A Laboratory Guide To In Vitro Studies Of Protein Dna Interactions
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A Laboratory Guide To In Vitro Studies Of Protein Dna Interactions
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Author : Jost
language : en
Publisher: Birkhäuser
Release Date : 2013-03-13
A Laboratory Guide To In Vitro Studies Of Protein Dna Interactions written by Jost and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-13 with Science categories.
A Safety Considerations Many techniques described here involve a number of hazards, such as high electrical current and voltage, radioactivity and highly toxic chemicals. It is absolutely essential that the instructions of equipment manufacturers be followed, and that particular attention be paid to the local and federal safety regulations. B Introduction The expression of prokaryotic and eukaryotic genes has been shown most often to be regulated at the level of mRNA synthesis. Thanks to the rapid development of methods for dissecting DNA sequences, cis-acting regulatory elements such as promoters and enhancers have been recognised. More recently, the widely expressed intuition that discrete sequences within these elements constitute binding sites for sequence-specific binding proteins has been confirmed, especially through the use of "footprinting" assays (for examples, Galas and Schmitz, 1978). This and similar assays have already resulted in the recognition, isolation and analysis of DNA-bind ing proteins for several genes. Excellent reviews exist of the structural studies on these transcription regulatory proteins and related DNA elements (for example, Glover, 1989 and Johnson and McKnight, 1989), to which the reader is referred for detailed information. To set the scene for applications of the techniques described in this volume, only the barest outline of previous studies is presented here. Protein-DNA interactions are dependent on very specific tertiary configurations of the binding protein which allow the closest contact with the DNA helix.
A Laboratory Guide To In Vitro Studies Of Protein Dna Interactions
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Author : Jost
language : en
Publisher: Birkhäuser
Release Date : 1991-09-01
A Laboratory Guide To In Vitro Studies Of Protein Dna Interactions written by Jost and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 1991-09-01 with Science categories.
A Safety Considerations Many techniques described here involve a number of hazards, such as high electrical current and voltage, radioactivity and highly toxic chemicals. It is absolutely essential that the instructions of equipment manufacturers be followed, and that particular attention be paid to the local and federal safety regulations. B Introduction The expression of prokaryotic and eukaryotic genes has been shown most often to be regulated at the level of mRNA synthesis. Thanks to the rapid development of methods for dissecting DNA sequences, cis-acting regulatory elements such as promoters and enhancers have been recognised. More recently, the widely expressed intuition that discrete sequences within these elements constitute binding sites for sequence-specific binding proteins has been confirmed, especially through the use of "footprinting" assays (for examples, Galas and Schmitz, 1978). This and similar assays have already resulted in the recognition, isolation and analysis of DNA-bind ing proteins for several genes. Excellent reviews exist of the structural studies on these transcription regulatory proteins and related DNA elements (for example, Glover, 1989 and Johnson and McKnight, 1989), to which the reader is referred for detailed information. To set the scene for applications of the techniques described in this volume, only the barest outline of previous studies is presented here. Protein-DNA interactions are dependent on very specific tertiary configurations of the binding protein which allow the closest contact with the DNA helix.
A Laboratory Guide For In Vivo Studies Of Dna Methylation And Protein Dna Interactions
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Author : Saluz
language : en
Publisher:
Release Date : 2014-01-15
A Laboratory Guide For In Vivo Studies Of Dna Methylation And Protein Dna Interactions written by Saluz and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-15 with categories.
A Laboratory Guide For In Vivo Studies Of Dna Methylation And Protein Dna Interactions
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Author : Saluz
language : en
Publisher: Birkhäuser
Release Date : 2013-03-09
A Laboratory Guide For In Vivo Studies Of Dna Methylation And Protein Dna Interactions written by Saluz and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Science categories.
A Laboratory Guide To In Vitro Transcription
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Author : Felipe Sierra
language : en
Publisher: Birkhauser
Release Date : 1990
A Laboratory Guide To In Vitro Transcription written by Felipe Sierra and has been published by Birkhauser this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990 with Science categories.
Dna Protein Interactions
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Author : Tom Moss
language : en
Publisher: Humana
Release Date : 2010-11-19
Dna Protein Interactions written by Tom Moss and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-19 with Science categories.
