Trafficking Of Protein Kinase Env7 To The Lysosomal Vacuole Of Saccharomyces Cerevisiae
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Trafficking Of Protein Kinase Env7 To The Lysosomal Vacuole Of Saccharomyces Cerevisiae
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Author : Maria Carmen Ramos (Graduate student)
language : en
Publisher:
Release Date : 2022
Trafficking Of Protein Kinase Env7 To The Lysosomal Vacuole Of Saccharomyces Cerevisiae written by Maria Carmen Ramos (Graduate student) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with Protein kinases categories.
Abstract: The Saccharomyces cerevisiae protein kinase Env7, which negatively regulates homotypic vacuolar membrane fusion during cell stress and budding, has been shown to localize to the yeast vacuole under normal wildtype conditions. Proteins destined for the yeast vacuole traffic either directly from the trans-Golgi network via the AP-3 complex (ALP pathway) or through an endosomal-intermediate (CPY pathway). This study furthers our understanding of Env7 localization to supplement results previously presented in our lab. Here, a time-wise inactivation of the CPY pathway probes at the possibility that Env7 is first mislocalized to or retained at the Golgi. In persistently defective CPY pathway mutants, Env7 protein is further degraded. Single-point mutations of AP-3 complex sorting signal motifs identified within Env7 are deemed unnecessary for the efficient transport to the yeast vacuole. Ultimately, a functional CPY pathway plays an important role in normal Env7 localization at the vacuole, while the ALP pathway does not. Furthermore, an intact CPY pathway is essential for the persistent presence of Env7 in cells, and presumably Env7 stability.
A Study Of Vacuolar Kinase Env7 Trafficking In Saccharomyces Cerevisiae
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Author : Leah Frisardi
language : en
Publisher:
Release Date : 2019
A Study Of Vacuolar Kinase Env7 Trafficking In Saccharomyces Cerevisiae written by Leah Frisardi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with Autophagic vacuoles categories.
Abstract: The vacuole in Saccharomyces cerevisiae (baker’s yeast) is functionally analogous to the human lysosome. Endosome and vacuolar interface 7 (Env7) has been recently identified in the Gharakhanian laboratory as a protein kinase that regulates vacuolar fusion and has a human ortholog, STK16. Previous research in our laboratory has shown that Env7 localizes to the vacuolar membrane in yeast. Proteins destined to function at the yeast vacuole are transported from the Golgi via two distinct pathways: the carboxypeptidase Y (CPY) pathway and the alkaline phosphatase (ALP) pathway. The CPY pathway requires an endosomal intermediate while the ALP pathway requires an adaptor protein complex (AP-3). The CPY pathway serves as the default pathway for the vast majority of proteins moving from the Golgi to the vacuole. Only 9 proteins are known to be transported via the ALP pathway in yeast, all of which are vacuolar membrane proteins and one of which is Yck3, a palmitoylated protein kinase with a similar function of Env7. Proteins normally transported to the yeast vacuole via the ALP pathway are able to partially revert to the CPY pathway when AP-3 function is disrupted. In the absence of a functional CPY pathway, however, the AP-3 pathway remains cargo-specific. Here we show that when the CPY pathway is disrupted in yeast cells, Env7 shows a significant increase in mislocalization. When the ALP pathway is disrupted, Env7 shows no significant mislocalization. Proteins transported via the ALP pathway interact with AP-3 via either an acidic-dileucine-like or a tyrosine-based adaptin sorting signal. Analysis of the amino acid sequence of Env7 showed one potential acidic-dileucine like signal and four potential tyrosine-based adaptin sorting signals. Mutagenesis of two of these sorting signals caused no significant mislocalization of Env7 in yeast. These results implicate the CPY pathway in the vacuolar trafficking of Env7 in yeast.
Studies On Saccharomyces Cerevisiae Vacuolar Membrane Kinase Env7
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Author : Sara Patrice Valencia
language : en
Publisher:
Release Date : 2018
Studies On Saccharomyces Cerevisiae Vacuolar Membrane Kinase Env7 written by Sara Patrice Valencia and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Biological transport categories.
