A Novel Wide Field Time Correlated Single Photon Counting Technique For Luminescence Lifetime Imaging
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A Novel Wide Field Time Correlated Single Photon Counting Technique For Luminescence Lifetime Imaging
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Author : Nicolas Sergent
language : en
Publisher:
Release Date : 2009
A Novel Wide Field Time Correlated Single Photon Counting Technique For Luminescence Lifetime Imaging written by Nicolas Sergent and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with Fluorescence categories.
This Thesis presents the development and characterisation of a novel wide field, time-correlated single photon technique for luminescence lifetime imaging, and its possible use for life sciences imaging. -- The primary device which was developed is based on a 3-stage image intensifier coupled with a high speed CMOS camera, mounted on a conventional microscope. The post-acquisition image processing software developed for this purpose, in¬volving photon detection and centroiding algorithms, is described and discussed. Lifetime images of artificial ruby and europium compounds are reported, as well as other applications of single photon counting imaging for fluorescence microscopy. Another wide-field TCSPC technique, based on an Electron-Bombarded CCD de¬tector, is presented, along with the characterisation of the device, and its uses as a single photon counting detector for wide-field microscopy. -- This work also presents results of Fluorescence Lifetime Imaging Microscopy and Time-Resolved Anisotropy measurements of biological samples on a commercial laser scanning microscope system. -- Finally, this work presents suggestions of improvement and further developments on these wide-field TCSPC detectors.
Devleopment And Application Of Photon Counting Techniques For Fluorescence Microscopy
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Author :
language : en
Publisher:
Release Date : 2015
Devleopment And Application Of Photon Counting Techniques For Fluorescence Microscopy written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
Fluorescence lifetime imaging microscopy (FLIM) is a key technique to image cells as, in addition to the advantages of standard fluorescence microscopy, it allows to study the environment and probe interaction in living specimens. Implementing FLIM via Single Photon Counting (SPC) proved to be the most effective technique considering the fiuorophores limited photon budget before being irreversibly bleached. This thesis focuses on the development and application of Single Photon Counting techniques to imaging systems and to spectroscopy. , Firstly, the BODIPY-C12 molecular rotor was used to determine dyeconcentrations between 3 and 16 ~tM for living cells and lipid droplets via FLIM and intensity measurements A novel compound, named ET, was tested for the first time on living cells and its possible applications as a molecular rotor discussed. The use of an Electron Bombarded Charge-Coupled Device (EBCCD) camera as a parallel-processing Time to Amplitude Converter device for SPC Imaging with sub-frame exposure time resolution was investigated and, although not implemented, the results supports the proposed method. In order to design a wide field time-correlated single photon counting system, a Complementary Metal-Oxide Semiconductor (CMOS) FastCamera was coupled with an 3-stage image intensifier. This proved to be suitable for luminescence lifetime measurements of a Ruthenium complex, with results of 1. 7~ts comparable with confocal scanning Time-Correlated SPC (TCSPC). Finally, conclusions and future work are discussed.
Time Correlated Single Photon Counting
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Author : Desmond O'Connor
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Time Correlated Single Photon Counting written by Desmond O'Connor and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time dependence and applications of fluorescence. Succeeding chapters go on to discuss basic principles of the single photon counting lifetime measurement; light sources; photomultipliers; electronics; data analysis; nanosecond time-resolved emission spectroscopy; time dependence of fluorescence anisotropy. This book will be of interest to practitioners in the field of chemistry.
Handbook Of Photonics For Biomedical Engineering
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Author : Aaron H.-P. Ho
language : en
Publisher: Springer
Release Date : 2016-09-29
Handbook Of Photonics For Biomedical Engineering written by Aaron H.-P. Ho and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-09-29 with Technology & Engineering categories.
Nanophotonics has emerged rapidly into technological mainstream with the advent and maturity of nanotechnology available in photonics and enabled many new exciting applications in the area of biomedical science and engineering that were unimagined even a few years ago with conventional photonic engineering techniques. Handbook of Nanophotonics in Biomedical Engineering is intended to be a reliable resource to a wealth of information on nanophotonics that can inspire readers by detailing emerging and established possibilities of nanophotonics in biomedical science and engineering applications. This comprehensive reference presents not only the basics of nanophotonics but also explores recent experimental and clinical methods used in biomedical and bioengineering research. Each peer-reviewed chapter of this book discusses fundamental aspects and materials/fabrication issues of nanophotonics, as well as applications in interfaces, cell, tissue, animal studies, and clinical engineering. The organization provides quick access to current issues and trends of nanophotonic applications in biomedical engineering. All students and professionals in applied sciences, materials, biomedical engineering, and medical and healthcare industry will find this essential reference book highly useful.