Gene expression can mean the difference between a functional and non-functional genome, between health and disease, and with the development of transgenic crops, the difference between survival and starvation. In DNA-Protein Interactions: Principles and Protocols, Third Edition, this vital subject is brought up to date with protocols exploring the most cutting-edge developments in the field, including in vivo and genome-wide interaction techniques. Addressing topics such as chromatin immunoprecipitation, topological studies, photocrosslinking, FRET and imaging techniques, the volume fully updates and expands upon the successful previous editions. Written in the convenient and informative Methods in Molecular BiologyTM series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, DNA-Protein Interactions: Principles and Protocols, Third Edition serves as an ideal guide for all those exploring this dynamic, essential, and increasingly affordable area of research.
A Laboratory Guide For Cellular And Molecular Plant Biology
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Author : I. Negrutiu
language : en
Publisher: Birkhäuser
Release Date : 2013-03-09
A Laboratory Guide For Cellular And Molecular Plant Biology written by I. Negrutiu and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Science categories.
This laboratory guide comes at a time when several other method books have already been published in this field. Is this one different from the others? Yes and no. There was no attempt made to be comprehensive. Rather, data were brought to bear on areas where enough competence has been gathered in our laboratories and to complement recent method books (many of which cover extensively various aspects of molecular biology) in those matters which appeared to us somewhat neglected. There was a constant preoccupation and effort to provide miniaturized proce dures that are both simple and time-saving. Interest was devoted to standardized procedures and culture conditions, avoiding dogmas such as those giving excessive importance to sophisticated culture media with endless adjustments for local or personal considerations. The key to success is the quality of the plant material serving as a source of cells. Consequently, isolation. extraction or culture techniques can be simplified and standardized. This is symptomatic for our times as it marks the end of a period when methodological matters were frequently above the biological problems. The times of "methods above all" is basically over, despite the fact that many of us still believe that, say, tissue culture is a "science" per se. By presenting a few original techniques we believe that one seriously reduces the empiricism still prevailing in this area of research.
Protein Protein Interactions
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Author : Shahid Mukhtar
language : en
Publisher: Springer Nature
Release Date : 2023-07-14
Protein Protein Interactions written by Shahid Mukhtar and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-07-14 with Science categories.
This detailed volume provides a comprehensive collection of classic and cutting-edge methods and techniques in mapping protein-protein interactions. The chapters include a variety of in vitro and in vivo experimental methods covering cell biology, biochemistry, and biophysics. In addition, the book also explores in silico methods including sequence-, structure-, and phylogenetic profile-based approaches as well as gene expression and machine learning methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protein-Protein Interactions: Methods and Protocols serves as an ideal guide for researchers working in protein science and beyond.
Rna Protein Interactions A Practical Approach
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Author : Christopher W.J. Smith
language : en
Publisher: Oxford University Press, UK
Release Date : 1998-07-09
Rna Protein Interactions A Practical Approach written by Christopher W.J. Smith and has been published by Oxford University Press, UK this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-07-09 with categories.
RNA-protein interactions play a fundamental role in gene expression and protein synthesis. Recent research into the role of RNA in cells has elucidated many more vital interactions with proteins. This book provides an up-to-date and comprehensive guide to a wide range of laboratory procedures to investigate the interactions between RNA and proteins. - ;RNA-protein interactions play a vital role in gene transcription and protein expression. Interactions such as the synthesis of mRNA by RNA polymerases, to the essential modification of RNA by the proteins of the spliceosome complex, and the highly catalytic action of the ribosome in protein synthesis, are established as being fundamental to the function of RNA. Recent research into, for example, the role of RNA as a catalyst, has elucidated many more interactions with proteins that are vital to cell function. RNA - Protein Interactions: A Practical Approach provides a clear and comprehensive guide to the experimental procedures used in studying RNA - protein interactions. The approaches covered range from those initially used to detect a novel RNA-protein interaction, various biochemical and genetic approaches to purifying and cloning RNA binding proteins, through to methods for an in depth analysis of the structural basis of the interaction. The volume includes a number of procedures that have not previously been covered in this type of manual. These include the production of site-specifically modified RNAs by enzymatic and chemical methods and in vivo screening for novel RNA - protein interactions in yeast and E. coli . This is the first volume to gather in one place this wide array of approaches for studying RNA - protein interactions. As is customary for the Practical Approach series, the writing is characterized by a clear explanatory style with many detailed protocols. This informative book will be a valuable aid to laboratory workers in biochemistry and molecular biology - graduate students, postdoctoral and senior scientists - whose research encompasses this field. -
A Laboratory Guide To In Vitro Transcription
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Author : F. Sierra
language : en
Publisher: Birkhäuser
Release Date : 2013-11-21
A Laboratory Guide To In Vitro Transcription written by F. Sierra and has been published by Birkhäuser this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-21 with Science categories.