balloons. The GA is often considered the "distribution and shipping department" for the cell's chemical products. This book traces the first 100 years of GA discovery from the first published accounts from Pavia, Italy, in 1898 to the Centenary Celebration in Pavia, Italy, in 1998 to our most recent discoveries. It summarizes the past 50 years beginning with the modern era of GA discovery, leading up to the present era with almost exclusive focus on molecular biology.
Golgi Apparatus
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Author : Christopher J. Hawkins
language : en
Publisher:
Release Date : 2011
Golgi Apparatus written by Christopher J. Hawkins and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Golgi apparatus categories.
The Golgi apparatus is an organelle found in most eukaryotic cells. The primary function of the Golgi apparatus is to process and package macromolecules, such as proteins and lipids, after their synthesis and before they make their way to their destination. This book presents topical research data in the study of Golgi apparatus, including Golgi organization and stress sensing; signaling pathways controlling mitotic Golgi breakdown in mammalian cells; the role of Golgi apparatus in the biological mechanisms of hypericin-mediated photodynamic therapy; the role of the Trans-Golgi Network (TGN) in the sorting of nonenzymic lysosomal proteins; and the mechanisms involving the role of Golgi apparatus alteration in neurological disorders triggered by manganese.
Electron Microscopy Methods And Protocols
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Author : M. A. Nasser Hajibagheri
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Electron Microscopy Methods And Protocols written by M. A. Nasser Hajibagheri and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).