Tissue Engineering Methods And Protocols
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Tissue Engineering Methods And Protocols
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Author : Jeffrey R. Morgan
language : en
Publisher: Springer Science & Business Media
Release Date : 1998-09-28
Tissue Engineering Methods And Protocols written by Jeffrey R. Morgan and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-09-28 with Technology & Engineering categories.
In recent years, the field of tissue engineering has begun, in part, to c- lesce around the important clinical goal of developing substitutes or repla- ments for defective tissues or organs. These efforts are focused on many tissues including skin, cartilage, liver, pancreas, bone, blood, muscle, the vascu- ture, and nerves. There is a staggering medical need for new and effective treatments for acquired as well as inherited defects of organs/tissues. Tissue engineering is at the interface of the life sciences, engineering, and clinical medicine and so draws upon advances in cell and molecular biology, mate- als sciences, and surgery, as well as chemical and mechanical engineering. Such an interdisciplinary field requires a broad knowledge base as well as the use of a wide assortment of methods and approaches. It is hoped that by bringing together these protocols, this book will help to form connections - tween the different disciplines and further stimulate the synergism underlying the foundation of the tissue engineering field.
Biomaterials For Tissue Engineering
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Author : Kanika Chawla
language : en
Publisher: Humana
Release Date : 2019-05-05
Biomaterials For Tissue Engineering written by Kanika Chawla and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-05 with Science categories.
This volume provides protocols for the generation of various biomaterials for tissue engineering and regenerative medicine applications. The chapters in this book include a look at a range of biomaterials including hydrogels and other matrices (natural, synthetic, self-healing) for various applications including drug and gene delivery, surface modification and functionalization of biomaterials. In addition, techniques described include those for controlling biomaterial geometry, such as three-dimensional printing and electrospinning. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Biomaterials for Tissue Engineering: Methods and Protocols is a valuable resource for scientists and engineers interested in this vital field of study.
Vascular Tissue Engineering
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Author : Feng Zhao
language : en
Publisher: Humana
Release Date : 2022-10-02
Vascular Tissue Engineering written by Feng Zhao and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-10-02 with Science categories.
This volume explores the latest techniques used to study the field of tissue engineered vascular grafts (TEVGs). The chapters in this book cover a wide array of topics such as deriving vascular cells from monocytes and induced pluripotent stem cells; engineering vascular grafts using various biomaterials and stem cells, stem cell-derived, or primary vascular cells; biomaterial modification by anticoagulation molecules; vascular bioengineering technologies such as 3D bioprinting; and fabrication of TEVGs with different geometry and multiphase structures. This book also features protocols for grafting and evaluation of vascular grafts in animal models, vascular imaging in animals, and the quantification of blood vessel permeability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Vascular Tissue Engineering: Methods and Protocols is a valuable resource for biomedical engineers, cell biologists, vascular surgeons, doctors, and nurses.
Skin Tissue Engineering
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Author : Sophie Böttcher-Haberzeth
language : en
Publisher: Springer Nature
Release Date : 2019-05-30
Skin Tissue Engineering written by Sophie Böttcher-Haberzeth and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-05-30 with Science categories.
This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Skin Tissue Engineering: Methods and Protocols serves as a vital aid to basic and clinical researchers such as biologists, physicians, and biomedical engineers working with and being interested in basic science, and clinically and laboratory-applicable translational regenerative medicine.
Cardiac Tissue Engineering
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Author : Milica Radisic
language : en
Publisher:
Release Date : 2014
Cardiac Tissue Engineering written by Milica Radisic and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Biotechnology categories.
Annotation Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.
Computer Aided Tissue Engineering
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Author : Alberto Rainer
language : en
Publisher: Humana
Release Date : 2020-07-19
Computer Aided Tissue Engineering written by Alberto Rainer and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-19 with Science categories.
This volume details protocols encompassing different aspects of computer aided design and manufacturing of 3D scaffolds and biofabricated constructs for tissue engineering applications. Chapters are divided into four parts covering optimization of scaffold architectures for computer aided tissue engineering, synthetic routes to biomaterials compatible, technological platforms and manufacturing processes, and relevant applicative scenarios. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computer-Aided Tissue Engineering: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.
Standardisation In Cell And Tissue Engineering
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Author : V Salih
language : en
Publisher: Elsevier
Release Date : 2013-07-31
Standardisation In Cell And Tissue Engineering written by V Salih and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-07-31 with Science categories.
The increased use of biodegradable synthetic or natural scaffolds combined with cells and/or biological molecules, in order to create functional replacement tissue in a damaged tissue site, has led to the need for the development of ‘best practice’ methods in the area of tissue engineering to help ensure the creation of safe, high quality products. Standardisation in cell and tissue engineering introduces concepts and current practice in the field of cell and tissue engineering to a wide audience and aims to provide awareness of the importance of standardisation in this area while suggesting directions for further investigation. Part one provides an overview of methods for cell and tissue engineering and includes chapters on the fundamentals of cell and matrix biology for tissue engineering, 3D collagen biomatrix development, and control and vascularisation of tissue-engineered constructs. Part two begins with a chapter exploring the methods and protocols of standardisation in cell and tissue engineering before moving on to highlight issues of quality control in cell and tissue engineering, standardised chemical analysis and testing of biomaterials and principles of good laboratory practice (GLP) for in vitro cell culture applications. Standardisation in cell and tissue engineering is a standard reference for leading research groups, government agencies, regulatory bodies, and researchers and technicians at all levels across the whole range of disciplines using cell culture within the pharmaceutical, biotechnology and biomedical industries. Introduces concepts and current practice in the field of cell and tissue engineering Highlights the importance of standardisation in cell and tissue engineering and suggests directions for further investigation Explores methods and protocols of standardisation in cell and tissue engineering and issues of quality control in cell and tissue engineering
Cartilage Tissue Engineering
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Author : Pauline M. Doran
language : en
Publisher:
Release Date : 2015
Cartilage Tissue Engineering written by Pauline M. Doran and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with Biotechnology categories.
This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; in vivo testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues.
Methods In Bioengineering
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Author : Francois Berthiaume
language : en
Publisher: Artech House
Release Date : 2010-05
Methods In Bioengineering written by Francois Berthiaume and has been published by Artech House this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-05 with Technology & Engineering categories.
Tissue engineering is an emerging field that involves the combination of materials, cells, and other signals or growth factors to generate new tissue that can be used to repair or replace damaged tissues due to injury or disease. This groundbreaking volume presents the latest methods and protocols for systematically building tissues in 3D configuration outside the body, as well as providing techniques that modulate repair and regeneration processes that occur "in situ" (in their natural or original place).
Organ Regeneration
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Author : Joydeep Basu
language : en
Publisher:
Release Date : 2013
Organ Regeneration written by Joydeep Basu and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with Regenerative medicine categories.
Tissue engineering and regenerative medicine represents a wide array of cell, biomaterial and cell/biomaterial based approaches focusing on the repair, augmentation, and regeneration of diseases tissues and organs. Organ Regeneration: Methods and Protocols has been assembled in response to the growing interest in organ and tissue regeneration as a means to treat disease. Topics cover methods such as isolation and characterization of cells from selected soft tissues and solid organs, preparation and evaluation of natural and synthetic biomaterial scaffolding, implantation of regenerative constructs within experimental animals, and evaluation of regenerative outcomes by molecular and histological methodologies. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Organ Regeneration: Methods and Protocols serves as a detailed guide to aid newcomers and seasoned veterans in their developmental and experimental work in tissue engineering and regenerative medicine.