Ultrastructure Techniques For Microorganisms

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Ultrastructure Techniques For Microorganisms

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Author : H.C. Aldrich
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Ultrastructure Techniques For Microorganisms written by H.C. Aldrich and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.

The modem microbiologist is often a real specialist who has difficulty under standing and applying many of the techniques beyond those in his or her own immediate field. On the other hand, most benefits to modem microbiology are obtained when a broad spectrum of scientific approaches can be focused on a problem. In early studies, electron microscopy was pivotal in understanding bacterial and viral morphology, and we still feel that we will understand a disease better if we have seen an electron micrograph of the causative agent. Today, because there is an increased awareness of the need to understand the rela tionships between microbial structure and function, the electron microscope is still one of the most important tools microbiologists can use for detailed analysis of microorganisms. Often, however, the aforementioned modem microbiologist still thinks of ultrastructure as involving negative staining or ultrathin sectioning in order to get a look at the shape of a "bug. " Many of the newer ultrastructure techniques, such as gold-labeled antibody localization, freeze-fracture, X-ray microanalysis, enzyme localization, and even scanning electron microscopy, are poorly under stood by, and therefore forbidding to, the average microbiologist. Even many cell biologists admit to having difficulty staying in touch with current develop ments in the fast-moving field of electron microscopy techniques.

Microscopic Techniques In Biotechnology

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Author : Michael Hoppert
language : en
Publisher: John Wiley & Sons
Release Date : 2006-03-06

Microscopic Techniques In Biotechnology written by Michael Hoppert and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-03-06 with Science categories.

Focusing on all current applications, this book presents the various methods as well as their suitability and limitations for a specific question. One particular highlight is the presentation of all basic information on the structure of the relevant objects, thus allowing readers to choose the most suitable applications for any specific problem. They will also find in-depth background information on structure-function relationships, plus descriptions of sample preparations with respect to a particular technique and the necessary equipment. The whole is rounded off with an overview of the future application potential for devices and applications of upcoming interest in biotechnology.

Manual Of Techniques In Invertebrate Pathology

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Author : Lawrence A. Lacey
language : en
Publisher: Academic Press
Release Date : 2012-06-15

Manual Of Techniques In Invertebrate Pathology written by Lawrence A. Lacey and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-06-15 with Science categories.

1. Initial Handling and Diagnosis of Diseased Invertebrates / Lawrence A. Lacey and Leellen Solter -- 2. Basic Techniques in Insect Virology / Karolin E. Eberle, Jorg T. Wennmann, Regina G. Kleespies and Johannes A. Jehle -- 3. Isolation, Culture, Preservation, and Identification of Entomopathogenic Bacteria of the Bacilli. / Tanja W. Fisher and Steven F. Garczynski -- 4. Bioassay of Bacterial Entomopathogens Against Insect Larvae / Maureen O'Callaghan, Travis R. Glare and Lawrence A. Lacey --5. Bacteria for use Against Soil-Inhabitating Insects / Albrecht M. Koppenhofer, Trevor A. Jackson, and Michael G. Klein -- 6. Identification of Entomopathogenic Fungi / Richard A. Humber -- 7. Laboratory Techniques Used for Entomopathogenic Fungi: Hypocreales / G. Douglas Inglis, Juerg Enkerli, and Mark S. Goettel -- 8. Mass Production of Entomopathogenic Hypocreales / Stefan T. Jaronski and Mark A. Jackson -- 9. Methods for the Study of Entomophthorales / Ann E. Hajek, Bernard Papierok, and Jurg ...

Ghadially S Ultrastructural Pathology Of The Cell And Matrix

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Author : Bart Wagner
language : en
Publisher: CRC Press
Release Date : 2025-07-30

Ghadially S Ultrastructural Pathology Of The Cell And Matrix written by Bart Wagner and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-07-30 with Medical categories.

This established handbook is unique in illustrating and reviewing the cell and extracellular matrix, organelle by organelle (with numerous subsections) with emphasis on human pathology. Anyone studying normal or pathological tissues whether human, animal, or tissue culture, by conventional transmission electron microscopy for diagnosis or research, will use this book to understand what it is they are examining—without having to go through large volumes of previously published articles. Key Features: Illustrates cell ultrastructure with electron micrographs Reviews extracellular matrix structure Assists pathologists in diagnosis of cellular pathologies Includes contributions from an international team of leading researchers

Structure Of Phototrophic Prokaryotes

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Author : John F. Stolz
language : en
Publisher: CRC Press
Release Date : 1990-11-27

Structure Of Phototrophic Prokaryotes written by John F. Stolz and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-11-27 with Science categories.

