Zymography
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Zymography
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Author : Raouf A. Khalil
language : en
Publisher: Springer Nature
Release Date : 2025-04-22
Zymography written by Raouf A. Khalil and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-04-22 with Science categories.
Volume 2 describes how to determine the activity of different isozymes, allozymes, and families of proteinases to advance the fields of enzymology and molecular evolution, and provides useful biomarkers for various biological processes, pathological conditions, and clinical disorders. The chapters in Volume 2 are organized in three parts. Part I introduces in situ zymography and localization of bright green-fluorescent gelatinase activity in tissue sections, in situ zymography in formalin-fixed paraffin-embedded and mineralized tissues, and in vivo zymography as an essential activity assay for studying the activity of matrix metalloproteinases (MMPs) in a cell-specific manner in the brain. Part II focuses on biological applications of zymography such as fundamentals of zymography and its applications to the study of biological samples, gelatin zymography to quantify MMP-2 and MMP-9 in complex biological specimens, and detection of proteolytic enzymes in polyacrylamide gels supplemented with diverse biological substrates. Part III focuses on potential clinical applications of zymography, with chapters describing assessment of MMP-2 and MMP-9 hydrolytic activity in preclinical and clinical tissue samples, the use of zymography to assess circulating MMP-2 and MMP-9 in plasma and serum and in pathological conditions, and the use of zymography for the detection of bacterial proteases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Zymography: Biological and Clinical Applications, Volume II is a valuable resource for both experts in the field, as well as new scientists aspiring to learn and perform successful zymography techniques.!-- [if !supportLineBreakNewLine]--!--[endif]--
Epidermal Cells
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Author : Kursad Turksen
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Epidermal Cells written by Kursad Turksen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Medical categories.
A collection of cutting-edge methods to analyze and manipulate epidermal cellprecursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, and for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. These readily reproducible techniques broaden our understanding of the biology of epidermal cells and of their utility in normal tissue homeostasis and regenerative medicine applications.
Intracranial Pressure And Brain Monitoring Xiii
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Author : Geoffrey Manley
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-05-06
Intracranial Pressure And Brain Monitoring Xiii written by Geoffrey Manley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-05-06 with Medical categories.
86 short papers originating from the 13th International Symposium on Intracranial Pressure and Brain Monitoring held in July 2007 in San Francisco present experimental as well as clinical research data on invasive and non-invasive intracranial pressure and brain biochemistry monitoring. The papers have undergone a peer-reviewing and are organized in eight sections: brain injury: ICP management and cerebral physiology; hydrocephalus and cerebrospinal fluid dynamics; advanced neuromonitoring; biomedical informatics; imaging; ICP: brain compliance, biophysics, and biomechanics; stroke, subarachnoid hemorrhage, and intracerebral hematoma; and experimental studies and models. The papers address the increasing use of decompressive craniectomy for the treatment of brain edema as well after brain injury and the rapidly expanding field of advanced neuromonitoring and neuroimaging.
Matrix Metalloproteinase Protocols
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Author : Ian M. Clark
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Matrix Metalloproteinase Protocols written by Ian M. Clark and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
Research in the matrix metalloproteinase field began with the demonstration by Gross and Lapière, in 1962, that resorbing tadpole tail expressed an enzyme that could degrade collagen gels. These humble beginnings have led us to the elucidation of around twenty distinct vertebrate MMPs, along with a variety of homologs from such diverse organisms as sea urchin, plants, nematode worm, and bacteria. This, coupled with four known specific inhibitors of MMPs, the TIMPs, gives a complex picture. Part I of Matrix Metalloproteinase Protocols provides the reader with a selective overview of the MMP arena, and a chance to come to grips with where the field has been, where it is, and where it is going. I hope that this complements all of the methodology that comes later. Part II presents the reader with a diverse set of methods for the expression and purification of MMPs and TIMPs, bringing together the long and often hard-earned experience of a number of researchers. Part III allows the reader to detect MMPs and TIMPs at both the protein and mRNA level, whereas Part IV gives the ability to assay MMP and TIMP activities in a wide variety of circumstances.
Inflammation Protocols
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Author : Paul G. Winyard
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Inflammation Protocols written by Paul G. Winyard and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Medical categories.
