Whole Cell Redox Biocatalysis Driven By Photosynthesis
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Whole Cell Redox Biocatalysis Driven By Photosynthesis
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Author : Anna Hoschek
language : en
Publisher:
Release Date : 2019
Whole Cell Redox Biocatalysis Driven By Photosynthesis written by Anna Hoschek and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with categories.
Photosynthesis
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Author : Matthias Rögner
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2021-10-25
Photosynthesis written by Matthias Rögner and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-10-25 with Science categories.
This book assembles state-of-the-art approaches for harnessing light energy as a model to develop natural systems such as biofuels. After the basics and potential of photosynthesis of microalgae it discusses topics from engineering micro-algae towards increased photosynthetic efficiency till the optimization of photobioreactor techniques for enhanced biotechnological applications such as cyanobacteria.
Systems Biocatalysis For Bioprocess Design
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Author : Roland Wohlgemuth
language : en
Publisher: Frontiers Media SA
Release Date : 2022-12-02
Systems Biocatalysis For Bioprocess Design written by Roland Wohlgemuth and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-12-02 with Science categories.
Production Of Biofuels And Chemicals From Sustainable Recycling Of Organic Solid Waste
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Author : Zhen Fang
language : en
Publisher: Springer Nature
Release Date : 2022-05-18
Production Of Biofuels And Chemicals From Sustainable Recycling Of Organic Solid Waste written by Zhen Fang and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-05-18 with Science categories.
This book covers sustainable recycling processes (e.g. physical, biological, chemical, and thermo-chemical) of multiple organic solid wastes, provides methods for material recycle of wastes into value-added products including fuels and commodity chemicals that are able to be directly applied to promote manufacturing processes. Aimed at improving the awareness of effective conversion protocols and for developing innovative biomass conversion processes, this text was conceived as a collection of studies on state-of-art techniques and know-how for production of biofuels and chemicals from sustainable recycling of organic solid wastes. Topics in the text are discussed in terms of addressing recent advances, assessing and highlighting promising new methods or new technological strategies and direct conversion of organic solid wastes to process feeds. Highly-recognized authorities, experts and professionals have contributed individual chapters in selected areas to cover the overall topic in a comprehensive manner.
Asymmetric And Selective Biocatalysis
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Author : Jose M. Palomo
language : en
Publisher: MDPI
Release Date : 2019-04-12
Asymmetric And Selective Biocatalysis written by Jose M. Palomo and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-12 with Science categories.
This Issue contains one communication, six articles, and two reviews. The communication from Paola Vitale et al. represents a work where whole cells were used as biocatalysts for the reduction of optically active chloroalkyl arylketones followed by a chemical cyclization to give the desired heterocycles. Among the various whole cells screened (baker’s yeast, Kluyveromyces marxianus CBS 6556, Saccharomyces cerevisiae CBS 7336, Lactobacillus reuteri DSM 20016), baker’s yeast provided the best yields and the highest enantiomeric ratios (95:5) in the bioreduction of the above ketones. In this respect, valuable chiral non-racemic functionalized oxygen-containing heterocycles (e.g., (S)-styrene oxide, (S)-2-phenyloxetane, (S)-2-phenyltetrahydrofuran), amenable to be further elaborated on, can be smoothly and successfully generated from their prochiral precursors. Studies about pure biocatalysts with mechanistical studies, application in different reactions, and new immobilization methods for improving their stability were reported in five different articles. The article by Su-Yan Wang et al. describes the cloning, expression, purification, and characterization of an N-acetylglucosamine 2-epimerase from Pedobacter heparinus (PhGn2E). For this, several N-acylated glucosamine derivatives were chemically synthesized and used to test the substrate specificity of the enzyme. The mechanism of the enzyme was studied by hydrogen/deuterium NMR. The study at the anomeric hydroxyl group and C-2 position of the substrate in the reaction mixture confirmed the