Abstract: The yeast vacuole is a dynamic organelle that is functionally analogous to the mammalian lysosome and serves as a model for the study of membrane fusion and fission. Mechanisms of membrane fission and fusion dynamics have been well conserved from yeast to humans. However, the regulatory mechanisms that govern cellular fission and fusion dynamics remain poorly understood. Our lab has previously established that Env7 is a conserved yeast palmitoylated protein kinase that localizes to the yeast vacuole and negatively regulates vacuole membrane fusion during budding and hyperosmotic stress. Phosphorylation of Env7 is dependent on another vacuolar membrane kinase, Yck3, and is essential to Env7 stability and negative regulation of vacuolar membrane fusion. In this study, we aim to further our understanding of the role Env7 plays at the vacuole by 1) characterizing the phosphorylation of Env7 as a function of cell cycle using cell cycle arrest and synchronization techniques, and 2) generating functional biochemically tagged Yck3 to be used in interaction and phosphorylation assays with Env7. Cell cycle arrest and synchronization techniques have not previously been established in our lab. Here, we report reliable protocols of inducing cell cycle arrest using [alpha]-factor mating pheromone and Hydroxyurea. Results show that Env7 is hyperphosphorylated when cell cycle is arrested at G1 phase using [alpha]-factor mating pheromone. In both cell cycle arrest approaches, vacuoles show significant increase in fragmentation, and Env7 remains localized to the membrane of fragmented vacuoles. In cell culture synchronized with [alpha]-factor, Env7 shows an increase in phosphorylation between S-phase and G2, with decreased phosphorylation in M and G1. We were successful in engineering biochemically tagged Yck3 and established that the expressed 6XHis-Yck3 is functional and able to restore phosphorylation of Env7 in vivo. We also established that overexpressed 6XHis-Yck3 localized correctly to the vacuolar membrane. These tools will be used in future studies on interactions and regulation of membrane fusion.
Env7p Localizes To The Vacuole Membrane And Plays A Role In Vacuolar Protein Processing
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Author : Maribeth Serranilla
language : en
Publisher:
Release Date : 2012
Env7p Localizes To The Vacuole Membrane And Plays A Role In Vacuolar Protein Processing written by Maribeth Serranilla and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Lysosomal storage diseases categories.
Abstract: The vacuole of the yeast Saccharomyces cerevisiae functions comparably to the mammalian lysosome. Its functions include protein degradation, pH/ion homeostasis, down regulation of cell surface receptors, and the ability to adapt to different environmental stresses. Defects in lysosomal trafficking are implicated with neurodegenerative diseases; therefore, understanding vacuolar trafficking will lead to more insight into lysosomal trafficking. Through our genome wide screen to identify mutants defective in the late stages of vacuolar trafficking, our lab has uncovered several previously uncharacterized ENdosome and Vacuole interface (ENV) genes. This study is focused on the characterization of ENV7, a putative serine/threonine kinase that has 29% identity to the human STK16 kinase whose function is unknown. Complementation experiments show that ENV7 is responsible for correct processing of Carboxypeptidase Y. Overexpression of the gene results in no pleiotropic phenotypes within the parameters tested. Env7p localizes at the vacuolar membrane which led to further examination of the deletion mutant, env7[delta], in vacuolar events. Microscopic analyses of env7[delta] reveal no vacuolar morphology, bulk endocytic trafficking, and acidification function defects comparable to WT (BY4742). Growth assays reveal no fitness survival, and pH/ion homeostasis defects associated with env7[delta]. Overall, this study establishes that ENV7 is localized to the vacuolar membrane and is involved in vacuolar protein processing independent of vacuolar morphology and acidification functions.
Saccharomyces Advances In Research And Application 2013 Edition
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Author :
language : en
Publisher: ScholarlyEditions
Release Date : 2013-05-01
Saccharomyces Advances In Research And Application 2013 Edition written by and has been published by ScholarlyEditions this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-05-01 with Science categories.