High Performance Silicon Imaging
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Author : Daniel Durini
language : en
Publisher: Woodhead Publishing
Release Date : 2019-10-19
High Performance Silicon Imaging written by Daniel Durini and has been published by Woodhead Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-10-19 with Technology & Engineering categories.
High Performance Silicon Imaging: Fundamentals and Applications of CMOS and CCD Sensors, Second Edition, covers the fundamentals of silicon image sensors, addressing existing performance issues and current and emerging solutions. Silicon imaging is a fast growing area of the semiconductor industry. Its use in cell phone cameras is already well established, with emerging applications including web, security, automotive and digital cinema cameras. The book has been revised to reflect the latest state-of-the art developments in the field, including 3D imaging, advances in achieving lower signal noise, and new applications for consumer markets. The fundamentals section has also been expanded to include a chapter on the characterization and testing of CMOS and CCD sensors that is crucial to the success of new applications. This book is an excellent resource for both academics and engineers working in the optics, photonics, semiconductor and electronics industries. Covers the fundamentals of silicon-based image sensors and technical advances, focusing on performance issues Looks at image sensors in applications, such as mobile phones, scientific imaging, and TV broadcasting, and in automotive, consumer and biomedical applications Addresses the theory behind 3D imaging and 3D sensor development, including challenges and opportunities
Modern Tools For Time Resolved Luminescence Biosensing And Imaging
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Author :
language : en
Publisher: Frontiers Media SA
Release Date : 2021-09-01
Modern Tools For Time Resolved Luminescence Biosensing And Imaging written by and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-09-01 with Science categories.
Imaging From Cells To Animals In Vivo
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Author : Margarida Barroso
language : en
Publisher: CRC Press
Release Date : 2020-12-03
Imaging From Cells To Animals In Vivo written by Margarida Barroso and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-12-03 with Science categories.
Imaging from Cells to Animals In Vivo offers an overview of optical imaging techniques developed over the past two decades to investigate biological processes in live cells and tissues. It comprehensively covers the main imaging approaches used as well as the application of those techniques to biological investigations in preclinical models. Among the areas covered are cell metabolism, receptor-ligand interactions, membrane trafficking, cell signaling, cell migration, cell adhesion, cytoskeleton and other processes using various molecular optical imaging techniques in living organisms, such as mice and zebrafish. Features Brings together biology and advanced optical imaging techniques to provide an overview of progress and modern methods from microscopy to whole body imaging. Fills the need for a comprehensive view of application-driven development and use of new tools to ask new biological questions in the context of a living system. Includes basic chapters on key methods and instrumentation, from fluorescence microscopy and imaging to endoscopy, optical coherence tomography and super-resolution imaging. Discusses approaches at different length scales and biomedical applications to the study of single cell, whole organ, and whole organism behavior. Addresses the impact on discovery, such as cellular function as implicated in human disease and translational medicine, for example in cancer diagnosis. Margarida M. Barroso is a Professor in the Department of Molecular and Cellular Physiology, Albany Medical College (Albany, New York). Xavier Intes is a Professor in the Biomedical Engineering Department and Co-Director of the Center for Modeling, Simulation and Imaging for Medicine (CeMSIM) at Rensselaer Polytechnic Institute (Troy, New York).
Advanced Time Correlated Single Photon Counting Techniques
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Author : Wolfgang Becker
language : en
Publisher: Springer Science & Business Media
Release Date : 2005-12-19
Advanced Time Correlated Single Photon Counting Techniques written by Wolfgang Becker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-12-19 with Science categories.
In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor.
Progress In Photon Science
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Author : Kaoru Yamanouchi
language : en
Publisher: Springer
Release Date : 2019-01-23
Progress In Photon Science written by Kaoru Yamanouchi and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-01-23 with Science categories.
This second volume of “Progress in Photon Science – Recent Advances” presents the latest achievements made by world-leading researchers in Russia and Japan. Thanks to recent advances in light source technologies; detection techniques for photons, electrons, and charged particles; and imaging technologies, the frontiers of photon science are now being expanding rapidly. Readers will be introduced to the latest research efforts in this rapidly growing research field through topics covering bioimaging and biological photochemistry, atomic and molecular phenomena in laser fields, laser–plasma interaction, advanced spectroscopy, electron scattering in laser fields, photochemistry on novel materials, solid-state spectroscopy, photoexcitation dynamics of nanostructures and clusters, and light propagation.
Principles Of Fluorescence Spectroscopy
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Author : Joseph R. Lakowicz
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Principles Of Fluorescence Spectroscopy written by Joseph R. Lakowicz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.