Yeast Protocols
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Yeast Protocols
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Author : Wei Xiao
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Yeast Protocols written by Wei Xiao and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.
Yeast Genetics
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Author : Jeffrey S. Smith
language : en
Publisher: Humana Press
Release Date : 2014-09-12
Yeast Genetics written by Jeffrey S. Smith and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-09-12 with Science categories.
Yeast Genetics: Methods and Protocols is a collection of methods to best study and manipulate Saccharomyces cerevisiae, a truly genetic powerhouse. The simple nature of a single cell eukaryotic organism, the relative ease of manipulating its genome and the ability to interchangeably exist in both haploid and diploid states have always made it an attractive model organism. Genes can be deleted, mutated, engineered and tagged at will. Saccharomyces cerevisiae has played a major role in the elucidation of multiple conserved cellular processes including MAP kinase signaling, splicing, transcription and many others. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Yeast Genetics: Methods and Protocols will provide a balanced blend of classic and more modern genetic methods relevant to a wide range of research areas and should be widely used as a reference in yeast labs.
Food Microbiology Protocols
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Author : John F. T. Spencer
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Food Microbiology Protocols written by John F. T. Spencer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
Two of the recent books in the Methods in Molecular Biology series, Yeast Protocols and Pichia Protocols, have been narrowly focused on yeasts and, in the latter case, particular species of yeasts. Food Microbiology Pro- cols, of necessity, covers a very wide range of microorganisms. Our book treats four categories of microorganisms affecting foods: (1) Spoilage organisms; (2) pathogens; (3) microorganisms in fermented foods; and (4) microorganisms p- ducing metabolites that affect the flavor or nutritive value of foods. Detailed information is given on each of these categories. There are several chapters devoted to the microorganisms associated with fermented foods: these are of increasing importance in food microbiology, and include one bacteriophage that kills the lactic acid bacteria involved in the manufacture of different foods—cottage cheese, yogurt, sauerkraut, and many others. The other nine chapters give procedures for the maintenance of lactic acid bacteria, the isolation of plasmid and genomic DNA from species of Lac- bacillus, determination of the proteolytic activity of lactic acid bacteria, det- mination of bacteriocins, and other important topics.
Pichia Protocols
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Author : James M Cregg
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-08-08
Pichia Protocols written by James M Cregg and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-08 with Science categories.
This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.
Recombinant Protein Production In Yeast
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Author : Roslyn M. Bill
language : en
Publisher: Humana Press
Release Date : 2016-05-01
Recombinant Protein Production In Yeast written by Roslyn M. Bill and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-01 with Medical categories.
This book reviews preparation of expression vectors, generation of high-yielding clones, scale-up, disruption of yeast cells to enable isolation of recombinant protein prior to purification and more, in the popular Methods in Molecular Biology format."
Methods In Yeast Genetics
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Author : David C. Amberg
language : en
Publisher: CSHL Press
Release Date : 2005
Methods In Yeast Genetics written by David C. Amberg and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Genetics categories.
"Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
As For Protocols
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Author : Re'al Christian
language : en
Publisher: Amherst College Press
Release Date : 2025-03-04
As For Protocols written by Re'al Christian and has been published by Amherst College Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-03-04 with Art categories.
Explicitly--or not--protocols determine much of what we do. Far exceeding traditional notions of "good manners," protocols are systems of language that regulate how we relate to each other, to our cultural, social, and political environments, and to the technologies that create them. The first publication to look at protocols across a wide range of disciplines, As for Protocols brings together contributions by twenty-two international artists, writers, scholars, musicians, architects, and scientists who explore protocols across various fields, foregrounding opportunities for creating new protocols that are inclusive and equitable. Through essays, artworks, interviews, and scores, the book speaks to protocols as practice--neither conventional mannerisms nor abstract concepts, but material processes, relational affinities, shared responsibilities, and mutual care. Edited by Re'al Christian, Carin Kuoni, and Eriola Pira, an opening score by Raven Chacon and contributions by Salome Asega, Carolina Caycedo with Lupita Limón Corrales, Raven Chacon, Jesse Chun, Asia Dorsey, Taraneh Fazeli and Cannach MacBride, Pablo Helguera, Emmanuel Iduma, Mary Maggic, Shannon Mattern, V. Mitch McEwen with Nadir Jeevanjee, Rashaun Mitchell with Silas Riener, Romy Opperman, Rasheedah Phillips, Leanne Betasamosake Simpson with Maria Hupfield, Ultra-red with Robert Sember, and Underground Resistance.
Laboratory Protocols In Fungal Biology
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Author : Vijai Kumar Gupta
language : en
Publisher: Springer Nature
Release Date : 2022-02-03
Laboratory Protocols In Fungal Biology written by Vijai Kumar Gupta and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-02-03 with Science categories.
Mycology has an integral role to play in the development of the biotechnology and biomedical sectors. It has become a subject of increasing importance as new fungi and their associated biomolecules are identified. As this discipline comes to the forefront of research in these sectors, the requirement for a consolidation of available research approaches is required. The First Edition of this book has a few basic and applied protocols. With the Second Edition, this book provides consolidated information on recent developments and the most widely used mycological methods available in the fields of biochemistry, biotechnology and microbiology. The methods outlined offer clear and concise directions to the reader and covers both standard protocols and more applied mycological methods. This book provides useful information for undergraduates, post-graduates, and specialists and researchers studying fungal biology.
Developmental Biology Protocols
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Author : Rocky S. Tuan
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Developmental Biology Protocols written by Rocky S. Tuan and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.
Yeast Physiology And Biotechnology
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Author : Graeme M. Walker
language : en
Publisher: John Wiley & Sons
Release Date : 1998-04-08
Yeast Physiology And Biotechnology written by Graeme M. Walker and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-04-08 with Science categories.
Yeasts are the world's premier industrial micro-organisms. In addition to their wide exploitation in the production of foods, beverages and pharmaceuticals, yeasts also play significant roles as model eukaryotic cells in furthering our knowledge in the biological and biomedical sciences. In order for modern biotechnology to fully exploit the activities of yeasts, it is essential to appreciate aspects of yeast cell physiology. In recent years, however, our knowledge of yeast physiological phenomena has lagged behind that of yeast genetics and molecular biology. Yeast Physiology and Biotechnology redresses the balance by linking key aspects of yeast physiology with yeast biotechnology. Individual chapters provide broad and timely coverage of yeast cytology, nutrition, growth and metabolism - important aspects of yeast cell physiology which are pertinent to the practical uses of yeasts in industry. The final chapter reviews traditional, modern and emerging biotechnologies in which roles of yeasts in the production of industrial commodities and their value in biomedical research are fully discussed. Relevant aspects of classical and modern yeast genetics and molecular biology are fully integrated into the appropriate chapters. This up-to-date and fully referenced book is aimed at advanced undergraduate and postgraduate bioscience students,but will also prove to be a valuable source of information for yeast researchers and technologists.