Saccharomyces—Advances in Research and Application: 2013 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about ZZZAdditional Research in a concise format. The editors have built Saccharomyces—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about ZZZAdditional Research in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Saccharomyces—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
5 Steps To A 5 Ap Biology 2014 2015 Edition
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Author : Mark Anestis
language : en
Publisher: McGraw Hill Professional
Release Date : 2013-07-24
5 Steps To A 5 Ap Biology 2014 2015 Edition written by Mark Anestis and has been published by McGraw Hill Professional this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-07-24 with Science categories.
A PERFECT PLAN for the PERFECT SCORE STEP 1 Set up your study plan with three customized study schedules STEP 2 Determine your readiness with an AP-style diagnostic exam STEP 3 Develop the strategies that will give you the edge on test day STEP 4 Review the terms and concepts you need to score high STEP 5 Build your confidence with full-length practice exams
Report Of The Board Of Governors
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Author : University of Toronto
language : en
Publisher:
Release Date : 1923
Report Of The Board Of Governors written by University of Toronto and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1923 with categories.
Psychosocial Framework Oxford Bibliographies Online Research Guide
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Author : Oxford University Press
language : en
Publisher: Oxford University Press, USA
Release Date : 2010-05-01
Psychosocial Framework Oxford Bibliographies Online Research Guide written by Oxford University Press and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-05-01 with Social Science categories.
This ebook is a selective guide designed to help scholars and students of social work find reliable sources of information by directing them to the best available scholarly materials in whatever form or format they appear from books, chapters, and journal articles to online archives, electronic data sets, and blogs. Written by a leading international authority on the subject, the ebook provides bibliographic information supported by direct recommendations about which sources to consult and editorial commentary to make it clear how the cited sources are interrelated related. A reader will discover, for instance, the most reliable introductions and overviews to the topic, and the most important publications on various areas of scholarly interest within this topic. In social work, as in other disciplines, researchers at all levels are drowning in potentially useful scholarly information, and this guide has been created as a tool for cutting through that material to find the exact source you need. This ebook is a static version of an article from Oxford Bibliographies Online: Social Work, a dynamic, continuously updated, online resource designed to provide authoritative guidance through scholarship and other materials relevant to the study and practice of social work. Oxford Bibliographies Online covers most subject disciplines within the social science and humanities, for more information visit www.aboutobo.com.
Functional Glycomics
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Author : Minoru Fukuda
language : en
Publisher: Academic Press
Release Date : 2010
Functional Glycomics written by Minoru Fukuda and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Medical categories.
In this 3 volume collection focusing on glycomics, readers will appreciate how such discoveries were made and how such methods can be applied for readers' own research efforts Each chapter has been designed so that enough scientific background will be given in each chapter for further development of methods by readers themselves. Useful for all levels of scientists starting from the last years of colleges, graduate students, postdoctoral fellows to professors and to all levels of scientists in research institutes including industry.
Lipid Signaling And Metabolism
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Author : James M. Ntambi
language : en
Publisher: Academic Press
Release Date : 2020-08-09
Lipid Signaling And Metabolism written by James M. Ntambi and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-08-09 with Science categories.
Lipid Signaling and Metabolism provides foundational knowledge and methods to examine lipid metabolism and bioactive lipid signaling mediators that regulate a broad spectrum of biological processes and disease states. Here, world-renowned investigators offer a basic examination of general lipid, metabolism, intracellular lipid storage and utilization that is followed by an in-depth discussion of lipid signaling and metabolism across disease areas, including obesity, diabetes, fatty liver disease, inflammation, cancer, cardiovascular disease and mood-related disorders. Throughout, authors demonstrate how expanding our understanding of lipid mediators in metabolism and signaling enables opportunities for novel therapeutics. Emphasis is placed on bioactive lipid metabolism and research that has been impacted by new technologies and their new potential to transform precision medicine. Provides a clear, up-to-date understanding of lipid signaling and metabolism and the impact of recent technologies critical to advancing new studies Empowers researchers to examine bioactive lipid signaling and metabolism, supporting translation to clinical care and precision medicine Discusses the role of lipid signaling and metabolism in obesity, diabetes, fatty liver disease, inflammation, cancer, cardiovascular disease and mood-related disorders, among others