Ultrastructure Techniques For Microorganisms
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Ultrastructure Techniques For Microorganisms
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Author : H.C. Aldrich
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Ultrastructure Techniques For Microorganisms written by H.C. Aldrich and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
The modem microbiologist is often a real specialist who has difficulty under standing and applying many of the techniques beyond those in his or her own immediate field. On the other hand, most benefits to modem microbiology are obtained when a broad spectrum of scientific approaches can be focused on a problem. In early studies, electron microscopy was pivotal in understanding bacterial and viral morphology, and we still feel that we will understand a disease better if we have seen an electron micrograph of the causative agent. Today, because there is an increased awareness of the need to understand the rela tionships between microbial structure and function, the electron microscope is still one of the most important tools microbiologists can use for detailed analysis of microorganisms. Often, however, the aforementioned modem microbiologist still thinks of ultrastructure as involving negative staining or ultrathin sectioning in order to get a look at the shape of a "bug. " Many of the newer ultrastructure techniques, such as gold-labeled antibody localization, freeze-fracture, X-ray microanalysis, enzyme localization, and even scanning electron microscopy, are poorly under stood by, and therefore forbidding to, the average microbiologist. Even many cell biologists admit to having difficulty staying in touch with current develop ments in the fast-moving field of electron microscopy techniques.
Microbial Ultrastructure
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Author : Society for Applied Bacteriology
language : en
Publisher:
Release Date : 1976
Microbial Ultrastructure written by Society for Applied Bacteriology and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1976 with Science categories.
Structure And Ultrastructure Of Microorganisms
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Author : Ernst Max Brieger
language : en
Publisher:
Release Date : 1963
Structure And Ultrastructure Of Microorganisms written by Ernst Max Brieger and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1963 with Bacteria categories.
Structure And Ultrastructure Of Microorganisms
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Author : Ernest Max Brieger
language : en
Publisher:
Release Date : 1963
Structure And Ultrastructure Of Microorganisms written by Ernest Max Brieger and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1963 with categories.
Electron Microscopy In Microbiology
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Author :
language : en
Publisher: Academic Press
Release Date : 1988-10-01
Electron Microscopy In Microbiology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988-10-01 with Science categories.
This volume of this acclaimed series deals with electron microscopic techniques applied for the elucidation of microbial structures and structure-function relationships at cellular, sub-cellular, and macromolecular levels. Many of the recent findings on ultrastructural features of microorganisms have been obtained with newly developed methods, though classical approaches have not lost their validity. Therefore, both conventional and new methods have been incorporated into this volume. The topics dealt with are meaningful not only in bacterial cytology but also in physiology, enzymology, biochemistry, and molecular biology, and include aspects of medical and biotechnological application.
Hydrocarbon And Lipid Microbiology Protocols
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Author : Terry J. McGenity
language : en
Publisher: Springer
Release Date : 2016-04-22
Hydrocarbon And Lipid Microbiology Protocols written by Terry J. McGenity and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-22 with Science categories.
This Volume presents key microscopy and imaging methods for revealing the structure and ultrastructure of environmental and experimental samples, of microbial communities and cultures, and of individual cells. Method adaptations that specifically address problems concerning the hydrophobic components of samples are highlighted and discussed. The methods described range from electron microscopy and light and fluorescence microscopy, to confocal laser-scanning microscopy, and include experimental set-ups for the analysis of interfacial processes like microbial growth and activities at hydrocarbon:water interfaces, biofilms and microbe:mineral interfaces. Three forms of fluorescence in situ hybridization - CARD-FISH, MAR-FISH and Two-pass TSA-FISH - are described for the ecophysiological analysis of functionally active microbes in samples. The methods presented will enable readers to obtain an ultrastructural picture of, and identify the key functional microbes in, samples under investigation. This in turn will constitute a key framework for the interpretation of information from other experimental approaches, such as physicochemical analyses and genomic investigations. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.
Structure And Ultrastructure Of Microorganisms
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Author : E. M. Brieger
language : en
Publisher: Academic Press
Release Date : 1963
Structure And Ultrastructure Of Microorganisms written by E. M. Brieger and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1963 with Cytology categories.
Structure and Ultrastructure of Microorganisms ...