epimerization reaction via ring-opening/enolate formation. Site-directed mutagenesis was also used to confirm the proposed mechanism of this interesting enzyme. The article by Forest H. Andrews et al. studies two enzymes, benzoylformate decarboxylase (BFDC) and pyruvate decarboxylase (PDC), which catalyze the non-oxidative decarboxylation of 2-keto acids with different specificity. BFDC from Pseudomonas putida exhibited very limited activity with pyruvate, whereas the PDCs from S. cerevisiae or from Zymomonas mobilis showed virtually no activity with benzoylformate (phenylglyoxylate). After studies using saturation mutagenesis, the BFDC T377L/A460Y variant was obtained, with 10,000-fold increase in pyruvate/benzoylformate. The change was attributed to an improvement in the Km value for pyruvate and a decrease in the kcat value for benzoylformate. The characterization of the new catalyst was performed, providing context for the observed changes in the specificity. The article by Xin Wang et al. compares two types of biocatalysts to produce D-lysine L-lysine in a cascade process catalyzed by two enzymes: racemase from microorganisms that racemize L-lysine to give D,L-lysine and decarboxylase that can be in cells, permeabilized cells, and the isolated enzyme. The comparison between the different forms demonstrated that the isolated enzyme showed the higher decarboxylase activity. Under optimal conditions, 750.7 mmol/L D-lysine was finally obtained from 1710 mmol/L L-lysine after 1 h of racemization reaction and 0.5 h of decarboxylation reaction. D-lysine yield could reach 48.8% with enantiomeric excess (ee) of 99%. In the article by Rivero and Palomo, lipase from Candida rugosa (CRL) was highly stabilized at alkaline pH in the presence of PEG, which permitted its immobilization for the first time by multipoint covalent attachment on different aldehyde-activated matrices. Different covalent immobilized preparation of the enzyme was successfully obtained. The thermal and solvent stability was highly increased by this treatment, and the novel catalysts showed high regioselectivity in the deprotection of per-O-acetylated nucleosides. The article by Robson Carlos Alnoch et al. describes the protocol and use of a new generation of tailor-made bifunctional supports activated with alkyl groups that allow the immobilization of proteins through the most hydrophobic region of the protein surface and aldehyde groups that allows the covalent immobilization of the previously adsorbed proteins. These supports were especially used in the case of lipase immobilization. The immobilization of a new metagenomic lipase (LipC12) yielded a biocatalyst 3.5-fold more active and 5000-fold more stable than the soluble enzyme. The PEGylated immobilized lipase showed high regioselectivity, producing high yields of the C-3 monodeacetylated product at pH 5.0 and 4 °C. Hybrid catalysts composed of an enzyme and metallic complex are also treated in this Special Issue. The article by Christian Herrero et al. describes the development of the Mn(TpCPP)-Xln10A artificial metalloenzyme, obtained by non-covalent insertion of Mn(III)-meso-tetrakis(p-carboxyphenyl)porphyrin [Mn(TpCPP), 1-Mn] into xylanase 10A from Streptomyces lividans (Xln10A). The complex was found able to catalyze the selective photo-induced oxidation of organic substrates in the presence of [RuII(bpy)3]2+ as a photosensitizer and [CoIII(NH3)5Cl]2+ as a sacrificial electron acceptor, using water as oxygen atom source. The two published reviews describe different subjects with interest in the fields of biocatalysis and mix metallic-biocatalysis, respectively. The review by Anika Scholtissek et al. describes the state-of-the-art regarding ene-reductases from the old yellow enzyme family (OYEs) to catalyze the asymmetric hydrogenation of activated alkenes to produce chiral products with industrial interest. The dependence of OYEs on pyridine nucleotide coenzyme can be avoided by using nicotinamide coenzyme mimetics. In the review, three main classes of OYEs are described and characterized. The review by Yajie Wang and Huimin Zhao highlights some of the recent examples in the past three years that combine transition metal catalysis with enzymatic catalysis. With recent advances in protein engineering, catalyst synthesis, artificial metalloenzymes, and supramolecular assembly, there is great potential to develop more sophisticated tandem chemoenzymatic processes for the synthesis of structurally complex chemicals. In conclusion, these nine publications give an overview of the possibilities of different catalysts, both traditional biocatalysts and hybrids with metals or organometallic complexes to be used in different processes—particularly in synthetic reactions—under very mild reaction conditions.