This book provides an in-depth review of the ultrastructure of the phototrophic bacteria and how electron microscopy is used in the study of light-harvesting antennae and reactions centers, as well as ecology. Topics discussed include detailed methods and models for the three basic types of photosynthetic apparatus. The thylakoid (cyanobacteria), intracytoplasmic chromatophore membrane (purple bacteria), and chlorosome (green bacteria) models are presented based on ultrastructural studies including ultra-thin sections, 3-D reconstruction of thick sections, freeze-fracture replicates, and immunolabeling. The book also explains how species of phototrophic bacteria can be identified in natural populations by their ultrastructure. Structure of Phototrophic Prokaryotes will be an invaluable reference resource for researchers and students working in photosynthesis and microbial ecology.

Methods For General And Molecular Microbiology

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Author : C. A. Reddy
language : en
Publisher: American Society for Microbiology Press
Release Date : 2007-08-17

Methods For General And Molecular Microbiology written by C. A. Reddy and has been published by American Society for Microbiology Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-17 with Science categories.

A first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. • Provides a comprehensive compendium of methods used in general and molecular microbiology. • Contains many new and expanded chapters, including a section on the newly important field of community and genomic analysis. • Provides step-by-step coverage of procedures, with an extensive list of references to guide the user to the original literature for more complete descriptions. • Presents methods for bacteria, archaea, and for the first time a section on mycology. • Numerous schematics and illustrations (both color and black and white) help the reader to easily understand the topics presented.

Manual Of Techniques In Insect Pathology

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Author : Lawrence A. Lacey
language : en
Publisher: Academic Press
Release Date : 1997-02-27

Manual Of Techniques In Insect Pathology written by Lawrence A. Lacey and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-02-27 with Technology & Engineering categories.

Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advances researches and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.This manual describes the broad array of techniques that are used in insect pathology. It will provide biologists, insect pathologists, entomologists, and those interested in biological control, with the necessary information to work on a variety of pathogen groups.This book will be an essential laboratory reference for insect pathologists.Features include:* Step by-step instructions on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens* Details of the practical knowledge needed by beginners to apply the techniques* Chapters written by an international group of experts* Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques* Provides extensive supplemental literature and recipes for media, fixatives and stains

Histology Ultrastructure And Molecular Cytology Of Plant Microorganism Interactions

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Author : Michel Nicole
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Histology Ultrastructure And Molecular Cytology Of Plant Microorganism Interactions written by Michel Nicole and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

Plants interact with a large number of microoganisms which have a major impact on their growth either by establishing mutually beneficial symbiotic relationships or by developing as pathogens at the expense of the plant with deleterious effects. These microorganisms differ greatly not only in their nature (viruses, phytoplasmas, bacteria, fungi, nematodes, ... ) but also in the way they contact, penetrate and invade their host. Histology and cytology have brought an essential contribution to our knowledge of these phenomena. They have told us for instance, how specialized structures of the pathogen are often involved in the adhesion and penetration into the plant, how the interface between both organisms is finely arranged at the cellular level, or what structural alterations affect the infected tissues. They have thus set the stage for the investigations of the underlying molecular mechanisms could be undertaken. Such investigations have been remarkably successful in the recent years, expanding considerably our understanding of plant-microorganism interactions in terms of biochemical changes, rapid modifications of enzymatic activities, coordinated gene activation, signal reception and transduction. Biochemistry, molecular biology and cellular physiology have taken precedence in the phytopathologist's set of methods.

Molecular Methods In Plant Pathology

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Author : U. S. Singh
language : en
Publisher: CRC Press
Release Date : 2017-12-14

Molecular Methods In Plant Pathology written by U. S. Singh and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-14 with Science categories.

Molecular Methods in Plant Pathology covers methods in phytopathology at the molecular level, including PCR techniques, electron microscopy, tissue culturing, and the cloning of disease-resistant genes. Phytopathologists, botanists, horticulturists, and anyone working in agriculture will find this a useful reference on biophysical, biochemical, biomolecular, and biotechnological methods.

Biological Electron Microscopy

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Author : Michael J. Dykstra
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-06-28

Biological Electron Microscopy written by Michael J. Dykstra and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-28 with Science categories.

Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.