The Molecular Biology Of Baculoviruses
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The Molecular Biology Of Baculoviruses
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Author : Walter Doerfler
language : en
Publisher:
Release Date : 2014-08-15
The Molecular Biology Of Baculoviruses written by Walter Doerfler and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-08-15 with categories.
Biology Of Baculoviruses
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Author : Robert R. Granados
language : en
Publisher: Springer
Release Date : 1986
Biology Of Baculoviruses written by Robert R. Granados and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1986 with Science categories.
V.1 - Biological properties and molecular biology: Historical perspectives; Taxonomy and identification of baculoviruses; Ultrastructure of baculoviruses; In vivo infection and replication of baculoviruses; In vitro replication of baculoviruses; Baculovirus structural proteins and protein synthesis; Current developments in baculovirus serology; Persistent baculovirus infections; Specificity and safety of baculoviruses; Evolution of occluded baculoviruses; The genetics of baculoviruses; Organization and expression of the baculovirus genome; v.2 - Practical application for insect control: In vivo and in vitro bioassay methods for baculoviruses; In vivo production of baculoviruses; Cell culture methods for large-scale propagation of baculoviruses; Ecology and ens, chemical pesticides, and parasitoids; Formulation and application of baculoviruses; Use of baculoviruses in pest management programs; Registration of baculoviruses as pesticides; Practical factors influencing the utilization of baculoviruses as pesticides; Insect resistance to baculoviruses.
The Baculoviruses
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Author : Lois K. Miller
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29
The Baculoviruses written by Lois K. Miller and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Medical categories.
The past decade has witnessed an explosion of information on the molecular biology of insect viruses and a frenzy of activity in applying this information to medicine and agriculture. Genetically engineered baculoviruses are presently being tested for commercial use as pesticides, and the study of such viruses is also revealing remarkable insights into basic cellular processes such as apoptosis. This comprehensive volume provides readers with knowledge of basic and applied baculovirology so that current literature in the field can be appreciated.
Baculovirus And Insect Cell Expression Protocols Methods In Molecular Biology
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Author : David W. Murhammer
language : en
Publisher:
Release Date : 2007
Baculovirus And Insect Cell Expression Protocols Methods In Molecular Biology written by David W. Murhammer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.
"Baculovirus Expression Protocols" is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the "Methods in Molecular Biology" series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of "Baculovirus Expression Protocols" provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.
Baculovirus And Insect Cell Expression Protocols
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Author : David W. Murhammer
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-04-15
Baculovirus And Insect Cell Expression Protocols written by David W. Murhammer and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-15 with Medical categories.
The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.
The Baculoviruses
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Author : Lois K Miller
language : en
Publisher: Springer
Release Date : 2014-01-15
The Baculoviruses written by Lois K Miller and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-15 with categories.
Baculovirus Molecular Biology 4th Edition
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Author :
language : en
Publisher:
Release Date : 2019
Baculovirus Molecular Biology 4th Edition written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with categories.
Baculovirus Expression Vectors
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Author : David R. O'Reilly
language : en
Publisher:
Release Date : 1994
Baculovirus Expression Vectors written by David R. O'Reilly and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Baculoviruses categories.
Baculoviruses have proven to be the most powerful and versatile eukaryotic expression vectors available. This unique laboratory manual is designed to help both beginning and experienced researchers construct and use baculovirus vector systems. It simplifies selection of the most appropriatebaculovirus vector design for a given problem, then describes each step of the implementation process--from vector construction to large-scale protein production. The book provides an understanding of how the vectors work; a biological overview of cells, viruses, plasmids, and promoters; guidelinesfor choosing optimum vectors; protocols for growing insect cells and recombinant viruses; methods of analyzing protein products and scaling up protein production; techniques for producing proteins in insect larvae; and easy-to-use maps charting available expression vectors. This comprehensiveapproach has many benefits for researchers and students alike. It allows them to understand how and why the vector system works and offers a rapid comparison of options for choosing the right virus, plasmid or promoter for vector design and construction, with a minimum amount of lost time. Themanual is an invaluable resource for every individual engaged in the production of proteins for any purpose.
Baculovirus Expression Protocols
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Author : Christopher D. Richardson
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Baculovirus Expression Protocols written by Christopher D. Richardson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Baculovirus Expression Protocols offers both industrial and university-based researchers a comprehensive comilation of the latest baculovirus techniques along with step-by-step instructions and time-saving techniques. The contributors-leading authorities in the field-present the assorted expression plasmids currently in use, guide the reader through the process of generating and selecting recombinant virus, and describe specific examples of recombinant protein production and purification. The emphasis is on alternative and simpler screening techniques for the selection of recombinant baculovirus. The book also surveys the various insect cell lines currently compatible with the baculovirus system. Highlights include production of recombinant virus using linearized DNA and vectors that contain a b-galactosidase indicator and a complete list of expression vectors currently available. Detailed descriptions for the scale-up of protein production using spinner flasks, bioreactors, and insect larvae are also included. Baculovirus Expression Protocols offers both industrial and university-based researchers an outstanding collection of reproducible, step-by-step laboratory protocols. It will immediately become indispensable for anyone working with baculoviruses and their application in the expression of recombinant proteins in insect cells.
Baculovirus Molecular Biology
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Author : George F. Rohrmann
language : en
Publisher:
Release Date : 2011
Baculovirus Molecular Biology written by George F. Rohrmann and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Baculoviruses categories.
This book is based on a project to completely annotate what is known about the function of the 1̃50 genes encoded by the genome of the most commonly investigated baculovirus, AcMNPV. Once the annotation was completed (summarized in Chapter 12), the material was organized into a series of chapters integrating the role of the gene products in the biology of major processes central to the replication and pathology of baculoviruses. Chapters on taxonomy, the application of baculoviruses as insecticides, the molecular basis for the remarkable ability of these viruses to express genes at high levels, and the interrelationships of baculovirus and insect retroviruses are also included. The chapters are accompanied by 41 figures, 14 tables and a glossary of common terms used in baculovirology.