The Pcr Revolution
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The Pcr Revolution
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Author : Stephen A. Bustin
language : en
Publisher: Cambridge University Press
Release Date : 2010
The Pcr Revolution written by Stephen A. Bustin and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Science categories.
Examines the latest innovations and the overall impact of PCR on areas of molecular research.
Applications Of Polymerase Chain Reaction
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Author : Sarika Garg
language : en
Publisher: Arcler Press
Release Date : 2017-11
Applications Of Polymerase Chain Reaction written by Sarika Garg and has been published by Arcler Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-11 with categories.
The polymerase chain reaction (PCR) is a widely used molecular biology technique. It was invented in 1983 by Kary Mullis, who was awarded a Nobel Prize in Chemistry in 1993 for his innovation. Since its introduction, the PCR technology has brought a true revolution in scientific research involving applied areas such as diagnosis and genetic improvements for plants and animals. PCR is a method of replicating deoxyribonucleic acid (DNA) by the selective amplification of a target region of a DNA molecule. Only few cycles of PCR can generate millions of copies of a particular DNA sequence quickly and precisely. Routinely, a PCR reaction consists of three distinct steps- denaturation, annealing and extension. In the first step of denaturation (at 95°C), double stranded DNA denatures into two single strands. In the annealing step (at 56°C), short complementary sequences of DNA known as primers, anneal to the single stranded target DNA. In the final step of extension, temperature is raised to around 72℃. DNA polymerase extends the primer to form a second complimentary strand of DNA. The elaboration of a PCR technique for amplification and for analyzing even very small amounts of DNA or a damaged DNA has generated wide applications in analysis of gene, diagnosis of several genetic diseases and detection of infectious agents like viruses, bacteria, fungi, protozoa, etc. Another beneficial application of PCR is in the generation of a genetic fingerprint. PCR is also used to monitor pathogens in the environment. PCR is an imperative technique for research in molecular biology such as in cloning procedure which has considerable potential in forensic science. PCR has numerous applications in various other fields such as agriculture sciences, personalized medicine, archeology, etc. This book gathers the recently published works related to PCR and its applications. The first chapter highlights the introduction to polymerase chain reaction. The second chapter features the application of reverse transcriptase-polymerase chain reaction (RT-PCR) in the rapid detection of pathogen in water samples. In chapter 3, discussion is on the PCR methods useful for accurate and rapid screening of the genetically modified organisms in foods. PCR applications in forensic science are described in chapter 4. Application of PCR for the diagnosis of ankylosing spondylitis is discussed in chapter 5. In chapters 6-12, use of PCR technique for the detection of pathogens and diagnosis of infectious diseases is described. PCR methods for the detection of Alzheimer's disease-related single nucleotide polymorphism and cancer related genes are illustrated in chapters 13 and 14, respectively. Chapter 15 discusses the PCR in exploring endodontic infections. Analysis of the archaeological skeletal remains using PCR is described in the last chapter of the book. I hope that this book will be advantageous for students, researchers, teachers, and industrial experts.
Laboratory Yellowstone And The Dna Revolution
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Author : Robert Lindstrom
language : en
Publisher:
Release Date : 2017-07
Laboratory Yellowstone And The Dna Revolution written by Robert Lindstrom and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07 with categories.
Biotechnology
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Author : David P. Clark
language : en
Publisher: Academic Press
Release Date : 2010-07-21
Biotechnology written by David P. Clark and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-07-21 with Medical categories.
Unlike most biotechnology textbooks, Dr. David P. Clark's Biotechnology approaches modern biotechnology from a molecular basis, which grew out of the increasing biochemical understanding of physiology. Using straightforward, less-technical jargon, Clark manages to introduce each chapter with a basic concept that ultimately evolves into a more specific detailed principle. This up-to-date text covers a wide realm of topics, including forensics and bioethics, using colorful illustrations and concise applications. This book will help readers understand molecular biotechnology as a scientific discipline, how the research in this area is conducted, and how this technology may impact the future. · Up-to-date text focuses on modern biotechnology with a molecular foundation · Basic concepts followed by more detailed, specific applications · Clear, color illustrations of key topics and concepts · Clearly written without overly technical jargon or complicated examples
Pcr Technology
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Author : Tania Nolan
language : en
Publisher: CRC Press
Release Date : 2013-06-13
Pcr Technology written by Tania Nolan and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-13 with Law categories.