Inflammation has been described as the basis of many pathologies of human disease. When one considers the updated signs of inflammation, they would be vasodilation, cell migration, and, in the case of chronic inflam- tion, cell proliferation, often with an underlying autoimmune basis. Gen- ally, inflammation may be divided into acute, chronic, and autoimmune, - though the editors believe that most, if not all, chronic states are often the result of an autoimmune response to an endogenous antigen. Thus, a proper understanding of the inflammatory basis may provide clues to new therap- tic targets not only in classical inflammatory diseases, but atherosclerosis, cancer, and ischemic heart disease as well. The lack of advances in classical inflammatory diseases, such as rh- matoid arthritis, may in part arise from a failure to classify the disease into different forms. That different forms exist is exemplified in patients with d- fering responses to existing antiinflammatory drugs, ranging from nonresponders to very positive responders for a particular nonsteroidal an- inflammatory drug (NSAID). Though researchers have progressively unr- eled the mechanisms, the story is far from complete. It should also be noted that the inflammatory response is part of the innate immune response, or to use John Hunter’s words in 1795, “inflammation is a salutary response.” That may be applied in particular to the defensive response to invading micro- ganisms.
Advances In Enzyme Regulation
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Author : George Weber
language : en
Publisher: Elsevier
Release Date : 2003-07-10
Advances In Enzyme Regulation written by George Weber and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-07-10 with Science categories.
This volume contains papers of 23 outstanding scientists who are working at the leading edge of metabolic regulation studies. Much of the volume focuses on novel aspects of signal transduction with emphasis on the role of phospholipase C in cell control mechanisms. One of the highlights of this volume is always the Special Symposium Lecture. This lecture was given for fifteen years by Nobel laureate, Sir Hans A. Krebs (UK) (now deceased). This year the Lecture was given by Nadir Maraldi, Director of the Laboratory of Cellular Biology and Electron Microscopy, University of Bologna, Italy. Professor Maraldi's presentation is on the important and novel subject of "Nuclear Proteins and Human Disease."
Advances In Clinical Chemistry
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Author :
language : en
Publisher: Elsevier
Release Date : 2004-10-21
Advances In Clinical Chemistry written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-10-21 with Science categories.
Volume thirty-eight in the series of the internationally acclaimed Advances in Clinical Chemistry, contains chapters submitted from leading experts from academia and clinical laboratory science. Authors are from a diverse field of clinical chemistry disciplines and diagnostics ranging from basic biochemical exploration to cutting edge microarray technology. In keeping with the tradition of the series, this volume emphasizes novel laboratory advances with application not only to both clinical laboratory diagnostics, but as well as practical basic science studies.This volume of Advances in Clinical Chemistry is an indispensable resource and practical guide for twenty-first century practitioners of clinical chemistry, molecular diagnostics, pathology, and clinical laboratory sciences in general.Selected Contents on:-Biochemical markers in atherosclerosis and cancer-Molecular diagnostics in metastatic diseases-Single cell mRNA analysis-Protein detecting microarray technology-Evaluation of nerve agents and poisoning
Biochemistry Of Hypertrophy And Heart Failure
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Author : Lorrie A. Kirshenbaum
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Biochemistry Of Hypertrophy And Heart Failure written by Lorrie A. Kirshenbaum and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The focus of this special issue of Molecular and Cellular Biochemistry is underlying mechanisms that regulate cardiac growth. The new information provided in this special issue can be utilized to design new treatment modalities that will reduce the incidence of cardiac failure which will improve quality of life in patients with chronic heart disease.
Handbook Of Detection Of Enzymes On Electrophoretic Gels
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Author : Gennady P. Manchenko
language : en
Publisher: CRC Press
Release Date : 2002-12-26
Handbook Of Detection Of Enzymes On Electrophoretic Gels written by Gennady P. Manchenko and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-12-26 with Medical categories.
Still widely used as gene markers, isozymes detected by zymogram techniques have proven valuable in a range of other biological applications over the last few years. Along with these new applications, many new techniques have also emerged. Yet more than eight years since the Handbook of Detection of Enzymes on Electrophoretic Gels was first publish
Gel Electrophoresis
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Author : Sameh Magdeldin
language : en
Publisher: BoD – Books on Demand
Release Date : 2012-04-04
Gel Electrophoresis written by Sameh Magdeldin and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-04-04 with Science categories.
As a basic concept, gel electrophoresis is a biotechnology technique in which macromolecules such as DNA, RNA or protein are fractionated according to their physical properties such as molecular weight or charge. These molecules are forced through a porous gel matrix under electric field enabling uncounted applications and uses. Delivered between your hands, a second book of this Gel electrophoresis series (Gel Electrophoresis- Advanced Techniques) covers a part, but not all, applications of this versatile technique in both medical and life science fields. We try to keep the contents of the book crisp and comprehensive, and hope that it will receive overwhelming interest and deliver benefits and valuable information to the readers.