Bioprocess Intensification
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Author : Dirk Holtmann
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2024-07-01
Bioprocess Intensification written by Dirk Holtmann and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-07-01 with Science categories.
Application of Process Intensification (PI) presents a set of radically innovative principles in process and equipment design, which can bring significant benefits in terms of process efficiency, capital and operating expenses, quality, process safety, and sustainability. Typical approaches in bioprocess intensification are the reduction of the number of production steps, continuous processing, integrated processes, and alternative energy inputs.
Advances In Green Synthesis
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Author : Inamuddin
language : en
Publisher: Springer Nature
Release Date : 2021-05-18
Advances In Green Synthesis written by Inamuddin and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-05-18 with Science categories.
This edited book focusses on green chemistry as the research community endeavours to create eco-friendly materials and technologies. It provides an in-depth overview of the fundamentals, key concepts and experimental techniques for eco-friendly synthesis of organic compounds and metal/metal oxide nanoparticles/nanomaterials. It also emphasizes the mechanisms, designing and industrial technologies for green synthesis and its applications. Each chapter brings the recent developments, state of the art, challenges and perspectives which cover all the aspects in one place, and which concern the green synthesis and evolution. Authored by world-renowned experts in a broad range of green chemistry sectors, this book is an archival reference guide for researchers, engineers, scientists and postgraduates working in the field of sustainable science, green chemistry, environmental science, engineering sciences and industrial technologies.
Recent Advances In Biocatalysis And Metabolic Engineering For Biomanufacturing
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Author : Eun Yeol Lee
language : en
Publisher: MDPI
Release Date : 2019-09-23
Recent Advances In Biocatalysis And Metabolic Engineering For Biomanufacturing written by Eun Yeol Lee and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-09-23 with Science categories.
The use of biocatalysts, including enzymes and metabolically engineered cells, has attracted a great deal of attention in the chemical and bio-industry, because biocatalytic reactions can be conducted under environmentally-benign conditions and in more sustainable ways. The catalytic efficiency and chemo-, regio-, and stereo-selectivity of enzymes can be enhanced and modulated using protein engineering. Metabolic engineering seeks to enhance cellular biosynthetic productivity of target metabolites via controlling and redesigning metabolic pathways using multi-omics analysis, genome-scale modeling, metabolic flux control, and reconstruction of novel pathways. The aim of this book is to cover the recent advances in biocatalysis and metabolic engineering for biomanufacturing of biofuels, chemicals, biomaterials, and pharmaceuticals. Reviews and original research articles on the development of new strategies to improve the catalytic efficiency of enzymes, biosynthetic capability of cell factories, and their applications in production of various bioproducts and chemicals are included.
The Autotrophic Biorefinery
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Author : Robert Kourist
language : en
Publisher: Walter de Gruyter GmbH & Co KG
Release Date : 2021-10-25
The Autotrophic Biorefinery written by Robert Kourist and has been published by Walter de Gruyter GmbH & Co KG this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-10-25 with Science categories.
The depletion of fossil resources and an ever-growing human population create an increasing demand for the development of sustainable processes for the utilization of renewable resources. As autotrophic microorganisms offer numerous metabolic pathways for the fixation of carbon dioxide and the metabolic utilization of light, electricity and inorganic energy donors, they are expected to play a pivotal role in an emerging carbon neutral society. This text-book presents the metabolic principles of autotrophy and current efforts for their utilization in biotechnology, including photoautotrophic, chemolithoautotrophic and electroautotrophic organisms. It outlines how modern molecular biology and process engineering create technologies that allow to use industrial off-gases and inorganic energy for the synthesis of bio-based plastics, materials and other chemical products. The text-book is ideally suited for students in advanced graduate and master courses and offers a reference for PhD students, engineers, chemists, biologists and all with an interests in biotechnology and renewable resources.
Biophotoelectrochemistry From Bioelectrochemistry To Biophotovoltaics
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Author : Lars J.C. Jeuken
language : en
Publisher: Springer
Release Date : 2017-05-22
Biophotoelectrochemistry From Bioelectrochemistry To Biophotovoltaics written by Lars J.C. Jeuken and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-05-22 with Science categories.
This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.