PCR’s simplicity as a molecular technique is, in some ways, responsible for the huge amount of innovation that surrounds it, as researchers continually think of new ways to tweak, adapt, and re-formulate concepts and applications. PCR Technology: Current Innovations, Third Edition is a collection of novel methods, insights, and points of view that provides a critical and timely reference point for anyone wishing to use this technology. Topics in this forward-thinking volume include: The purification and handling of PCR templates The effect of the manufacture and purification of the oligonucleotide on PCR behavior Optimum buffer composition Probe options The design and optimization of qPCR assays Issues surrounding the development and refinement of instrumentation Effective controls to protect against uncertainties due to reaction variability Covering all aspects of PCR and real-time PCR, the book contains detailed protocols that make it suitable as both a reference and an instruction manual. Each chapter presents detailed guidelines as well as helpful hints and tips supplied by authors who are recognized experts in their fields. In addition to descriptions of current technology and best practices, the book also provides information about new developments in the PCR arena.
Calculating The Secrets Of Life
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Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 1995-04-06
Calculating The Secrets Of Life written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-04-06 with Science categories.
As researchers have pursued biology's secrets to the molecular level, mathematical and computer sciences have played an increasingly important roleâ€"in genome mapping, population genetics, and even the controversial search for "Eve," hypothetical mother of the human race. In this first-ever survey of the partnership between the two fields, leading experts look at how mathematical research and methods have made possible important discoveries in biology. The volume explores how differential geometry, topology, and differential mechanics have allowed researchers to "wind" and "unwind" DNA's double helix to understand the phenomenon of supercoiling. It explains how mathematical tools are revealing the workings of enzymes and proteins. And it describes how mathematicians are detecting echoes from the origin of life by applying stochastic and statistical theory to the study of DNA sequences. This informative and motivational book will be of interest to researchers, research administrators, and educators and students in mathematics, computer sciences, and biology.
Human Genetics
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Author : Edwin H. McConkey
language : en
Publisher: Jones & Bartlett Learning
Release Date : 1993
Human Genetics written by Edwin H. McConkey and has been published by Jones & Bartlett Learning this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Medical categories.
Begins with molecular characterization of the human genome (rather than the conventional descriptions of Mendelian inheritance, pedigree analysis, and chromosome abnormalities), and maintains this emphasis on understanding human genetics in molecular terms throughout. Suitable as a text for biology
The Polymerase Chain Reaction
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Author : Kary B. Mullis
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-02-02
The Polymerase Chain Reaction written by Kary B. Mullis and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-02-02 with Medical categories.
James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...
Molecular Techniques In Food Biology
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Author : Aly Farag El Sheikha
language : en
Publisher: John Wiley & Sons
Release Date : 2018-01-02
Molecular Techniques In Food Biology written by Aly Farag El Sheikha and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-02 with Technology & Engineering categories.
Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity & Traceability explores all aspects of microbe-food interactions, especially as they pertain to food safety. Traditional morphological, physiological, and biochemical techniques for the detection, differentiation, and identification of microorganisms have severe limitations. As an alternative, many of those responsible for monitoring food safety are turning to molecular tools for identifying foodborne microorganisms. This book reviews the latest molecular techniques for detecting, identifying, and tracing microorganisms in food, addressing both good foodborne microbes, such as those used for fermentation and in probiotics, and harmful ones responsible for foodborne illness and food quality control problems. Molecular Techniques in Food Biology: Safety, Biotechnology, Authenticity & Traceability brings together contributions by leading international authorities in food biology from academe, industry, and government. Chapters cover food microbiology, food mycology, biochemistry, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and food science and technology. Throughout, special emphasis is placed on novel molecular techniques relevant to food biology research and for monitoring and assessing food safety and quality. Brings together contributions from scientists at the leading edge of the revolution in molecular food biology Explores how molecular techniques can satisfy the dire need to deepen our understanding of how microbial communities develop in foods of all types and in all forms Covers all aspects of food safety and hygiene, microbial ecology, food biotechnology and bio-processing, food authenticity, food origin traceability, and more Fills a yawning gap in the world literature on food traceability using molecular techniques This book is an important working resource for professionals in agricultural, food science, biomedicine, and government involved in food regulation and safety. It is also an excellent reference for advanced students in agriculture, food science and food technology, biochemistry, microbiology, and biotechnology, as well as academic researchers in those fields.
Real Time Pcr
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Author : M Dorak
language : en
Publisher: Garland Science
Release Date : 2007-01-24
Real Time Pcr written by M Dorak and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-01-24 with Medical categories.
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.