Histology Ultrastructure And Molecular Cytology Of Plant Microorganism Interactions
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Author : Michel Nicole
language : en
Publisher: Springer Science & Business Media
Release Date : 1996-02-29
Histology Ultrastructure And Molecular Cytology Of Plant Microorganism Interactions written by Michel Nicole and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-02-29 with Science categories.
This book provides comprehensive, up-to-date descriptions of cellular aspects of interactions between plants and microorganisms, including lichens, and also covers infections by viruses, trypanosomes and nematodes. The detailed reviews are accompanied by nearly 250 appropriate micrographs. Topics covered include: techniques that may be of use in studying plant infections such as colloidal gold cytochemistry, in situ hybridization, histochemical detection of polyphenols, cytodetection of pathogens, monoclonal antibodies and image analysis; host aggression by bacteria, fungi and nematodes with emphasis on adhesion, penetration and plant cell modifications; defense mechanisms of infected plants, including trees, cytolocalization of molecules involved in host resistance, plant responses to parasites or symbiotic microorganisms. Comparisons are also given between pathogenic interactions and mutualistic associations. Audience: An invaluable source of information and references for research scientists and students interested not only in plant-microbe interactions but also in cell biology in general.
Ultrastructure Of Bacterial Viruses
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Author : Anna S. Tikhonenko
language : en
Publisher: Springer
Release Date : 1970
Ultrastructure Of Bacterial Viruses written by Anna S. Tikhonenko and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 1970 with Medical categories.
Mter the discoveryof the tobacco mosaic virus by D. I. Ivanov skU in 1892 [14], the new science of virology was born and began to develop rapidly. The number of viruses now known is enormous and they can infect nearly all animal and plant organisms. Microorganisms themselves are no exception to this rule. Despite intensive study of Vlruses, their origin and nature are still a subject for speculation and hypothesis. The general concept of viruses embraces a wide group of biologically active structures occupying an intermediate position between living and nonliving matter. The dual character of viruses is determined by the fact that, while they do not possess an inde pendent system of metabolism, which is a characteristic feature of every living being, they nevertheless carry within themselves all the necessary information for autoreproduction. A striking feature of the virus is that it consists essentially of two components: a protein envelope and the nucleic acid con tained within it. In contrast to the elementary structural unit of the living or ganism, the cell, which contains two types of nucleic acid (DNA and RNA), the virus particle contains only one type of nucleic acid - either DNA or RNA. It is perhaps this which is responsible for the imperfection of the virus as a living organism.
Manual Of Techniques In Invertebrate Pathology
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Author : Lawrence A. Lacey
language : en
Publisher: Academic Press
Release Date : 2012-04-25
Manual Of Techniques In Invertebrate Pathology written by Lawrence A. Lacey and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-04-25 with Science categories.
Manual of Techniques in Invertebrate Pathology, Second Edition, describes a wide range of techniques used in the identification, isolation, propagation/cultivation, bioassay, quantification, preservation, and storage of the major groups of entomopathogens, including entomophthorales, entomopathogenic fungi, entomopathogenic bacteria of the Bacilli, Nematode parasites, and pathogens and parasites of terrestrial molluscs. The book presents the perspectives of an international group of experts in the fields of invertebrate pathology, including microbiology, mycology, virology, nematology, biological control, and integrated pest management. Organized into 15 chapters, the book covers methods for the study of virtually every major group of entomopathogen, as well as methods for discovery and diagnosis of entomopathogens and the use of complementary methods for microscopy. It discusses the use of molecular techniques for identifying and determining phylogeny, factors that contribute to resistance to entomopathogens, and several other aspects of the science of invertebrate pathology. It also explains initial handling and diagnosis of diseased invertebrates, basic techniques in insect virology, and bioassay of bacterial entomopathogens against insect larvae. In addition, the reader is introduced to the use of bacteria against soil-inhabiting insects and preservation of entomopathogenic fungal cultures. The remaining chapters focus on research methods for entomopathogenic microsporidia and other protists, how the pathogenicity and infectivity of entomopathogens to mammals are tested, and preparations of entomopathogens and diseased specimens for more detailed study using microscopy. Experienced insect pathologists, biologists, entomologists, students, biotechnology personnel, technicians, those working in the biopesticide industry, and government regulators will find this manual extremely helpful. Step-by-step instructions for the latest techniques on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens New edition fully updated to address changes in the taxonomy of the vast majority of taxa Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques Provides extensive supplemental literature and recipes for media, fixatives and stains