Pcr Technology


Pcr Technology
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Pcr Technology


Pcr Technology
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Author : Tania Nolan
language : en
Publisher: CRC Press
Release Date : 2013-06-13

Pcr Technology written by Tania Nolan and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-13 with Law categories.


PCR’s simplicity as a molecular technique is, in some ways, responsible for the huge amount of innovation that surrounds it, as researchers continually think of new ways to tweak, adapt, and re-formulate concepts and applications. PCR Technology: Current Innovations, Third Edition is a collection of novel methods, insights, and points of view that provides a critical and timely reference point for anyone wishing to use this technology. Topics in this forward-thinking volume include: The purification and handling of PCR templates The effect of the manufacture and purification of the oligonucleotide on PCR behavior Optimum buffer composition Probe options The design and optimization of qPCR assays Issues surrounding the development and refinement of instrumentation Effective controls to protect against uncertainties due to reaction variability Covering all aspects of PCR and real-time PCR, the book contains detailed protocols that make it suitable as both a reference and an instruction manual. Each chapter presents detailed guidelines as well as helpful hints and tips supplied by authors who are recognized experts in their fields. In addition to descriptions of current technology and best practices, the book also provides information about new developments in the PCR arena.



Pcr Technology


Pcr Technology
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Author : Henry Erlich
language : en
Publisher: Springer
Release Date : 2015-12-31

Pcr Technology written by Henry Erlich and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-12-31 with Science categories.


This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.



Pcr Technology


Pcr Technology
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Author : Thomas Weissensteiner
language : en
Publisher: CRC Press
Release Date : 2003-11-13

Pcr Technology written by Thomas Weissensteiner and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-11-13 with Medical categories.


A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of



Pcr Technology


Pcr Technology
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Author : Henry A. Erlich
language : en
Publisher: Oxford University Press, USA
Release Date : 1992

Pcr Technology written by Henry A. Erlich and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with DNA categories.


Polymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for.



Principles And Technical Aspects Of Pcr Amplification


Principles And Technical Aspects Of Pcr Amplification
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Author : Elizabeth van Pelt-Verkuil
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-03-14

Principles And Technical Aspects Of Pcr Amplification written by Elizabeth van Pelt-Verkuil and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-03-14 with Science categories.


Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).



Pcr Technology


Pcr Technology
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Author : Hugh G. Griffin
language : en
Publisher: CRC Press
Release Date : 2004-11-23

Pcr Technology written by Hugh G. Griffin and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-11-23 with Science categories.


A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. A bestseller in its first edition, this second edition provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution.



Pcr Technology


Pcr Technology
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Author : Henry Erlich
language : en
Publisher: Palgrave Macmillan
Release Date : 1989-10-01

Pcr Technology written by Henry Erlich and has been published by Palgrave Macmillan this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-10-01 with Science categories.


This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.



Pcr Protocols


Pcr Protocols
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Author : Bruce A. White
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02

Pcr Protocols written by Bruce A. White and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.


PCR has been successfully utilized in every facet of basic, cli- cal, and applied studies of the life sciences, and the impact that PCR has had on life science research is already staggering. C- comitant with the essentially universal use of PCR has been the creative and explosive development of a wide range of PCR-based techniques and applications. These increasingly numerous pro- cols have each had the general effect of facilitating and acceler- ing research. Because PCR technology is relatively easy and inexpensive, PCR applications are well within the reach of every research lab. In this sense, PCR has become the "equalizer" between "small" and "big" labs, since its use makes certain projects, especially those related to molecular cloning, now far more feasible for the small lab with a modest budget. This new volume on PCR Protocols does not attempt the impossible task of representing all PCR-based protocols. Rather, it presents a range of protocols, both analytical and preparative, that provide a solid base of knowledge on the use of PCR in many c- mon research problems. The first six chapters provide some basic information on how to get started. Chapters 7-19 represent primarily analytical uses of PCR, both for simple DNA and RNA detection, as well as for more complex analyses of nucleic acid (e. g. , DNA footprin ting, RNA splice site localization). The remaining chapters represent "synthetic," or preparative, uses of PCR.



Polymerase Chain Reaction


Polymerase Chain Reaction
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Author : Mark A. Behlke
language : en
Publisher:
Release Date : 2019-07

Polymerase Chain Reaction written by Mark A. Behlke and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-07 with Science categories.


This indispensable manual is a compilation of review articles written by experts in the field of PCR technology. It is a recommended purchase for all microbiology and molecular biology laboratories and university libraries.



The Pcr Revolution


The Pcr Revolution
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Author : Stephen A. Bustin
language : en
Publisher: Cambridge University Press
Release Date : 2010

The Pcr Revolution written by Stephen A. Bustin and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Science categories.


Examines the latest innovations and the overall impact of PCR on areas of molecular research.