Type 3 Secretion Systems Methods And Protocols
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Type 3 Secretion Systems Methods And Protocols
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Author : Matthew L. Nilles
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-11-26
Type 3 Secretion Systems Methods And Protocols written by Matthew L. Nilles and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-11-26 with Science categories.
This volume discusses various basic and advanced methods and protocols that have been proven to be successful among certain bacterial species, or a family of species, in type III secretion systems (T3S system). The chapters in this book cover topics such as: site-directed mutagenesis and its application in studying the interactions of T3S components; use of transcriptional control to increase expression and secretion of heterologous proteins in T3S systems; fractionation techniques to examine effector translocation; detecting immune responses to T3S systems; mouse immunization with purified needle proteins from T3S systems and the characterization of the immune responses to these proteins; and detection of protein interactions in T3S systems using yeast 2-hybrid analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and comprehensive, Type 3 Secretion Systems: Methods and Protocols is a valuable resource for anyone interested in learning about the fascinating and ever-changing T3S systems.
Bacterial Secretion Systems
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Author : Laure Journet
language : en
Publisher: Springer Nature
Release Date : 2023-11-06
Bacterial Secretion Systems written by Laure Journet and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-11-06 with Science categories.
This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Secretion Systems: Methods and Protocols- Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
The Bacterial Flagellum
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Author : Tohru Minamino
language : en
Publisher: Humana
Release Date : 2018-06-21
The Bacterial Flagellum written by Tohru Minamino and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-06-21 with Science categories.
This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: Part I describes flagellar type III protein exports, assembly, and gene regulation in S. enterica; Part II explains how to isolate the flagella from the bacterial cell bodies, and further explains how to conduct high-resolution structural and functional analyses of the flagellar motor; Part III talks about how to measure flagellar motor rotation over a wide range of external load, how to measure ion motive force across the cytoplasmic membrane, and how to measure dynamic properties of the flagellar motor proteins by fluorescence microscopy with single molecule precision; and Part IV explores the structure and function of Spirochetal, Vibrio, Shewanella, and Magnetococcus flagellar motors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid in the investigation of the bacterial flagellum resulting from various bacterial species.
Interactions Of Plants With Bacteria And Fungi Molecular And Epigenetic Plasticity Of The Host
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Author : Valentina Fiorilli
language : en
Publisher: Frontiers Media SA
Release Date : 2020-03-31
Interactions Of Plants With Bacteria And Fungi Molecular And Epigenetic Plasticity Of The Host written by Valentina Fiorilli and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-03-31 with categories.
Handbook Of Elispot
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Author : Alexander E. Kalyuzhny
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Handbook Of Elispot written by Alexander E. Kalyuzhny and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.
Molecular Biology Of The Cell
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Author : Bruce Alberts
language : en
Publisher:
Release Date : 2002
Molecular Biology Of The Cell written by Bruce Alberts and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Cytology categories.
Plant Cell Expansion
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Author : José M. Estevez
language : en
Publisher: Humana Press
Release Date : 2014-11-19
Plant Cell Expansion written by José M. Estevez and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-11-19 with Science categories.
This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling and trafficking, and protein and polysaccharides cell wall biosynthesis in real time during cell expansion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cell Expansion: Methods and Protocols is an essential reference book for plant scientist, molecular, and cell biologist as well as plant biochemists.
Pyroptosis
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Author : Susan L. Fink
language : en
Publisher: Springer Nature
Release Date : 2023-04-19
Pyroptosis written by Susan L. Fink and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-04-19 with Science categories.
This volume explores techniques used to study and understand the latest research on pyroptosis. The chapters in this book cover topics such as experimental methods to induce pyroptosis via inflammasomes; approaches to identifying inflammasome activation in humans; ways to induce and detect pore formation; and techniques to evaluate cellular outcomes of pyroptotic cell death. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Pyroptosis: Methods and Protocols is a valuable tool for researchers who want to expand their knowledge of this developing field. It will be especially beneficial for those working in the fields of immunology, cancer biology, microbiology, and cell biology.
Plant Pathogen Interactions
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Author : Pamela C. Ronald
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Plant Pathogen Interactions written by Pamela C. Ronald and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
This book provides key methods, approaches, and strategies to dissect the plant defense response. Addressing methods to identify and characterize plant resistance genes as well as pathogen-associated molecules that trigger the plant defense response, this volume creates a better understanding of the interactions between pathogens and their hosts. This will help to develop better methods for disease control in plants and animals.
Structural And Biochemical Characterization Of The Yaxab Pore Forming Toxin From Yersinia Enterocolitica
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Author : Bastian Bräuning
language : en
Publisher: Springer Nature
Release Date : 2019-09-26
Structural And Biochemical Characterization Of The Yaxab Pore Forming Toxin From Yersinia Enterocolitica written by Bastian Bräuning and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-09-26 with Science categories.
This book discusses the decoding of the lytic mechanism of an α-helical pore-forming toxin, YaxAB, composed of two different subunits. Pore-forming toxins (PFTs) are among the most common bacterial toxins. They are produced by a variety of pathogens, which infect a wide range of organisms including plants, insects and humans. Yet the maturation of these particles and the structural changes required for pore formation are still poorly understood for many PFT families. Using a diverse panel of biochemical and structural techniques, including X-ray crystallography and cryo-electron microscopy, Dr. Bräuning and colleagues have succeeded in identifying the mechanistic contributions of the two toxin components and elucidating the lytic state of the pore complex. The results of this thesis on the YaxAB system are applicable to orthologues from agriculturally relevant insect pathogens, and offer valuable structural and mechanistic insights to inform future bioengineering efforts.