Baculovirus Expression Vectors
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Baculovirus Expression Vectors
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Author : David R. O'Reilly
language : en
Publisher:
Release Date : 1994
Baculovirus Expression Vectors written by David R. O'Reilly and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Medical categories.
Baculoviruses have proven to be the most powerful and versatile eukaryotic expression vectors available. This unique laboratory manual is designed to help both beginning and experienced researchers construct and use baculovirus vector systems. It simplifies selection of the most appropriate baculovirus vector design for a given problem, then describes each step of the implementation process--from vector construction to large-scale protein production. The book provides an understanding of how the vectors work; a biological overview of cells, viruses, plasmids, and promoters; guidelines for choosing optimum vectors; protocols for growing insect cells and recombinant viruses; methods of analyzing protein products and scaling up protein production; techniques for producing proteins in insect larvae; and easy-to-use maps charting available expression vectors. This comprehensive approach has many benefits for researchers and students alike. It allows them to understand how and why the vector system works and offers a rapid comparison of options for choosing the right virus, plasmid or promoter for vector design and construction, with a minimum amount of lost time. The manual is an invaluable resource for every individual engaged in the production of proteins for any purpose.
The Baculoviruses
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Author : Lois K Miller
language : en
Publisher: Springer
Release Date : 2014-01-15
The Baculoviruses written by Lois K Miller and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-15 with categories.
Baculovirus Expression Protocols
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Author : Christopher D. Richardson
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Baculovirus Expression Protocols written by Christopher D. Richardson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Baculovirus Expression Protocols offers both industrial and university-based researchers a comprehensive comilation of the latest baculovirus techniques along with step-by-step instructions and time-saving techniques. The contributors-leading authorities in the field-present the assorted expression plasmids currently in use, guide the reader through the process of generating and selecting recombinant virus, and describe specific examples of recombinant protein production and purification. The emphasis is on alternative and simpler screening techniques for the selection of recombinant baculovirus. The book also surveys the various insect cell lines currently compatible with the baculovirus system. Highlights include production of recombinant virus using linearized DNA and vectors that contain a b-galactosidase indicator and a complete list of expression vectors currently available. Detailed descriptions for the scale-up of protein production using spinner flasks, bioreactors, and insect larvae are also included. Baculovirus Expression Protocols offers both industrial and university-based researchers an outstanding collection of reproducible, step-by-step laboratory protocols. It will immediately become indispensable for anyone working with baculoviruses and their application in the expression of recombinant proteins in insect cells.
The Baculovirus Expression System
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Author : Linda King
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
The Baculovirus Expression System written by Linda King and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Social Science categories.
The decision to write a book about the practical aspects of the baculovirus expression system stems from the numerous phone calls for help we have had, and from the many visitors to our labora tories requiring assistance to find the elusive polyhedrin-negative virus containing their favourite gene. We have also organized two expression system workshops and from the manuals we wrote for these, it seemed a logical progression to extend them into book form. We appreciate that those who are 'old-hands' at the baculovirus expression system may have differing views on some of our procedures, but the methods in this book are presented in the light of our own experiences in the laboratory and from our practical workshops, and we hope that the book will be especially useful to those new to the system. The first three chapters give the background information to the baculovirus expression system, and includes advice on how to choose the right transfer vector and discusses the various methods that are available to select recombinant viruses. The practical chapters concentrate on those aspects which are novel to the baculovirus system (insect cell culture, virus amplification and titration, etc. ) and, in general, leave the standard molecular biological techniques to the other excellent laboratory manuals that are available. However, for completeness sake and to avoid constant reference to other manuals, we have included brief details of some standard techniques where they are integral to the success of the baculovirus protocols.
Comprehensive Molecular Insect Science Reproduction And Development
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Author : Lawrence Irwin Gilbert
language : en
Publisher:
Release Date : 2005
Comprehensive Molecular Insect Science Reproduction And Development written by Lawrence Irwin Gilbert and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Science categories.
Comprehensive reference text on molecular insect science. Includes coverage of developments, achievements and new technologies in modern insect science.
Insect Cell Culture Engineering
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Author : Mattheus F. A. Goosen
language : en
Publisher: CRC Press
Release Date : 2020-07-24
Insect Cell Culture Engineering written by Mattheus F. A. Goosen and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-24 with Science categories.
Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.
Trends In Insect Molecular Biology And Biotechnology
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Author : Dhiraj Kumar
language : en
Publisher: Springer
Release Date : 2018-04-03
Trends In Insect Molecular Biology And Biotechnology written by Dhiraj Kumar and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-03 with Science categories.
This book provides an overview on the basics in insect molecular biology and presents the most recent developments in several fields such as insect genomics and proteomics, insect pathology and applications of insect derived compounds in modern research. The book aims to provide a common platform for the molecular entomologist to stimulate further research in insect molecular biology and biotechnology. Insects are one of the most versatile groups of the animal kingdom. Due to their large population sizes and adaptability since long they attract researchers’ interest as efficient resource for agricultural and biotechnological purposes. Several economically important insects such as Silkworms, Honey Bee, Lac and Drosophila or Termites were established as invertebrate model organisms. Starting with the era of genetic engineering, a broad range of molecular and genetic tools have been developed to study the molecular biology of these insects in detail and thus opened up a new horizon for multidisciplinary research. Nowadays, insect derived products are widely used in biomedical and biotechnology industries. The book targets researchers from both academia and industry, professors and graduate students working in molecular biology, biotechnology and entomology.
Insect Viruses
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Author : Byrony C Bonning
language : en
Publisher: Academic Press
Release Date : 2006-09-22
Insect Viruses written by Byrony C Bonning and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-09-22 with Science categories.
Baculoviruses are perhaps unique among viruses in the breadth of their biotechnological applications: these insect specific viruses are used not only for insect pest management purposes, but also as laboratory research tools for production of recombinant proteins and for protein display, and as potential vectors for human gene therapy. In addition to highlighting recent advances, this volume provides a comprehensive review of the biotechnological applications of these and other insect viruses in both the academic and private sectors.
Cell And Tissue Reaction Engineering
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Author : Regine Eibl
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-09-30
Cell And Tissue Reaction Engineering written by Regine Eibl and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-09-30 with Science categories.
The completion of the Human Genome Project and the rapid progress in cell bi- ogy and biochemical engineering, are major forces driving the steady increase of approved biotech products, especially biopharmaceuticals, in the market. Today mammalian cell products (“products from cells”), primarily monoclonals, cytokines, recombinant glycoproteins, and, increasingly, vaccines, dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells (“cells as product”) for regenerative medicine have also been introduced in the market. Their efficient production requires comprehensive knowledge of biological as well as biochemical mammalian cell culture fundamentals (e.g., cell characteristics and metabolism, cell line establishment, culture medium optimization) and related engineering principles (e.g., bioreactor design, process scale-up and optimization). In addition, new developments focusing on cell line development, animal-free c- ture media, disposables and the implications of changing processes (multi-purpo- facilities) have to be taken into account. While a number of excellent books treating the basic methods and applications of mammalian cell culture technology have been published, only little attention has been afforded to their engineering aspects. The aim of this book is to make a contribution to closing this gap; it particularly focuses on the interactions between biological and biochemical and engineering principles in processes derived from cell cultures. It is not intended to give a c- prehensive overview of the literature. This has been done extensively elsewhere.
Baculovirus And Insect Cell Expression Protocols
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Author : David W. Murhammer
language : en
Publisher: Humana Press
Release Date : 2007-07-06
Baculovirus And Insect Cell Expression Protocols written by David W. Murhammer and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-07-06 with Science categories.
Baculovirus Expression Protocols, Second Edition, provides the detailed steps required to perform the techniques involved with the use of baculoviruses and insect cell culture and discusses problems that may be encountered. This newly-revised and updated edition provides a cutting edge step-by-step guide for biochemists, molecular biologists, biochemical engineers and others using the BEVS and/or insect cells for producing recombinant proteins. Furthermore, Baculovirus Expression Protocols, Second Edition, provides assistance to scientists and engineers interested in developing and producing baculovirus insecticides. Authors list all available insect cell lines and provide methods for isolating new cell lines. The procedures involved in producing products, both at laboratory scale and large scale, are discussed, as well as the production in insect larvae. It is hoped that this volume will not only aid the user in successfully completing the tasks described herein, but will also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture. Timely and thorough, this volume will become an essential reference for anyone using baculoviruses and insect cell culture.